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Method for obtaining chrysanthemum distance hydrid using goung embryo rescue

A technology of immature embryo rescue and distant hybridization, which is applied in the fields of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of difficult gene introduction and cultivation of chrysanthemums, and achieve the effect of broadening the genetic basis

Inactive Publication Date: 2005-01-12
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Therefore, it is difficult to introduce the good genes in these species into cultivated chrysanthemums [generally hexaploid (2n=6x=54) and its aneuploid] by means of conventional hybridization.

Method used

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  • Method for obtaining chrysanthemum distance hydrid using goung embryo rescue
  • Method for obtaining chrysanthemum distance hydrid using goung embryo rescue
  • Method for obtaining chrysanthemum distance hydrid using goung embryo rescue

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Embodiment Construction

[0031] The method for the rescue of immature embryos of distantly related hybrids of the genus Chrysanthemum and the identification of hybrids provided by the present invention is implemented as follows:

[0032] 1. Obtaining the progeny material of Chrysanthemum interspecific hybridization

[0033] 1.1 Material selection

[0034] Select the diploid wild species Chrysanthemum (D. ". Nanjing: Jiangsu Science and Technology Press, 1993, 4).

[0035] 1.2 Rescue of immature embryos and hybridization

[0036]Hybridization was carried out in the greenhouse. The diploid wild chrysanthemum brain was used as the female parent to cross with the cultivated chrysanthemum 'Huangying'. The female parent was artificially detasseled and bagged for isolation. The male parent was bagged when the tongue-like flowers were slightly open. Pollination is performed at 9-10 o'clock or 3-4 o'clock in the afternoon, and the same inflorescence is pollinated twice. After hybridization, the method of o...

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Abstract

A method for preparing the distant hybrid of chrysanthemum by embryo rescue includes hybridization between wild diplontic chrysanthemum and cultured hexaplontic 'Huang Ying', embryo rescue by ovary culture, discriminating their descendants by different approaches, and choosing the descendant with new characters.

Description

1. Technical field [0001] The invention relates to a method for obtaining distant hybrids of the genus Chrysanthemum by rescuing immature embryos, which relates to a method for chrysanthemum germplasm innovation and breeding of wild species of chrysanthemum native to China and cultivated chrysanthemum varieties, and belongs to the field of biotechnology breeding. 2. Technical Background [0002] my country is the origin center of cultivated chrysanthemum and the distribution center of Chrysanthemum germplasm resources. There are 22 species of 37 kinds of narrow sense Chrysanthemum (Dendranthema) plants distributed in my country, accounting for about 60%. Contains excellent characters that many cultivated chrysanthemums lack, such as the fragrance of Shennongxiangju (D.lavandulifolum var.aromaticum), the drought resistance of Mengju, the resistance of Xiaoshanju, Arctic chrysanthemum (D.arctium), and Alpinum (Chrysanthemum alpinum). Cold. [0003] Chrysanthemum (Dendranthema×g...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 陈发棣李辛雷房伟民崔娜欣管志勇
Owner NANJING AGRICULTURAL UNIVERSITY
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