23 results about "Cucurbita pepo" patented technology

The invention provides an SNP (Single Nucleotide Polymorphism) site combination, a molecular probe combination, a zucchini whole genome gene chip and application thereof, the SNP site combination comprises at least one SNP site in a table 1, and physical position information of the SNP site in the table 1 is determined based on a zucchini reference genome 22S20 or genome sequence comparison of an updated version of the zucchini reference genome 22S20. Specifically, the invention provides a zucchini whole genome 10K liquid-phase gene chip and application thereof. By adopting the zucchini whole genome 10K liquid-phase gene chip disclosed by the invention, high-depth re-sequencing can be carried out on target sites of zucchini, and accurate detection of genotypes of SNP (Single Nucleotide Polymorphism) sites is realized. Meanwhile, the zucchini whole genome 10K liquid-phase gene chip has the advantages of low cost and high efficiency, is beneficial to accelerating the breeding process of zucchini, and provides powerful technical support for development and utilization of zucchini germplasm resources.

A Cucurbita pepo subsp. pepo plant is provided. The Cucurbita pepo subsp. pepo plant comprises a genome having a loss of function mutation in a Cp4.1LG13g07780 gene or ortholog thereof, wherein the loss of function mutation confers a multiflowering (mf) trait. Also provided are methods of producing and using such plants.

A method of increasing skin firmness or elasticity of a person's skin is disclosed. The method can include topically applying a composition comprising an effective amount of a water extract of Cucurbita pepo (pumpkin) seed to skin in need thereof, wherein the water is a marine spring water and the Cucurbita pepo (pumpkin) seed extract comprises an amino acid from the Cucurbita pepo (pumpkin) seed, and wherein topical application of the composition increases skin firmness or elasticity of the person's skin.

The invention provides a cleome gynandra extract as well as a preparation method and application thereof, and belongs to the technical field of cleome gynandra extraction. The preparation method comprises the following steps: mixing cleome gynandra leaves with an extraction solvent, performing ultrasonic extraction, filtering and collecting filtrate, centrifugally collecting supernate, concentrating and freeze-drying to obtain the cleome gynandra extract, the extraction solvent is water, an ethanol solution with the volume fraction of 43-47% or an ethanol solution with the volume fraction of 88-92%. Research finds that the cleome gynandra extract extracted by using water, an ethanol solution with the volume fraction of 43-47% or an ethanol solution with the volume fraction of 88-92% has obvious DPPH and hydroxyl radical scavenging rate, the death rate and hatching rate of zebra fish can be increased, zebra fish nrf2 signal channel related genes are influenced, the oxidative stress degree is reduced, and the zebra fish nrf2 signal channel related genes can be inhibited. Further, an obvious protection effect on hydrogen peroxide induced oxidative stress zebrafish is achieved. The preparation method is simple, easy to operate and suitable for industrial production.

The utility model relates to the technical field of seed drying equipment, in particular to a summer squash seed drying chamber which comprises a bottom plate, side plates are fixedly connected to the two side ends of the bottom plate, a back plate and a face plate are fixedly connected to the front ends and the rear ends of the side plates respectively, a cover plate is arranged at the upper ends of the side plates, and drying mechanisms are arranged between the side plates and on the side walls of the side plates. A discharging mechanism is fixedly connected to the upper surface of the cover plate, and a PLC used for controlling the drying mechanism and the discharging mechanism is arranged on the outer wall of the side plate. The PLC is in linkage with the capacitive moisture sensor and the temperature and humidity sensor, the moisture content of seeds and drying environment parameters are monitored in real time, the temperature and the air speed of the air heater and the running speed of the conveying belt a and the conveying belt b are dynamically adjusted, it is ensured that the drying process is accurate and controllable, and excessive drying or energy waste is avoided. And secondly, opening and closing of the turning plate and the discharging speed are automatically adjusted according to the initial moisture content data, continuous and intelligent operation is achieved, and the manual intervention requirement is remarkably reduced.

This utility model discloses a device for identifying salt tolerance during the germination period of pumpkin seeds, belonging to the technical field of seed salt tolerance identification equipment. The utility model includes an incubator, with a tray inside the incubator's cavity. A flat support plate is located inside the tray's cavity. A circular hole is formed at the top of the flat support plate, and adjustment components are provided on both sides of the flat support plate. By pulling out the tray, different concentrations of salt solution can be injected into the cavities of the three trays, enabling simultaneous comparative experiments with multiple groups. The circular hole design facilitates the placement of pumpkin seeds, and the salt solution enters through a drainage hole, ensuring full contact between the seeds and the solution. Pulling the sliding frame moves the locking plate out of the locking groove, allowing the flat support plate to be moved up and down to adjust the depth of seed immersion in the salt solution. After adjustment, the sliding frame is pushed back to fix the plate, achieving precise control of the seed immersion depth and providing convenience for studying the effects of different salt concentrations and immersion depths on seed germination.

