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Composition and use thereof, umbilical cord preservation preparation and preparation method thereof

A preservation method and composition technology, applied in the medical field, can solve the problems of umbilical cord preservation quality fluctuations, no stable buffer solution system, and single nutritional components, so as to maintain the original cell morphology and biological characteristics, and maintain the vitality of seed cells , The effect of simple preparation steps

Active Publication Date: 2018-02-09
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. The preservation solution is composed of a basic medium with a single nutritional component, and this component may not be suitable for the needs of the umbilical cord in vitro
[0006] 2. There is no stable buffer solution system, the storage process is greatly affected by external factors, and the quality of umbilical cord storage fluctuates greatly
[0007] 3. The configuration process is cumbersome

Method used

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  • Composition and use thereof, umbilical cord preservation preparation and preparation method thereof
  • Composition and use thereof, umbilical cord preservation preparation and preparation method thereof
  • Composition and use thereof, umbilical cord preservation preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Preparation of preservation solution:

[0046] In normal saline, add double-antibody, lacturonic acid, hydroxyethyl starch, and umbilical cord plasma, so that normal saline contains 1x penicillin-streptomycin (double-antibody), the final concentration is 100 mg / mL lacturonic acid, and the final concentration is 1% (v / v) hydroxyethyl starch and umbilical cord plasma with a final concentration of 5% (v / v) were mixed and stored at 4°C for later use.

Embodiment 2

[0047] Embodiment 2 Preparation of preservation solution:

[0048]In normal saline, add double antibody, lacturonic acid, hydroxyethyl starch, umbilical cord plasma, make the normal saline contain 2x penicillin-streptomycin (double antibody), final concentration is 50mg / mL lacturonic acid, final concentration is 5% (v / v) hydroxyethyl starch and umbilical cord plasma with a final concentration of 5% (v / v) were mixed and stored at 4°C for later use.

Embodiment 3

[0049] Embodiment 3 The preparation of preservation solution:

[0050] In normal saline, add double antibody, lacturonic acid, hydroxyethyl starch, umbilical cord plasma, make the normal saline contain 1x penicillin-streptomycin (double antibody), final concentration is 80mg / mL lacturonic acid, final concentration is 3% (v / v) hydroxyethyl starch and umbilical cord plasma with a final concentration of 5% (v / v) were mixed and stored at 4°C for later use.

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Abstract

The invention relates to the medical field, particularly to a composition and its use, an umbilical cord preservation preparation and a preparation method thereof. According to the invention, physiological saline is taken as the basic ingredient of umbilical cord preservation liquid, umbilical cord blood plasma is used as the nutritional ingredient source, as a natural substance, umbilical cord blood plasma not only provides multiple nutritional ingredients, but also can maximumly maintain the umbilical cord vitality in order to maintain a microenvironment similar to that in vivo. Lactobionic acid, hydroxyethyl starch and other macromolecular substances are added to avoid swelling death of the umbilical cord in the preservation process. The preservation liquid can be used for transportation and preservation of the preparation at low temperature. Thus, adequate nutrients are supplied to in vitro umbilical cord, and also the seed cell vitality, the original cell morphology and biological characteristics are well maintained.

Description

technical field [0001] The invention relates to the medical field, in particular to a composition and its application, an umbilical cord preservation preparation and a preparation method thereof. Background technique [0002] Umbilical cord mesenchymal stem cells (MSCs) have high differentiation potential and can differentiate in multiple directions. It has broad clinical application prospects in tissue engineering of bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle. It has been reported that MSCs isolated from human umbilical cord have better cell content and proliferation ability than bone marrow MSCs, lower immunogenicity than bone marrow MSCs, and have the advantages of convenient material extraction and no ethical controversy. s concern. [0003] However, the viability of the umbilical cord will gradually decrease in vitro, which directly affects the quantity and quality of the isolated stem cells. How to maintain the activity o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 陈海佳王一飞葛啸虎卢瑞珊王小燕李平
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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