Swine pasteurellosis vaccine enrichment medium

An enrichment culture medium, a technology for swine pneumonia, which is applied in the field of biology, can solve the problems of low survival rate of bacterin freeze-drying, low number of cultured bacteria, precipitation in the culture medium, etc. The effect of high bacterial count and easy operation

Pending Publication Date: 2020-04-21
青岛高科技工业园海博生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a porcine pneumonia vaccine enrichment medium, which solves the technical problems in the prior art that there is precipitation after the medium is sterilized, the number of cultured bacteria is low, and the survival rate of the freeze-dried bacteria is low.

Method used

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  • Swine pasteurellosis vaccine enrichment medium
  • Swine pasteurellosis vaccine enrichment medium
  • Swine pasteurellosis vaccine enrichment medium

Examples

Experimental program
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Effect test

Embodiment 1

[0019] A porcine pneumonia vaccine enrichment medium, comprising the following raw materials in parts by weight, 10.0 g of meat liver and stomach membrane digestion liquid powder, 5.0 g of peptone, 3.0 g of NaCl, 7.0 g of bovine pancreas digest and 0.5 g of growth-promoting factor, purified water Add to 1000ml.

[0020] Wherein the growth-promoting factor comprises amino acid, trace salt ion and trace element, and described amino acid comprises L-glutamic acid 0.001g and p-aminobenzoic acid 0.001g, and described trace salt ion comprises dipotassium hydrogen phosphate 0.2674g, potassium dihydrogen phosphate 0.2000g, NH 4 Cl 0.01g, magnesium sulfate 0.01g and zinc sulfate 0.01g, said trace elements include niacin 0.0002g, calcium pantothenate 0.0002g, biotin 0.0002g.

[0021] Base preparation method: add all the raw materials used to prepare porcine pneumonia vaccine medium into purified water, fully dissolve, sterilize at 121°C for 15 minutes, and set aside.

Embodiment 2

[0023] A porcine pneumonia vaccine enrichment medium, comprising the following raw materials in parts by weight, 15.0 g of meat liver and gastric membrane digestion liquid powder, 8.0 g of peptone, 4.0 g of NaCl, 9.0 g of bovine pancreas digest and 0.8 g of growth-promoting factor, purified water Add to 1000ml.

[0024] Wherein the growth-promoting factor comprises amino acid, trace salt ion and trace element, and described amino acid comprises L-glutamic acid 0.003g and p-aminobenzoic acid 0.003g, and described trace salt ion comprises dipotassium hydrogen phosphate 0.3437g, potassium dihydrogen phosphate 0.3000g, NH 4 Cl 0.05g, magnesium sulfate 0.05g and zinc sulfate 0.05g, said trace elements include niacin 0.0001g, calcium pantothenate 0.0001g, biotin 0.0001g.

[0025] Base preparation method: add all the raw materials used to prepare porcine pneumonia vaccine medium into purified water, fully dissolve, sterilize at 121°C for 15 minutes, and set aside.

Embodiment 3

[0027] A porcine pneumonia vaccine enrichment medium, comprising the following raw materials in parts by weight, 20.0 g of meat liver and gastric membrane digestion liquid powder, 10.0 g of peptone, 5.0 g of NaCl, 8.0 g of bovine pancreas digest and 1.0 g of growth-promoting factor, purified water Add to 1000ml.

[0028] Wherein the growth-promoting factor comprises amino acid, trace salt ion and trace element, and described amino acid comprises L-glutamic acid 0.002g and p-aminobenzoic acid 0.002g, and described trace salt ion comprises dipotassium hydrogen phosphate 0.5g, potassium dihydrogen phosphate 0.4g, NH4Cl 0.03g, magnesium sulfate 0.033g and zinc sulfate 0.03g, the trace elements include niacin 0.001g, calcium pantothenate 0.001g, biotin 0.001g.

[0029] Base preparation method: add all the raw materials used to prepare porcine pneumonia vaccine medium into purified water, fully dissolve, sterilize at 121°C for 15 minutes, and set aside.

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Abstract

The invention provides a swine pasteurellosis vaccine enrichment medium, and relates to the field of biology. The swine pasteurellosis vaccine enrichment medium is prepared from the following raw materials by weight: 20.0g of meat liver and stomach membrane digestive juice powder, 8.0g of bovine pancreas digestive juice, 10.0g of peptone, 5.0g of NaCl, 1.0g of a growth promoting factor, and purified water added to 1000ml. A preparation method of the swine pasteurellosis vaccine enrichment medium comprises the following steps: adding all the raw materials for preparing the swine pasteurellosisvaccine enrichment medium into purified water, fully dissolving the raw materials, and carrying out sterilization at 121 DEG C for 15 minutes for later use. The invention relates to an application ofa synthetic dry powder culture medium in swine pasteurellosis vaccine production. The synthetic dry powder culture medium has the beneficial effects that commercial raw materials can be selected, theculture medium is clear, the number of cultured bacteria is high, and the freeze-drying survival rate of the bacteria is increased.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to a culture medium for porcine pneumonia vaccine enrichment. Background technique [0002] Swine pneumonia, also known as swine pasteurellosis (Swinepasteurellosis), is one of the three major infectious diseases of pigs. It is distributed worldwide and is also a zoonosis. Its source of disease is Pasteurella multocida. At present, the existing porcine lung disease vaccines in my country include single vaccine, double vaccine and triple vaccine, which play an important role in preventing porcine lung disease. However, due to the complex serotypes of pathogenic bacteria, there is a lack of ideal vaccines at home and abroad, especially in the aspect of enrichment and cultivation of vaccine bacteria liquid. [0003] In recent years, judging from the production and research of traditional routine vaccines, various manufacturers have made more efforts in terms of strains and production ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 蔡向荣许英俊
Owner 青岛高科技工业园海博生物技术有限公司
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