Bletilla striata rapid propagation and sunshine seedling strengthening method

A sunny and fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low reproductive efficiency, small seeds, large seed consumption, etc., and achieve good development, uniform size, and moderate height of seedlings Effect

Inactive Publication Date: 2015-09-16
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional cultivation of Baiji mainly relies on ramet propagation, which has a long reproductive cycle, low reproductive efficiency, and large seed consumption, which is difficult to meet the needs of a large number of planting. The seeds of Baiji are small, underdeveloped, and have no endosperm, so it is difficult to germinate under natural conditions. , but the germination and growth of Bletilla seeds are faster on the artificial medium, so the use of plant tissue culture technology can quickly breed a large number of Bletilla seedlings, which is of great significance to the utilization of Bletilla resources, protection and artificial large-scale and intensive planting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] a. Seed induction germination culture: Harvest uncracked capsules from July to September every year, clean the surface of the capsules with soapy water, soak them in 75% alcohol for 30 seconds on a sterile workbench, and then use Soak in 0.1% mercuric chloride solution for 18 minutes, then rinse with sterile water for 5 times, and finally use sterile filter paper to absorb the water on the surface of the capsule, cut off the two ends of the capsule with a sterile scalpel, and cut the capsule from the middle. Split into two halves, sow the seeds taken from the Baiji capsules on the seed induction germination medium, and cultivate them for 55 days at a temperature of 25°C and a light intensity of 2400Lux, wherein the light time for the first 25 days is 6 hours / day, and then The light time for 30 days was 10 hours / day to obtain seedlings; the density of seed sowing was 400-500 seeds per culture bottle.

[0023] b. Rooting culture: put plant height > 2cm seedlings on the ro...

Embodiment 2

[0027] a. Seed induction germination culture: Harvest uncracked capsules from July to September every year, clean the surface of the capsules with soapy water, soak them in 75% alcohol for 30 seconds on a sterile workbench, and then use Soak in 0.1% mercuric chloride solution for 20 minutes, then rinse with sterile water for 6 times, and finally use sterile filter paper to absorb the water on the surface of the capsule, cut off the two ends of the capsule with a sterile scalpel, and cut the capsule from the middle. Split into two halves, sow the seeds taken from the Baiji capsules on the seed induction germination medium, and cultivate them for 60 days at a temperature of 27°C and a light intensity of 2600Lux. The light time for the first 30 days is 6 hours / day, and then The light time for 30 days was 10 hours / day to obtain seedlings; the density of seed sowing was 400-500 seeds per culture bottle.

[0028] b. Rooting culture: put the plant height > 2cm seedlings on the rootin...

Embodiment 3

[0032] a. Seed induction germination culture: Harvest uncracked capsules from July to September every year, clean the surface of the capsules with soapy water, soak them in 70% alcohol for 30 seconds on a sterile workbench, and then use Soak in 0.1% mercuric chloride solution for 15 minutes, then rinse with sterile water for 4 times, and finally use sterile filter paper to absorb the water on the surface of the capsule, cut off both ends of the capsule with a sterile scalpel, and cut the capsule from the middle. Split into two halves, sow the seeds taken from the Baiji capsules on the seed induction germination medium, and cultivate them for 50 days at a temperature of 23°C and a light intensity of 2000Lux, wherein the light time for the first 25 days is 6 hours / day, and then The light time for 25 days was 10 hours / day to obtain seedlings; the seed sowing density was 400-500 seeds per culture bottle.

[0033] b. Rooting culture: place the seedlings with a plant height > 2cm on...

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PUM

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Abstract

The present invention relates to a bletilla striata rapid propagation and sunshine seedling strengthening method belonging to the technical field of bletilla striata artificial cultivation. The method comprises the following steps: a, seed induced germination culture, to be more specific, seeds taken from bletilla striata capsules are sowed onto a seed induced germination medium for culturing for 50 days to obtain seedlings; b, rooting culture, to be more specific, the seedlings are put on a rooting medium for culturing for 40 days at the temperature of 23-27 DEG C under the light intensity of 2000-2600Lux to obtain rooting seedlings; and c, sunshine seedling strengthening, to be more specific, the rooting seedlings are transferred to a sunshine greenhouses for culturing for 10 days at the temperature of 20-28 DEG C under the light intensity of 3000-4000Lux, and then the light intensity is adjusted to 5000-6000Lux for culturing for 20 to 25 days. The beneficial effects of the bletilla striata rapid propagation and sunshine seedling strengthening method is that: the bletilla striata rapid propagation and sunshine seedling strengthening method can greatly improve seedling and seed quality, the seedling and seed are thick and solid, and even, the planting survival rate is high, and the survival rate reaches over 95%.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of Radix Radix, and in particular relates to a method for rapid propagation and sunshine seedling growth of Radix Radix. Background technique [0002] White and (Bletilla striata (Thunb) Reichb.f) is a perennial herb belonging to Orchidaceae, widely distributed. The tuber of Baiji is a traditional Chinese medicine in my country, which is recorded in "Shen Nong's Materia Medica". In addition, Baiji has bright colors and is also an ornamental plant. The market demand for Baiji is expanding year by year. Natural resources are drastically reduced and are on the verge of extinction. It is listed as one of the wild medicinal plants under key protection by the country. It is imperative to protect the wild natural resources of Baiji and artificially propagate and plant Baiji. The traditional cultivation of Baiji mainly relies on ramet propagation, which has a long reproductive cycle, low r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨宝明李永平黄玉玲
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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