Method based on genome size and for improving cruciferous crop characters

A cruciferous crop, genome technology, applied in the direction of plant genetic improvement, botanical equipment and methods, applications, etc.

Pending Publication Date: 2020-01-17
CHENGDU ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide a method for improving the traits of cruciferous crops based on the size of the genome, so as to obtain excellent parents of cruciferous crops, and to solve the problem of strong dominant combination in the process of cruciferous crop heterosis utilization For the selection and improvement of parents, there is no need for continuous self-crossing of parents for multiple generations, let alone continuous backcrossing of stable parents for multiple generations, so as to select and breed parents with higher and better traits.

Method used

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  • Method based on genome size and for improving cruciferous crop characters
  • Method based on genome size and for improving cruciferous crop characters
  • Method based on genome size and for improving cruciferous crop characters

Examples

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Embodiment 1

[0058] see figure 1 with image 3 This example provides a method for improving the traits of cabbage based on the size of the genome. The purpose of the improvement is to quickly obtain several homozygous varieties of cabbage with outstanding comprehensive traits such as strong disease resistance, high yield, and storage and transportation resistance, or a single trait as parents. Further improve the utilization rate of cabbage heterosis. Through the collection, identification and genome size determination of germplasm resources of cabbage, a homozygous line of cabbage with disease resistance (black rot resistance and soft rot resistance) and high yield was selected with a relative difference percentage T of genome size T of 8%. F 1 generation seeds.

[0059] Choose the right sowing time to sow F 1 Generation, make F 1 The generation meets the flowering stage of the double haploid induced line of rapeseed, and the F 1 After artificial emasculation, the rapeseed double ha...

Embodiment 2

[0079] see Image 6 , this example provides a method for improving the traits of Brassica napus based on the size of the genome. The purpose of the improvement is to quickly obtain a number of homozygous Brassica napus varieties with outstanding comprehensive or single traits such as disease resistance, high yield, lodging resistance, and high oil content. Further improve the utilization rate of heterosis in Brassica napus. Through the collection, identification and genome size determination of the germplasm resources of Brassica napus, the clubroot-resistant, high-yield multi-year self-bred homozygous line ZH102 and the lodging-resistant, oily A homozygous line 3581 (oil content > 56%) with a high amount of self-breeding for many years was crossed after emasculation to obtain F 1 generation seeds.

[0080] Choose the right sowing time to sow F 1 Generation, make F 1 The generation meets the flowering stage of the double haploid induced line of rapeseed, and the F 1 After...

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Abstract

The invention discloses a method based on genome size and for improving cruciferous crop characters. The method includes: S1, collecting germplasm resources of cruciferous crops, and measuring genomesize E; S2, subjecting genome size relative difference percent T to reciprocal crossing of 2-20% cruciferous crops to obtain F1 generation; S3, planting the F1 generation, and performing artificial castration; S4, using a rape dihaploid inducer to induce the F1 generation to obtain a Y1F2 generation; S5, planting the Y1F2 generation, selecting orderly single plants with normal fertility and ploidyfor selfing to obtain a Y1F3 generation; S6, planting the Y1F3 generation, identifying its stability, and analyzing normal distribution condition of genome size Eyx of the Y1F3 generation; S7, investigating target character of the Y1F3 generation; S8, selecting a strain with the target character and Eyx positioned within 3-5% of two ends of normal distribution as an improved breeding new material. The method has the advantages of short improving period, high efficiency and low cost.

Description

technical field [0001] The invention belongs to the technical field of crop breeding and relates to a method for improving the traits of cruciferous crops according to the genome size. Background technique [0002] Cruciferous crops include Chinese cabbage, pak choy, Chinese cabbage, kale, seaweed stalk, wutah, turnip, floret, green cabbage, cabbage type rape, head cabbage (cabbage), collard greens, bush cabbage, Brussels sprouts Cabbage, kohlrabi, kale, cauliflower (cauliflower), broccoli, kohlrabi, Brassica napus, mustard greens, mustard mustard, mustard rape, radish and other crops are common ingredients on the table because of their high dietary Features such as fiber, low calorie, and rich nutrients provide people with high-quality nutrition. At the same time, rapeseed among cruciferous crops is also a major oil crop, and its seeds are an important source of edible oil, providing people with rich polyunsaturated fatty acids. It can be seen that cruciferous crops have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 付绍红朱振东殷丽琴李云杨进王继胜邹琼康泽明陶兰蓉唐蓉
Owner CHENGDU ACAD OF AGRI & FORESTRY SCI
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