Tissue culture quick propagation method of polygonatum kingianum

Abstract

The invention discloses a tissue culture quick propagation method of polygonatum kingianum, which includes the steps of: 1) selecting and sterilizing explants; 2) inoculating the explants onto an induced differentiation culture medium, and culturing the explants for 40-50 days with the light intensity being 1800-2000 Lx, irradiating period being 8-10 h per day and culture temperature being 25-27 DEG C; 3) cutting the tissue, which has adventitious buds and projections, generated in the induced differentiation culture medium into dices, and inoculating the dices onto a proliferation culture medium to perform proliferation cultivation to achieve proliferation of adventitious buds; 4) cutting the buds, of which the cluster buds are not less than 0.5 cm in diameter and not less than 3 cm in height, obtained in the proliferation cultivation into single bodies with leaves cut away, inoculating the single bodies on a rooting culture medium, and culturing the single bodies for 30-35 days with the light intensity being 1800-2000 Lx, irradiating period being 10 h per day at the first 20 days and 12 h per day later, and culture temperature being 25-27 DEG C to obtain rooting seedlings; and 5) performing domestication and seedling hardening. The method is more than 95% in survival rate.

Description

technical field [0001] The invention belongs to the technical field of medicinal material planting, and relates to a method for tissue culture and rapid propagation of Polygonatum yunnanensis. Background technique [0002] Polygonatum kingianum (polygonatum kingianum.coll.ethms) is a herbaceous plant of the genus Polygonatum in the family Liliaceae. It is mainly produced in Yunnan, Sichuan, Guizhou and other places in China, and grows in forests, bushes or shady slope wetlands. Its rhizomes are used as medicine to invigorate the middle and nourish Qi, nourish the lungs and nourish yin, nourish the kidney and essence, eliminate rheumatism, calm the five internal organs, and inhibit tumor cells. With the continuous deepening of research, people also affirmed and recognized the medicinal value and health care efficacy of sealwort, and it has broad prospects in the research of new drugs and the development of health care products. The market demand for raw materials is increasi...

AI Technical Summary

Problems solved by technology

The other is asexual reproduction through rhizomes. Because Polygonatum yunnanensis seeds are difficult to collect, the germination rate is low, and the growth cycle of seedlings is long, which increases the cost of production and planting, which is not conducive to large-scale promotion of planting

The method of rhizome propagation has a low reproduction coefficient, and the large amount of rhizomes used is not economical, but also limits the scale of production and planting. The problem of seedlings has become a bottleneck restricting the large-scale artificial planting of Rhizoma Polygonatum.