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A method for rapid cultivation of Baishanzu fir seedlings through embryo rescue technology

A technology of embryo rescue and technology, which is applied in the field of rapid cultivation of Baishanzu fir seedlings, can solve the problems of ecological environment impact, weak sustainable reproduction ability, and difficulty in natural regeneration, and achieve the effect of strong operability and low pollution rate

Active Publication Date: 2020-08-14
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, Baishanzu fir is affected by the ecological environment, which causes problems such as difficulty in natural regeneration and extremely weak sustainable reproduction ability.

Method used

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  • A method for rapid cultivation of Baishanzu fir seedlings through embryo rescue technology
  • A method for rapid cultivation of Baishanzu fir seedlings through embryo rescue technology
  • A method for rapid cultivation of Baishanzu fir seedlings through embryo rescue technology

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Embodiment 1

[0024] Materials: In this example, immature embryos and endosperms of Abies baishanzu were used as explants for culture.

[0025] Step (1): Collect materials

[0026] From late July to early August, the immature cones of Baishanzu fir were collected in Baishanzu Nature Reserve. The color is green. The surface scales and bract scales of this green immature fruit are closely arranged. It can be placed in a sealed bag and stored at a low temperature of 4°C for later use.

[0027] Step (2): Seed Surface Sterilization

[0028] Separate the seed scales and seeds with tweezers, wash the seeds under running water for 3 hours, treat them with 70% ethanol for 30 seconds on an ultra-clean workbench, and rinse them with sterile water for 3 to 5 times. After treating with 0.1wt% mercuric chloride for 10 minutes, rinse with sterile water for 3 to 5 times and dry the surface moisture with sterile filter paper.

[0029] Step (3): Immature embryo isolation and inoculation

[0030] Place th...

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Abstract

The invention discloses a method for rapidly cultivating Baishanzu fir seedlings through embryo rescue technology, aiming to solve the extremely endangered problem of Baishanzu fir. The invention uses the immature cones of the wild plant of Abies baishanzu in the Baishanzu Nature Reserve as the starting material, strips off the immature embryos and endosperm after surface disinfection, and quickly cultivates the wild plants of Abies baishanzu through embryo rescue technology The offspring will obtain a large number of Baishanzu fir seedlings in a short period of time. The advantages of the present invention are: using the immature embryos of Abies baishanzu and cultivating them for 30 days, more seedlings of abies baishanzu can be obtained, the speed of cultivation of abies baishanzu seedlings can be accelerated, and the status quo of abies abies baishanzu being extremely endangered under natural conditions can be improved . The method is highly operable, simple and efficient, and has a low pollution rate, which is of great significance to the rapid cultivation of endangered plant seedlings in the world.

Description

technical field [0001] The invention relates to a method for quickly cultivating Baishanzu fir seedlings through embryo rescue technology, so as to achieve the purpose of expanding the number of endangered species. Background technique [0002] Abiesbeshanzuensis is a plant of the genus Abies in the family Pinaceae, an evergreen tree with a height of 17 meters and a diameter at breast height of 80 centimeters. Cones cylindrical, with short stems, 7-12 cm long, 3.5-4 cm in diameter, light brown or light brown yellow when mature. It blooms in May, and the fruit matures in November. It is a unique ancient relict plant in Zhejiang Province. It was widely distributed in Jiangsu, Zhejiang, Anhui, Fujian and other provinces during the Tertiary Period, and it is the only rare species that has survived in some areas after the Quaternary Ice Age. Baishanzu fir is a first-class national key protected plant, and was listed as one of the 12 most endangered plants in the world by the S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 陈利萍刘柯陈德良吴友贵向珣于明坚曹丽雯于宁宁许大明周荣飞
Owner ZHEJIANG UNIV
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