The invention belongs to the technical field of vegetable quality character molecular marker development and molecular marker-assisted breeding, relates to a KASP molecular marker for American pumpkin peel lignin characters and application of the KASP molecular marker, and particularly relates to rapid screening and molecular marker-assisted breeding of American pumpkin peel lignin. The KASP molecular marker is an SNP (Single Nucleotide Polymorphism) marker and is positioned at the 8697115 bp position of a No.3 chromosome of the American pumpkin; the KASP molecular marker CpPL (Peel lignin) is closely linked with the characters of the American pumpkin peel lignin, and the molecular marker can be used for identifying and screening whether the peel lignin of different American pumpkin materials exists or not and assisting in selective breeding of offspring of the American pumpkin materials.

The invention provides a method for establishing an American pumpkin genetic transformation system. The method comprises the following steps: firstly, culturing to obtain an American pumpkin explant; carrying out dip dyeing on the explant by taking positive agrobacterium, and culturing the dip-dyed explant on a co-culture medium; washing the explant with sterile water, and transferring the explant to a recovery culture medium for culture; transferring the explants to a screening culture medium for culturing; and finally, transferring the explants to a screening rooting culture medium, and transplanting and planting seedlings after the explants are cultured into the seedlings, so as to obtain American pumpkin genetic transformation plants. Technical improvement is carried out from three dimensions of a regeneration system, a screening system and a rooting system to successfully obtain the first American pumpkin transgenic positive seedling in China; the genetic transformation efficiency is improved to 2.5% and is about 3 times that reported before, and the genetic transformation system of the American pumpkins is further improved.

The present invention provides a lubricant for treating vaginal dryness, maintaining vaginal health, and to improve libido. Specifically, the invention discloses a herbal vaginal lubricant composition comprising an extract of Cucurbita pepo (Pumpkin) seed, aqueous extract of Abelmoschus esculentus (Lady's Finger / Okra), Cucurbita pepo (Pumpkin) seed oil, Shea Butter, Hippophae rhamnoides (Sea Buckthorn) oil and pharmaceutically acceptable excipients, wherein, the herbal vaginal lubricant is an oil-in-water emulsion gel.

The utility model relates to the technical field of cucurbita pepo planting, and particularly discloses a pest control device for cucurbita pepo planting, which comprises a base, supporting legs arranged at the bottom of the base and provided with universal wheels, a storage battery box arranged at the bottom of the base, and a traction frame arranged on the left side of the base, a pesticide mixing mechanism and a spraying mechanism for pest control are arranged at the top of the base; the medicine mixing mechanism comprises a water tank fixedly installed at the top of the base, a fixed cylinder fixedly installed at the top of the base through supporting legs and a mixing box, a first metering pump fixedly installed at the top of the base is arranged on the outer side of the water tank, and independent and accurate metering of clear water and various medicine liquid is achieved through combination of the first metering pump and a second metering pump; and in the pesticide liquid spraying process, clear water and pesticide liquid can be conveyed according to the preset proportion, continuous operation can be achieved without manual intervention, the problem that a traditional device needs to be shut down frequently for pesticide supplementing is solved, and operation continuity is improved.

The application discloses a kind of cucurbita pepo powdery mildew resistance related molecular markers and its KASP primer combination and application, belong to plant resistance breeding technical field.The mutation site of the cucurbita pepo powdery mildew resistance related molecular marker is located on the 11th chromosome of cucurbita pepo chromosome, and the mutation of nucleotide G→C at the 24091441th position;The application of the described cucurbita pepo powdery mildew resistance related molecular marker in cucurbita pepo powdery mildew resistance material screening or cucurbita pepo powdery mildew resistance breeding;The KASP primer combination for detecting the cucurbita pepo powdery mildew resistance related molecular marker is disclosed in the application, and the sequence is as follows: the upstream primer combined with the disease resistance site is as shown in SEQ ID NO.1;The upstream primer combined with the susceptible site is as shown in SEQ ID NO.2;The downstream primer is as shown in SEQ ID NO.3;The cucurbita pepo powdery mildew resistance related molecular marker and its KASP primer combination of the application can significantly distinguish cucurbita pepo powdery mildew disease resistance material and cucurbita pepo powdery mildew susceptible material, and can be applied in cucurbita pepo powdery mildew resistance material screening or cucurbita pepo powdery mildew resistance breeding.

This invention belongs to the field of molecular marker development for vegetable quality traits and marker-assisted breeding technology, specifically relating to rapid screening for the presence or absence of seed coat in squash and marker-assisted breeding. This invention discloses a KASP molecular marker for detecting the presence or absence of seed coat in squash and its application. This molecular marker, CpSC (Seed coat), is closely linked to the absence of seed coat in squash and can be used for the identification and selection-assisted breeding of seed coat traits in different squash materials and their progeny.

The utility model relates to the technical field of seed cleaning equipment, in particular to zucchini seed piece cleaning equipment which comprises a base plate, two fixed supports and two oil cylinders, the two fixed supports are fixedly connected to the upper surface of the base plate, the fixed ends of the oil cylinders are located on one sides of the fixed supports and connected to the side end of the base plate in a rotating mode, and the oil cylinders are connected to the fixed ends of the oil cylinders in a rotating mode. The upper ends of the fixed support and the oil cylinder are connected with a bearing frame, and the bearing frame is rotationally connected with a cleaning assembly. By arranging the cleaning assembly, during working, a water pump of the cleaning assembly supplies water to a water spraying pipe located in the cleaning roller, the water spraying pipe can clean summer squash seeds at the bottom of the inner wall of the rotating cleaning roller through water sprayed out of a drainage hole, and the summer squash seeds can be cleaned at a time; the defects in the prior art are overcome.

