Method for creating yellow-seeded brassica napus germplasm

A technology of Brassica napus, yellow seed, applied in the field of invention of the creation method

Inactive Publication Date: 2012-01-04
JIANGSU ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0006]Technical Problem The purpose of the present invention is to aim at the current situation that the efficiency of the establishment of yellow seed rape germplasm in my country is not high, and to use the existing yellow seed resources of the original parent of Brassica to create The new germplasm of yellow-seed rapeseed expands the germplasm resources of yellow-seed rapeseed, so as to obtain new genetic resources that can be used commercially, so as to improve the quality of my country's commercial rapeseed and enhance its international competitiveness

Method used

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Embodiment approach

[0036] Implementation plan: In the autumn of 2005, the yellow-seeded cabbage variety Yangyou No. 3 (a variety approved by Jiangsu Province) and the yellow-seeded cabbage variety Qingfeng (a national cabbage approved variety) were planted in the field. In the spring of 2006, Yangyou No. 3 was used as the female parent. In the afternoon of the same day, the cabbage pollen was artificially inoculated, and then bagged to prevent the introduction of foreign pollen. After about 20-25 days, the siliques with enlarged shape (siliques still showing green) were selected for embryo rescue treatment, that is, the embryos were taken out and placed in MS medium. After it grew into seedlings, it was transplanted into the field in the autumn of 2006. In the spring flowering period of 2007, spray 0.5 mg / L colchicine solution on the flower buds of the hybrid plants for 7 days, and find that the anthers have pollen, confirming that the doubling is successful, then carry out artificial self-pollin...

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Abstract

The invention belongs to the field of creation of new crop germplasm, and relates to a distant hybridization technology and a conventional field hybridization technology in brassica of brassicaceae, in particular to a method for creating a new yellow-seeded brassica napus germplasm. The method comprises the following steps: selecting a Chinese cabbage (genome is AA, and n is equal to 10) with yellow seed property and a wild cabbage (genome is CC, and n is equal to 9) with yellow seed property as parents; hybridizing the Chinese cabbage with the wild cabbage, and obtaining allotetraploid hybrid seedlings, namely the brassica napus (genome is AACC, and n is equal to 19) by utilizing an embryo rescue technology and a chromosome doubling technology; harvesting seeds after open pollination during a blooming period; and planting the allotetraploid hybrid seedlings in a field in the next year, hybridizing by manually bagging every two single plants during the blooming period and then harvesting seeds, screening plants with yellow seeds and finally obtaining the yellow-seeded brassica napus germplasm by adopting a pedigree selection method. The method provided by the invention has the advantages of high efficiency and short breeding period, and the obtained yellow-seeded germplasm can be used for breeding a yellow-seeded brassica napus variety.

Description

technical field [0001] The invention belongs to the field of creation of new germplasm of crops, in particular to the invention of a method for creating new germplasm of Brassica napus yellow seeds. It involves distant hybridization technology and conventional field hybridization technology in Brassicaceae Brassica. Background technique [0002] Rapeseed is a cultivated plant of the Brassica genus, and Brassica napus is the main cultivated species of Brassica as an oil crop. Japanese scholars Sheng Yong, Yu Changchun and others (Practical Rapeseed Cultivation, edited by Liu Houli, Shanghai Science and Technology Press, 1987, p37-40), through the study of different interspecific hybridization and hybrid cytology of Brassica plants, proposed The model of "Yu's Triangle" was established, that is, the three basic species of Brassica in Brassicaceae were Brassica cabbage (chromosomal set is CC, n=9), Chinese cabbage (chromosomal set is AA, n=10) and black mustard (chromosomal se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/08C12Q1/68C12Q1/04
Inventor 龙卫华浦惠明高建芹胡茂龙戚存扣张洁夫陈松陈峰顾慧付三雄
Owner JIANGSU ACAD OF AGRI SCI
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