Method for selecting and breeding cabbage type rape cytoplasm sterile line through rape doubled haploid inducible system

A Brassica napus, double haploid technology is applied in the directions of horticultural methods, botanical equipment and methods, plant genetic improvement, etc., and can solve the problems of low induction efficiency and the like

Active Publication Date: 2016-11-09
CHENGDU ACAD OF AGRI & FORESTRY SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

The earliest maize haploid induction line is stock6, which can only induce haploids in maize, and then the haploid plants undergo artificial chromosome doubling to form homozygous diploids (double haploids), and the induction efficiency Low, generally the induction efficiency is below 10% (calculated based on the number of haploids obtained from harvested seeds)

Method used

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  • Method for selecting and breeding cabbage type rape cytoplasm sterile line through rape doubled haploid inducible system
  • Method for selecting and breeding cabbage type rape cytoplasm sterile line through rape doubled haploid inducible system
  • Method for selecting and breeding cabbage type rape cytoplasm sterile line through rape doubled haploid inducible system

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Experimental program
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Effect test

Embodiment 1

[0079] see figure 1 , figure 2 , Figure 4 , Figure 5 , Figure 7 , Brassica napus microspore cultured progeny DH0051 in vitro was testcrossed with the Polyma cytoplasmic male sterile line Rong A0068, and the testcross offspring were identified, and the testcross offspring were highly sterile. The maintainer of the sterile line. The pollen of rapeseed double haploid induction line Y3560 was used to pollinate the sterile single plant of the test cross progeny, bagged and isolated, and the seeds of the induced progeny were harvested. The induced offspring were planted, and the ploidy of the induced offspring was identified by flow cytometry at the seedling stage, and the polyploid, haploid, and aneuploid plants were eliminated. At the flowering stage, eliminate the sterile plants with dominant traits (dwarf traits) of rapeseed double haploid induction line Y3560 and select 15 sterile individual plants that are completely sterile and have better plant growth. The inducib...

Embodiment 2

[0157] see figure 1 , figure 2 , Figure 4 , Figure 5 , Figure 8 , Brassica napus microspore cultured progeny DH0034 in vitro was testcrossed with radish cytoplasmic sterile line Rongluo A100, and the offspring of the testcross were identified and found to be highly sterile. The maintenance line of the breeding line. The pollen of rapeseed double haploid induction line Y3380 was used to pollinate the sterile single plant of test cross progeny, bagged and isolated, and the seeds of induced progeny were harvested. The induced offspring were planted, and the ploidy of the induced offspring was identified by flow cytometry at the seedling stage, and the polyploid, haploid, and aneuploid plants were eliminated. At the flowering stage, the sterile plants with dominant traits (dwarf traits) of rapeseed double haploid induction line Y3380 were eliminated. 20 sterile individual plants with complete sterility and good plant growth were selected, and the sterile individual plan...

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Abstract

The invention provides a method for selecting and breeding a cabbage type rape cytoplasm sterile line through a rape doubled haploid inducible system. The method comprises the following steps: 1), determining restoring and maintaining relationships of stable strains; 2), according to test-crossing progeny trait specialization, performing backcross or multi-generation backcross on a temporary maintainer line and a stable sterile line; 3), pollinating the backcross progeny by using the rape doubled haploid inducible system; 4), identifying fertility of induced progeny; 5), selecting a perfect sterile single strain, and continuously using the rape doubled haploid inducible system to pollinate the sterile single strain; 6), after twice inducible pollinated progeny forms the sterile line, identifying the genetic stability and the sterility of the strain; 7), using the rape doubled haploid inducible system to pollinate, maintain, and select and breed stable new sterile lines with the maintainer line characteristics. According to the method provided by the invention, the cabbage type rape cytoplasm sterile line can be quickly selected and bred, and four generations can be obtained within two years at most; the genetic-stability cabbage type rape cytoplasm sterile line is obtained, the selecting and breeding speed of the cabbage type rape cytoplasm sterile line is greatly improved, the selecting and breeding speed and efficiency of the cabbage type rape hybrid variety are improved, and the labor and material investments are reduced.

Description

Technical field: [0001] The invention relates to agriculture, in particular to a method for breeding a cytoplasmic sterile line of Brassica napus double haploid induction line. Background technique: [0002] Rapeseed is the main oilseed crop in my country, of which Brassica napus accounts for more than 90% of the cultivated varieties of rapeseed. Brassica napus ( Brassica napus , a compound species evolved from Brassica oleracea (aa, n=10) and Brassica cabbage (cc, n=9) through natural interspecific hybridization and double diploidization. According to the source of chromosomes, it is tetraploid, 2n=38 ). Currently, more than 90% of Brassica napus cultivars are hybrids. The utilization of heterosis in Brassica napus originated from the discovery of the polima CMS cytoplasmic sterile line by Academician Fu Tingdong of Huazhong Agricultural University. Since the 1990s, cytoplasmic sterility has accounted for more than 90% of heterosis utilization in Brassica napus. In rec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H4/00
CPCA01H1/02A01H1/04A01H4/001A01H4/008
Inventor 付绍红杨进王继胜李云邹琼陶兰蓉康泽明唐蓉
Owner CHENGDU ACAD OF AGRI & FORESTRY SCI
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