Isaria fumosorosea wettable powder as well as preparation method and application thereof
A technology of I. fumigatus and wettable powder, applied in the field of wettable powder of I. fumigatus and its preparation, can solve the problem that the control effect is greatly affected by environmental conditions, the control effect is unstable, and the adhesion is not good. and other problems, to achieve the effect of good application prospects, excellent performance indicators, and good control effect
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Embodiment 1
[0045] Example 1 The preparation of rose fume-colored I. spp. conidia powder
[0046] The I. fumigatus used in the examples of the present invention is I. fumigatus strain SCAU-IFCF02, which was deposited in the China Center for Type Culture Collection on November 1, 2013, with a preservation number of CCTCC NO: M 2013526.
[0047] The preparation steps of the rose fume-colored clavula conidia powder of the present invention are as follows:
[0048] S1. First-level solid culture, take 50uL of the original bacterial liquid of I. fumigatus strain SCAU-IFCF02 and spread it on the SDAY solid medium (SDAY medium is the most suitable medium), and cultivate it at 24°C, the best culture The time is 15 days;
[0049] S2. Secondary liquid shaking culture, wash the spores on the SDAY solid medium with sterile water containing 0.03% Tween-80, and then filter with gauze to make 1×10 7 1 spore / mL of spore suspension, 100uL of the above spore suspension was inoculated into 200mL of Sabou...
Embodiment 2
[0057] Example 2 Optimal experiment of wetting agent for I. fumoscens wettable powder
[0058] 1. Test material
[0059] (1) The conidia powder prepared in Example 1.
[0060] (2) In this embodiment, the wetting agents involved in the present invention cannot be repeated here one by one, and the typical wetting agents summarized in the following several experimental processes are selected for illustration: SDBS (sodium dodecylbenzene sulfonate) ), SDS (sodium dodecyl sulfate), OP-10 (octylphenol polyoxyethylene ether-10), AEO-9 (fatty alcohol polyoxyethylene ether), penetrating agent T (Penetrating agent T) and penetrating agent agent JFC.
[0061] 2. Experimental method
[0062] (1) Determination of wetting force: Add each wetting agent into talcum powder at the ratio of 1%, 3% and 5%, and 10% of the rose smoke-colored I. Samples with different aerosol content but same spore content. The wetting time is measured according to the standard method of GB / T 5451-2001, th...
Embodiment 3
[0072] Example 3 Dispersant Optimum Experiment of I. fumoscens Wettable Powder
[0073] 1. Test material
[0074] (1) The conidia powder prepared in Example 1.
[0075] (2) In this embodiment, the dispersants involved in the present invention cannot be repeated here one by one, and the typical dispersants summarized in the following several experimental processes are selected for illustration: Sodium methylene dinaphthalene sulfonate (Diffusion promoter NNO ), sodium lignosulfonate (Sodium lignosulfonate), sodium polyacrylate (Sodium polyacrylate), dispersant MF (Diffusion promoter MF), dispersant P-19 and polyethylene glycol (Polyethylene glycol).
[0076] 2. Experimental method
[0077] (1) Determination of dispersibility: The screening of dispersants includes two processes of coarse sieving and fine sieving. Coarse sieving is that each dispersant is mixed with a sample containing 3% wetting agent OP-10, 10% spore powder, and 82% talcum powder at a ratio of 5% (w / w)...
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