Method for quickly identifying genome size of sweet potatoes and application thereof

A genome and sweet potato technology, applied in the field of plant ploidy detection, can solve the problems of sweet potato genome size controversy, poor detection results, and failure to meet the accuracy requirements of sweet potato genome
CN109596502AActive Publication Date: 2019-04-09XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
Publication Date
2019-04-09

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Abstract

The invention provides a method for quickly identifying the genome size of sweet potatoes, belonging to the technical field of plant ploidy detection. The method comprises the steps of 1) taking sweetpotato tips from a field and performing cottage on the sweet potato tips in aHoagland nutrient solution for culturing for 20-28h; 2) mixing unexpanded leaves or new root tips with a lysis solution special for sweet potatoes for tissue lysis to obtain liquor produced after lysis; 3) filtering the liquor produced after lysis, collecting the filtrate, centrifuging, and collecting the centrifugationproduct as lysed cells; 4) mixing the lysed cells with a dye solution for dyeing, and obtaining a to-be-detected cell solution by vortex oscillation; 5) detecting the FL2-A value of the to-be-detectedcell by a flow cytometer; and 6) calculating to obtain the 1C value of the genome of the to-be-detected cell and the genome size of the to-be-detected cell according to the FL2-A value of the to-be-detected cell. The method can also identify the genome size of other species of ipomoea, and provide a fast, efficient and low-cost determination method for identifying the genome of sweet potatoes andrelated wild species.
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Description

technical field

[0001] The invention belongs to the technical field of plant ploidy detection, and in particular relates to a method for quickly identifying the genome size of sweet potato and its application. Background technique

[0002] In recent years, with the vigorous development of flow cytometry (FCM), it can be used for immunophenotyping, cell counting, cytokine analysis, microsphere-based immunoassay, platelets, stem cells, apoptosis, cell cycle , DNA damage and proliferation, cancer research, cell signaling, calcium efflux, gene expression, kinetics, microbial enumeration and activity, water quality analysis, aquatic biology research, microbial species identification, bioprocessing, biofuels, plant biology, food science , veterinary medicine and other fields. In botany, flow cytometry is mainly used for the determination of nuclear DNA content, DNA ploidy identification, and cell cycle analysis.

[0003] Flow cytometry (Flow cytometry, FCM) has been applied to t...

Claims

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