Immune cell cryopreservation liquid and immune cell cryopreservation method
A technology of immune cells and cryopreservation solution, which is applied in the preservation, application, and animal husbandry of human or animal bodies. It can solve problems such as allergic reactions, immune rejection, and infectious diseases, and achieve good cell vitality and change permeability. sex, the effect of meeting the needs of immune cells
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Embodiment 1
[0031] An immune cell cryopreservation solution, the components of which include:
[0032] Dimethyl sulfoxide 0.02mg / mL,
[0033] Cucurbitacin 1mg / mL,
[0034] Acacia polysaccharide 0.01mL / mL,
[0035] Lentinan 1mg / mL,
[0036] Polyglycerol fatty acid ester 1 μg / mL,
[0037] Polyethylene glycol 0.01mg / mL,
[0038] Non-essential amino acids 10mg / mL,
[0039] Human serum albumin 12mg / mL.
[0040] A method for cryopreserving immune cells, comprising the steps of:
[0041] S1. Take peripheral blood in a centrifuge tube, centrifuge for 10 minutes, remove the upper layer of plasma, add an equal volume of PBS buffer to the lower layer of cells and mix evenly, then add Ficoll separation solution to make the layers clear, absorb the peripheral blood mononuclear cell layer after centrifugation, add PBS buffer was mixed and centrifuged to obtain peripheral blood mononuclear cells;
[0042] S2. Mix the peripheral blood mononuclear cells with the above-mentioned immune cell cryopre...
Embodiment 2
[0045] An immune cell cryopreservation solution, the components of which include:
[0046] Dimethyl sulfoxide 0.06mg / mL,
[0047] Cucurbitacin 6mg / mL,
[0048] Acacia polysaccharide 0.03mL / mL,
[0049] Lentinan 2mg / mL,
[0050] Polyglycerol fatty acid ester 5μg / mL,
[0051] Polyethylene glycol 0.03mg / mL,
[0052] Non-essential amino acids 16mg / mL,
[0053] Human serum albumin 20mg / mL.
[0054] A method for cryopreserving immune cells, comprising the steps of:
[0055] S1. Take peripheral blood in a centrifuge tube, centrifuge for 20 minutes, remove the upper layer of plasma, add an equal volume of PBS buffer to the lower layer of cells and mix evenly, then add Ficoll separation solution to make the layers clear, absorb the peripheral blood mononuclear cell layer after centrifugation, add PBS buffer was mixed and centrifuged to obtain peripheral blood mononuclear cells;
[0056] S2. Mix the peripheral blood mononuclear cells with the above-mentioned immune cell cryopres...
Embodiment 3
[0059] An immune cell cryopreservation solution, the components of which include:
[0060] Dimethyl sulfoxide 0.03mg / mL,
[0061] Cucurbitacin 5mg / mL,
[0062]Acacia polysaccharide 0.015mL / mL,
[0063] Lentinan 1.7mg / mL,
[0064] Polyglycerol fatty acid ester 2μg / mL,
[0065] Polyethylene glycol 0.025mg / mL,
[0066] Non-essential amino acids 12mg / mL,
[0067] Human serum albumin 18mg / mL.
[0068] A method for cryopreserving immune cells, comprising the steps of:
[0069] S1. Take peripheral blood in a centrifuge tube, centrifuge for 13 minutes, remove the upper layer of plasma, add an equal volume of PBS buffer to the lower layer of cells and mix evenly, then add Ficoll separation solution to make the layers clear, absorb the peripheral blood mononuclear cell layer after centrifugation, add PBS buffer was mixed and centrifuged to obtain peripheral blood mononuclear cells;
[0070] S2. Mix the peripheral blood mononuclear cells with the above-mentioned immune cell cryop...
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