8 results about "Genome size" patented technology

The present invention relates to a viral expression backbone, viral expression vectors produced therefrom, and pharmaceutical compositions thereof, which are suitable for therapeutic or in vitro applications, as well as methods related thereto. In other words, the genome size of the modified HSV-1 vector generated by the HSV-1 precursor vector provided by the invention is equivalent to that of a wild type HSV-1 genome.

This invention belongs to the field of gene technology and discloses a bacterial strain B11 antagonistic to soft rot disease of Amorphophallus muelleri. Isolated from the rhizosphere soil of Amorphophallus muelleri, it was identified as the golden subspecies of *Pseudomonas aeruginosa*. This strain effectively inhibits the growth of the soft rot pathogen Pcc, reducing the incidence and disease index of soft rot in Amorphophallus muelleri. It also possesses the ability to dissolve organophosphates and produce siderophores, and exhibits broad-spectrum antagonistic activity against various fungal diseases of Amorphophallus muelleri, including white mold, leaf spot, and dry rot. Whole-genome analysis shows that the B11 genome is 6.77 Mb in size, with a GC content of 62.81%, encoding 5,964 genes, including gene clusters related to antibacterial activity such as phenazine, nitropyrrolizin, and siderophores. This strain has good development and application potential in the control of soft rot in Amorphophallus muelleri.

The invention discloses a giant strong aeromonas hydrophila bacteriophage and application thereof, and belongs to the technical field of microorganisms. According to the invention, aeromonas hydrophila is taken as host bacteria, the phage is enriched from a collected lake water sample, and is separated and purified by adopting a double-layer agar plate method, so that a strain of phage is successfully obtained, the strain of phage is named as aeromonas hydrophila phage Cgtyf, the preservation number is CCTCC NO: M 2026099, and the titer of the phage can reach 1.0 * 10 < 10 > PFU / mL; the genome size of the phage is about 237 kb, and the phage belongs to a giant phage. In an in-vitro bacteriostasis experiment, the bacteriophage Cgtyf shows a remarkable bactericidal effect and a relatively strong growth inhibition capability on host bacteria, and shows a relatively high temperature tolerance range in a temperature stability test. Therefore, the bacteriophage Cgtyf provided by the invention can be effectively used for preventing and controlling aeromonas hydrophila in aquatic products, has a potential application value as an antibacterial drug substitute, is beneficial to reducing or avoiding the use of antibacterial drugs in aquaculture, and has a good application prospect.

The invention discloses a K4 subcluster mycobacterium bacteriophage, a separation method and application thereof, Msm is used as a host to separate and purify a new bacteriophage K4SD6 from soil, the genome size of the new bacteriophage K4SD6 is 59.028 kb, the bacteriophage shows good lysis activity on mycobacterium tuberculosis H37Ra, and a mouse infection model verifies that the new bacteriophage K4SD6 has a good anti-tumor activity on mycobacterium tuberculosis H37Ra. The K4SD6 bacteriophage has an extremely strong capability of removing H37Ra in a mouse body, and the K4SD6 bacteriophage can be used for efficiently removing the H37Ra infected in the mouse body.

This invention discloses a method and its application for identifying key functional genes of biological phenotypes based on cross-species gene sequence expression characteristics. The method includes: obtaining a reference genome of a small number of target organisms and classifying them into orthologous gene groups; obtaining raw transcriptome data of tissues of interest or specific phenotypes for each target species, cleaning and aligning them to the reference genome to obtain a gene expression matrix; constructing a feature value dataset by combining temporary sequence numbers and expression levels for each subgroup of transcriptome samples; obtaining phenotypic feature information for each corresponding sample and constructing a machine learning prediction model; and determining the influence weight of each subgroup of total sequence expression based on the optimal model, thereby locating and obtaining key functional genes for specific phenotypes. This invention achieves a wider range of applications with a small requirement for species genome size, and has promising application prospects.

A CE based method and kit for the determination of the size and purity of an AAV genome which relies on Capillary Electrophoresis-Laser Induced Fluorescence (CE-LIF) analysis. These methods and kits are capable of detecting intact and partial genomes in a virus vectors such as adeno-associated viruses as well as remove small size impurities. In one example, the method can include creating a nucleic acid ladder using CE-LIF, releasing the genome from within an adeno-associated virus, purifying said genome and analyzing said genome using CE-LIF and comparing the results of the analysis of the genome to the nucleic acid ladder to determine a size of nucleic acids in the genome.

The present disclosure relates to using size exclusion chromatography to isolate AAV genome, to determine the vector genome size purity of a composition comprising isolated rAAV particles, to assess the folding or secondary structure of vector genomes inside the capsids, and to determine vector genome titer (Vg) of a composition comprising isolated rAAV particles.

This invention discloses a universal method for preparing nuclear suspensions suitable for flow cytometry of Rosa species, belonging to the field of plant biotechnology. The method uses WPB dissociation buffer and follows a standardized procedure to process Rosa leaves in different storage states through sample dissociation, filtration, staining, and incubation steps: leaf tissue is dissociated using WPB dissociation buffer pre-cooled to 4°C, allowed to stand on ice, filtered through a 40µm cell filter, and brought to volume. Propidium iodide and ribonuclease A are added for staining and incubation before flow cytometry analysis. This invention is directly applicable to Rosa leaves in three storage states: fresh, silica gel dried, and -80°C frozen. It is simple to operate, highly versatile, and the prepared nuclear suspension yields high-quality peak histograms with low coefficient of variation when analyzed by flow cytometry, providing an efficient and standardized technical solution for large-scale ploidy analysis and genome size determination of Rosa species.