The utility model discloses a separated type cucurbita pepo inserting needle rotary dicing table mechanism, relates to the technical field of food processing equipment, and solves the problems that equipment for transferring cucurbita pepo to different stations occupies a large space and is low in automation efficiency. Three different stations of feeding, dicing and waste discharging can be respectively connected, the three different stations of feeding, dicing and waste discharging are efficiently connected and integrated together through multi-station multifunctional rotary progressive matching and reasonable utilization of limited space, the overall function of the pumpkin dicer is achieved through mutual matching, the overall structure is simple and independent, and the operation is convenient. According to the invention, the requirement on the manufacturing precision of parts is low, the assembly manufacturability is good, the main actions of the whole machine adopt pneumatic control production, the response speed is high, the operation is simple and convenient, the action control of the production process is easy to realize, the action is accurate, the positioning precision is high, and the functions of energy conservation and environmental protection are achieved while the whole action requirement can be efficiently completed.

The invention belongs to the technical field of biology, and particularly relates to application of CpWRKY51 in regulation of powdery mildew resistance of cucurbita pepo. The nucleotide sequence of the CpWRKY51 is as shown in SEQ ID NO.1. The invention finds that the CpWRKY51 enhances the resistance to powdery mildew by triggering strong allergic reaction and cell death and up-regulating antioxidant enzyme to relieve oxidative stress, so that the application of the CpWRKY51 in regulating the resistance of summer squashes to powdery mildew is provided.

The invention relates to the technical field of biology, in particular to application of CpSnRK2.6 to regulation and control of stress resistance of zucchini. It is found that CpSnRK2.6 can be induced by drought and salt stress, the transcriptional level of CpSnRK2.6 is rapidly increased after abscisic acid treatment, an overexpression plant is obtained by constructing an overexpression vector, a non-overexpressed plant has remarkable yellow leaves and wilting, a CpSnRK2.6 overexpressed plant only shows slight wilting symptoms, and the CpSnRK2.6 overexpressed plant has a good application prospect. The CpSnRK2.6 overexpressed plant relieves the leaf water loss and withering of the non-overexpressed plant, and after the CpSnRK2.6 overexpressed plant is rehydrated for 3 days, the CpSnRK2.6 overexpressed plant recovers normal growth, and the non-overexpressed plant cannot recover, so that the application of the CpSnRK2.6 in regulating and controlling the stress resistance of the zucchini is provided.

The invention provides a pharmaceutical composition for resisting inflammation and repairing oral ulcer and a preparation method thereof, and belongs to the technical field of biological pharmacy, and the pharmaceutical composition comprises ciona glabra peptide, a cucurbita pepo seed extract, carboxymethylation modified hemerocallis citrina root polysaccharide, honeysuckle chlorogenic acid, baicalin and dipotassium glycyrrhizinate. The ciona ciona peptide is prepared by carrying out step-by-step enzymolysis on ciona ciona through trypsin and lumbrukinase and purifying through a sephadex G-15 column. The cucurbita pepo seed extract is prepared by breaking walls of cucurbita pepo seeds with a compound enzyme, performing enzymolysis with ficin protease and subtilisin, and purifying with a polyamide resin column. The carboxymethylation modified hemerocallis citrina root polysaccharide is prepared by alkalization and chloroacetic acid modification of hemerocallis citrina root extracted polysaccharide. All the components are compounded for use, so that multiple effects of resisting inflammation, easing pain and promoting epithelial cell proliferation and mucosal barrier repair are synergistically realized, and the treatment effect of oral ulcer is optimized.

The utility model relates to the technical field of cucurbita pepo breeding, in particular to a cucurbita pepo breeding device which comprises a device body, a small motor is fixedly connected to the top in the device body through a fixing plate, a fan is fixedly connected to the driving position of the small motor, and a main water pipe is fixedly connected to the right side of the upper portion in the device body. According to the cucurbita pepo breeding device, the cucurbita pepo disinfection and germination accelerating device is installed, the whole process from disinfection to germination accelerating can be completed through one device, the working efficiency is high, the operation is simple, the device is convenient to operate, the cost is low, and the device is suitable for large-scale popularization and application. The stirring device is installed to promote uniform mixing of the seeds and the liquid medicine and improve the disinfection efficiency, and meanwhile, a temperature sensor, a heating rod, a fan and a dropper are installed to enable the zucchini seeds to keep certain humidity and temperature, so that the budding rate of the zucchini seeds is increased.

A method of reducing the appearance of fine lines or wrinkles on a person's skin is disclosed. The method can include topically applying a composition comprising an effective amount of an aqueous extract of Cucurbita pepo (pumpkin) seed to the fine lines or wrinkles present on the person's skin. Topical application of the composition can reduce the appearance of the fine lines or wrinkles on the person's skin.