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Sweet potato distant hybridization breeding method with high success rate

A technology of distant hybridization and success rate, applied in horticultural methods, botanical equipment and methods, chemicals for biological control, etc. and other problems to achieve the effect of strong operability and high success rate

Inactive Publication Date: 2010-06-02
XUZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these interspecific hybrids are not real hybrids with two complete sets of chromosomes, but interspecific hybrid materials with a large number of missing chromosomes. These materials have complex chromosome numbers, low fertility, and poor tuberability, which often limit Breeding application
[0003] In summary, whether it is the wild species of Group I or Group II of the sweet potato group, whether it is sexual hybridization or somatic cell hybridization, it is currently possible to There are few wild species successfully hybridized with sweet potato, which is far from meeting people's needs for the utilization of sweet potato relative wild resources
The reasons may be as follows: (1) most of the closely related wild species are located in group II of the sweet potato group, and have poor hybrid compatibility and low fertility with sweet potato; (2) the ploidy of sweet potato and some wild species High, large number, small chromosomes, even if protoplast fusion technology is used to obtain somatic cell hybrids, it often results in the loss of a large number of hybrid chromosomes and hybrid sterility
(3) Sweet potato cultivars lacking broad hybrid compatibility
(4) The flowering period is not met, the workload is heavy, the seed setting rate is low, and the hybrid abortion
(5) Lack of systematic and supporting interspecies hybridization integration technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The specific hybridization method of Xushu 18×I.wrightii successfully hybridized is as follows:

[0015] 1) Parent material preparation: the female parent Xushu 18 seeds were sown in the seedbed at the end of March in spring, and covered with plastic film and a small shed. Wild species I. wrightii (originated in Mexico) and safflower morning glory (rootstock) seed were sown in nutrient pots of 10×10 cm after soaking and accelerating germination. At the beginning of May, Xushu 18 seedlings were cut to make scions, and the safflower morning glory was used as the rootstock for splitting. After the grafts survived, they were moved into hybrid pots for photoperiod treatment. When the wild species has two leaves and one heart (two true leaves are unfolded and one cotyledon is exposed), they are colonized in wild resource gardens.

[0016] 2) Regulation of flowering period: the flowering period of wild species in the open field is from June to September. Grafted Xushu 18 ha...

Embodiment 2

[0023] The specific hybridization method of Xushu 18×I.Lonchophylla successfully crossed is as follows:

[0024] 1) Parent material preparation: the female parent Xushu 18 seeds were sown in the seedbed at the end of March in spring, and covered with plastic film and a small shed. Wild species I. Lonchophylla (originated in Australia) and safflower morning glory (rootstock) seed were sown in a nutrient pot of 10×10 cm after soaking and accelerating germination. At the beginning of May, Xushu 18 seedlings were cut to make scions, and the safflower morning glory was used as the rootstock for splitting. After the grafts survived, they were moved into hybrid pots for photoperiod treatment. When the wild species has two leaves and one heart (two true leaves are unfolded and one cotyledon is exposed), they are colonized in wild resource gardens.

[0025] 2) Regulation of flowering period: the flowering period of the wild species is 6-9 copies in the open field. Grafted Xushu 18 h...

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Abstract

The invention discloses a sweet potato distant hybridization breeding method with a high success rate, and relates to the technical field of plant innovation and new variety breeding of crops. The method comprises the following steps of: taking a wide-compatibility parental sweet potato Xushu 18 as a female parent, and regulating the flower season of the Xushu 18 to be synchronous with that of a wild specie sweet potato by a grafting and dark treatment method; performing water culture on a male parent and the female parent indoor to naturally take root and blossom; after the male parent and the female parent blossom, performing hybrid pollination; after pollinating, coating a hybridization treating fluid (40mg / L 6-benzyladenine, 45mg / L 2,4-dichlorphenoxyacetic acid and 40mg / L naphthylacetic acid) on the stem base of an ovary and the whole flower stalk, and continuously treating for 7 days; and for a combined hybridized ovule which stops early development, adopting immature embryo rescue technology. The hybridization method and a hybridization system of the invention have the advantages of high operability and high success rate. The method is also suitable for indoor directed hybridization of a field naturally blossoming sweet potato material and a conventional sexual hybridization breeding of the sweet potato.

Description

technical field [0001] The invention relates to crop germplasm innovation and new variety breeding technology, and relates to the sexual hybridization technology of sweet potato and its relative wild species. Specifically, it is a sweet potato distant hybrid breeding method with high success rate. Background technique [0002] The wild resources of sweet potato relatives are quite rich, and there are 600-700 species of sweet potato distributed in the world. Among them, many wild species have excellent genes such as resistance to diseases and insect pests, high dry rate, and stress resistance. Jiangsu Academy of Agricultural Sciences reported that most wild species of the genus Ipomoea introduced in 1977 had stem nematode resistance genes and high-yield potential genes; Shandong Academy of Agricultural Sciences reported in 1984 that I. The offspring of the cross can appear the super-parent variation of dry rate. However, due to the severe interspecific hybrid incompatibili...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00A01N43/90A01N39/04A01N37/10A01P21/00
Inventor 曹清河马代夫李强李秀英王欣后猛
Owner XUZHOU ACAD OF AGRI SCI
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