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Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization

A technology of distant hybridization and radish, applied in horticultural methods, botanical equipment and methods, plant gene improvement, etc., can solve the problem of no systematic research on embryo rescue, and achieve the effect of improving affinity

Inactive Publication Date: 2013-05-29
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For the distant hybridization of radish and turnip, especially when embryo abortion occurs during the hybrid embryo development stage, there is no systematic research at home and abroad on the impact of the best time, best location, and culture conditions for embryo rescue on embryo rescue.

Method used

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  • Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization
  • Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization
  • Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization

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Embodiment Construction

[0024] The present invention is described in detail below:

[0025] 1. Materials and methods

[0026] 1.1 Test materials

[0027] The radish male sterile line DH303A was used as the female parent, and the turnip was used as the male parent. The parental parents were provided by the Vegetable Science and Technology Center of Hubei Academy of Agricultural Sciences.

[0028] 1.2 Test method

[0029] This experiment was carried out at the Vegetable Science and Technology Center of the Economic Crops Research Institute of the Hubei Academy of Agricultural Sciences.

[0030] 1.2.1 Embryo rescue after pod setting

[0031] Pick the pods on the 5th, 6th, and 7th day after pollination, disinfect with 70% ethanol for 0.5 min, then soak in 0.1% HgCl for 8-10 min, rinse with sterile water 3 times, and soak in sterile water for 3 min each time. Before inoculation, dry the sterile water on the surface of the pods with sterile filter paper, peel them off carefully to obtain hybrid embryos...

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Abstract

The invention relates to a method for achieving isolated embryos through radish and turnip intergeneric distant hybridization. The method is characterized by sequentially comprising the following steps of: (1) preparing materials; (2) sowing; (3) bagging and hanging plates; (4) performing pollination; and (5) saving embryos. By the method, the radish and turnip intergeneric distant hybridization isolated embryos are obtained successfully; hybrid embryo seedlings are cultivated; and a new breeding resource is obtained.

Description

technical field [0001] The invention belongs to the field of plant tissue culture rapid propagation, and in particular relates to a method for obtaining isolated embryos by distant hybridization between radish and turnip. Background technique [0002] Radish (Raphanus sativus L.) is one of the main vegetable crops cultivated in our country. It is native to my country and is cultivated in various places. There are many varieties, such as white-skinned radish, red-skinned radish, and green-skinned radish. The roots are edible, either fresh or raw, and the seeds contain 42% oil, which can be used to make soap or lubricating oil. Seeds, fresh roots, and leaves can be used as medicine, which can lower Qi and eliminate accumulation. Raw radish contains amylase, which can help digestion. Kohlrabi (Brassica juncea Coss.var.napiformmis Pal et.Bols) is a variety of mustard. It is mustard for roots. It is a biennial herb of the family Brassica and Brassica. Its product organs are fl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H4/00
Inventor 梅时勇甘彩霞崔磊何云启黄来春王晴芳丁自立张兴中梅雪飞高翔
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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