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Medicinal wild rice embryo rescue rapid progeny propagation culture medium and method

A technology of medicinal wild rice and rooting medium, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of low seedling rate, poor stress resistance of tissue culture seedlings, etc., and achieve fast proliferation, genetic The effect of consistency and short production cycle

Active Publication Date: 2017-01-11
NINGBO ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problems of poor stress resistance and low seedling growth rate of tissue cultured plantlets in the process of rapid propagation of medicinal wild rice embryo rescued offspring caused by the composition of the medium in the above-mentioned prior art, the present invention provides an improved medicinal wild rice Culture medium and method for rapid propagation of rice embryo rescued offspring to achieve the purpose of healthy and rapid growth of tissue culture seedlings in the process of rapid propagation of medicinal wild rice embryo rescued offspring

Method used

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  • Medicinal wild rice embryo rescue rapid progeny propagation culture medium and method

Examples

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Effect test

Embodiment 1

[0041] 1) Pretreatment of medicinal wild rice embryo-rescued offspring seeds: put medicinal wild rice embryo-rescued offspring seeds into sterilized fine sand and heat-treat them at 37±2°C for 6-12 weeks to seedlings; After sterilizing with 70% alcohol for 30 seconds, adding one drop of 0.1% mercuric chloride, shaking for 8 minutes and washing with sterile water for 3-5 times, it is used as an explant for later use;

[0042] 2) Induction culture: inoculate the seeds on the induction medium, the formula of the induction medium is MS+2.4D2.0mg / l+6BA0.3mg / l; the culture conditions are at 25±2°C, light intensity 1000lux, Light for 16 hours / day, increase to 2000lux after four weeks, increase to 4000lux after six weeks, culture for 1.5-2 months until embryogenic cells are induced;

[0043]3) Differentiation culture: Inoculate embryogenic cells on the differentiation medium, the formula of the differentiation medium is MS+IAA 1.8mg / l+6BA 2.5mg / l+KT 1.2mg / l; the culture condition is a...

Embodiment 2

[0046] 1) Pretreatment of medicinal wild rice embryo rescued offspring seeds: put the medicinal wild rice embryo rescued offspring seeds into sterilized fine sand and heat-treat them at 37±2°C for 6-12 weeks until the seedlings; take 1-2cm of young shoots, After being sterilized with 70% alcohol for 30 seconds, adding one drop of 0.1% mercuric chloride, shaking for 8 minutes and washing with sterile water for 3-5 times, it was used as an explant for later use;

[0047] 2) Induction culture: inoculate the seeds on the induction medium, the formula of the induction medium is S+2.4D 1.0mg / l+6BA 0.1mg / l; the culture conditions are at 25±2°C, light intensity 1000lux, Light for 16 hours / day, increase to 2000lux after four weeks, and increase to 4000lux after six weeks, culture for 1.5-2 months until embryogenic cells are induced;

[0048] 3) Differentiation culture: Inoculate embryogenic cells on the differentiation medium, the formula of the differentiation medium is MS+IAA 1.0mg / l...

Embodiment 3

[0051] 1) Pretreatment of medicinal wild rice embryo rescued offspring seeds: put the medicinal wild rice embryo rescued offspring seeds into sterilized fine sand and heat-treat them at 37±2°C for 6-12 weeks until the seedlings; take 1-2cm of young shoots, After being sterilized with 70% alcohol for 30 seconds, adding one drop of 0.1% mercuric chloride, shaking for 8 minutes and washing with sterile water for 3-5 times, it was used as an explant for later use;

[0052] 2) Induction culture: inoculate the seeds on the induction medium, the formula of the induction medium is MS+2.4D3.0mg / l+6BA0.5mg / l; the culture conditions are at 25±2°C, light intensity 1000lux, Light for 16 hours / day, increase to 2000lux after four weeks, and increase to 4000lux after six weeks, culture for 1.5-2 months until embryogenic cells are induced;

[0053] 3) Differentiation culture: Inoculate embryogenic cells on the differentiation medium, the formula of the differentiation medium is MS+IAA3.0mg / l+6...

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Abstract

The invention provides a medicinal wild rice embryo rescue rapid progeny propagation culture medium. The medicinal wild rice embryo rescue rapid progeny propagation culture medium comprises an induction culture medium, a differentiation culture medium and a rooting culture medium, wherein the formula of the induction culture medium is MS+2.4D1.0-3.0mg / l+6BA0.1-0.5mg / l; the formula of the differentiation culture medium is MS+IAA1.0-3.0mg / l+6BA2.0-4.0mg / l+KT1.0-2.0mg / l; and the formula of the rooting culture medium is 1 / 2MS+IAA0.02-0.5mg / l. The invention also provides a medicinal wild rice embryo rescue rapid progeny propagation method. According to the medicinal wild rice embryo rescue rapid progeny propagation culture medium and method provided by the invention, healthy and rapid growth of tissue culture seedlings can be realized a short period of time.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation, in particular to a culture medium and method for rapid propagation of medicinal wild rice embryo rescued offspring. Background technique [0002] Wild rice is a valuable germplasm for rice breeding. Breeding studies at home and abroad have shown that wild rice with AA genome has played a huge role in rice breeding. Breeders recognize that to obtain breakthrough breeds, new parentages must be found. It can be seen that it is imperative to use wild rice as parents. But because cultivated rice is the rice seed with AA type chromosome set and common wild rice hybridization is easy to succeed, and the rice seed hybridization with non-AA type chromosome set, often leads to failure with general conventional sexual hybridization method. In wild rice, the wild rice with non-AA chromosome group often has excellent characters that are rare in cultivated and common wild rice...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 严成其陈剑平王芳鲍根良杨勇余初浪王栩鸣周洁黄坚沈岚朱宏芬张国芳刘健程晔颜秋生
Owner NINGBO ACAD OF AGRI SCI
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