Space methylobacterium LCT-S10-2

A Methylobacter, space technology, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as difficulty in obtaining genetic information

Inactive Publication Date: 2016-11-09
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Early people's understanding of many environmental microorganisms only came from the study of 16S rRNA, and some important genetic information is difficult to obtain

Method used

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  • Space methylobacterium LCT-S10-2
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  • Space methylobacterium LCT-S10-2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Implementation example one: strain LCT-S10-2 culture.

[0026] The strain LCT-S10-2 was isolated from the condensed water in the return cabin of the "Shenzhou 9" spacecraft. strains were

[0027] Static or shaking culture in an incubator or shaker at 37°C, and the liquid medium is brain heart extract medium

[0028] (BHI, Oxiod), the formula is 37 g / L BHI; the solid medium is MH plate.

Embodiment 2

[0029] Implementation example two: strain LCT-S10-2 strain identification.

[0030] Strain LCT-S10-2 was inoculated into BHI liquid medium, cultured overnight at 37°C, 180 rpm, and centrifuged at 6000 rpm.

[0031] Incubate for 5 min to collect the cells and discard the supernatant. The genomic DNA of the collected bacteria was extracted using the genomic DNA extraction kit of Qiagen (Code No. 51304, see the product manual for detailed steps). Using the genomic DNA of LCT-S10-2 as a template, the bacterial 16s rRNA general sequencing primers 27F (5'- AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACGGCTACCTTGTTACGACTT-3') were used to amplify and sequence the 16s rRNA fragment, and obtained its base

[0032] base sequence.

Embodiment 3

[0033] Implementation example three: Gram staining of bacterial strain LCT-S10-2.

[0034] LCT-S10-2 cells were stained with Gram staining kit (Code No. G1060, Solarbio).

[0035] Cultivate the bacteria overnight in BHI liquid medium, drop the bacteria solution on the center of the glass slide, and warm it slightly on the alcohol lamp to make it dry quickly. Crystal violet and sand yellow staining solution can be used to stain and decolorize the dried bacteria at one time. For detailed steps, please refer to the product manual. After the staining was completed and dried, the staining was observed under an optical microscope.

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Abstract

The invention relates to a strain of downward strain space methylobacterium LCT-S10-2 separated out of a re-entry capsule of the Shenzhou IX airship. The space methylobacterium LCT-S10-2 is an aerobic bacterium, a short-rod-shaped gram stain variable strain (figure 1), a chemoheterotrophic bacterium, a facultative methylotrophic bacterium and a rare facultative methane nutritional bacterium, and the strain grows slowly. The space methyl bacillus LCT-S10-2 is subjected to 16S rRNA sequencing, and is identified as a methylobacterium member. Whole genome sequencing analysis is carried out, the whole genome size is 4.57 Mbp, the GC content is 66.84%, the predicted gene number is 4946, and protein of LCT-S10-2 is classified and annotated as 20 kinds of COG families. The LCT-S10-2 bacterium can grow on the surface of epoxy resin and the surface of vulcanized natural rubber, and a corrosive biological film is generated. The bacterium cannot grow on the surface of ester polyurethane, the surface of ether polyurethane and the surface of polyvinyl formal, and no corrosive biological film is generated. Certain help is provided for studies of species distribution of microorganisms in an airtight aircraft and selection of corrosion-resistant materials.

Description

technical field [0001] The invention belongs to the field of biotechnology; more specifically, the invention relates to Methylobacterium space LCT-S10-2, its biological characteristics, and whole genome sequencing. Background technique [0002] With the development of aerospace technology, human exploration activities in outer space are becoming more and more frequent. Microorganisms are found almost everywhere on the ground, most of which are found on the human skin, oral cavity, nasopharynx and gastrointestinal tract. Although the manned spacecraft and its internal items have undergone strict vacuum disinfection, microorganisms such as bacteria and fungi are still easy to breed in the airtight environment of manned spacecraft, and these microorganisms can form biofilms on the surfaces of metals and polymer composite materials in airtight cabins , their growth, reproduction and metabolism will corrode materials, which seriously threatens the long-term on-orbit operation sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/205C12R2001/01
Inventor 刘长庭徐绸张学林周宏方向群姜学革刘岩潘磊黄兵李佳余昳
Owner GENERAL HOSPITAL OF PLA
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