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Method for fast cultivating abies beshanzuensis seedlings through embryo rescue technology

An embryo rescue and technology technology, applied in the field of rapid cultivation of Baishanzu fir seedlings, can solve the problems of ecological environment impact, weak sustainable reproduction ability, difficulty in natural regeneration, etc., and achieve the effect of strong operability and low pollution rate.

Active Publication Date: 2018-09-21
ZHEJIANG UNIV
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Problems solved by technology

At the same time, Baishanzu fir is affected by the ecological environment, which causes problems such as difficulty in natural regeneration and extremely weak sustainable reproduction ability.

Method used

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  • Method for fast cultivating abies beshanzuensis seedlings through embryo rescue technology
  • Method for fast cultivating abies beshanzuensis seedlings through embryo rescue technology
  • Method for fast cultivating abies beshanzuensis seedlings through embryo rescue technology

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Embodiment 1

[0024] Materials: In this example, immature embryos and endosperms of Abies baishanzu were used as explants for culture.

[0025] Step (1): Collect materials

[0026] From late July to early August, the immature cones of Baishanzu fir were collected in Baishanzu Nature Reserve. The color is green. The surface scales and bract scales of this green immature fruit are closely arranged. It can be placed in a sealed bag and stored at a low temperature of 4°C for later use.

[0027] Step (2): Seed Surface Sterilization

[0028] Separate the seed scales and seeds with tweezers, wash the seeds under running water for 3 hours, treat them with 70% ethanol for 30 seconds on an ultra-clean workbench, and rinse them with sterile water for 3 to 5 times. After treating with 0.1wt% mercuric chloride for 10 minutes, rinse with sterile water for 3 to 5 times and dry the surface moisture with sterile filter paper.

[0029] Step (3): Immature embryo isolation and inoculation

[0030] Place th...

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Abstract

The invention discloses a method for fast cultivating abies beshanzuensis seedlings through the embryo rescue technology, and aims at solving the problem that abies beshanzuensis is critically endangered. According to the method, immature cones of wild plants of the abies beshanzuensis in the Baishanzu nature reserve are adopted as starting materials, immature embryos and endosperm of the immaturecones are picked off after surface disinfection is carried out, wild plant offsprings of the abies beshanzuensis are fast cultivated through the embryo rescue technology, and a large number of abiesbeshanzuensis seedlings are obtained within a short time. The method has the advantages that the immature embryos of the abies beshanzuensis are utilized, after cultivation is carried out for 30 days,many abies beshanzuensis seedlings are obtained, the cultivation speed of the abies beshanzuensis seedlings is increased, and the current situation that the abies beshanzuensis is critically endangered is relieved. The method is high in operability, simple, efficient and low in pollution rate, and has important significance on fast cultivation of threatened plant seedlings in the world.

Description

technical field [0001] The invention relates to a method for quickly cultivating Baishanzu fir seedlings through embryo rescue technology, so as to achieve the purpose of expanding the number of endangered species. Background technique [0002] Abiesbeshanzuensis is a plant of the genus Abies in the family Pinaceae, an evergreen tree with a height of 17 meters and a diameter at breast height of 80 centimeters. The autumn fruit is cylindrical, with a short stem, 7-12 cm long, 3.5-4 cm in diameter, light brown or light brown yellow when mature. It blooms in May, and the fruit matures in November. It is a unique ancient relict plant in Zhejiang Province. It was widely distributed in Jiangsu, Zhejiang, Anhui, Fujian and other provinces during the Tertiary Period, and it is the only rare species that has survived in some areas after the Quaternary Ice Age. Baishanzu fir is a first-class national key protected plant, and was listed as one of the 12 most endangered plants in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 陈利萍刘柯陈德良吴友贵向珣于明坚曹丽雯于宁宁许大明周荣飞
Owner ZHEJIANG UNIV
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