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Culture medium and method for rapid propagation of somatic hybrid offspring of Oryza verruca

A technology for inducing culture medium and differentiation medium is applied in the field of culture medium for rapid propagation of somatic cell hybridization offspring of wild rice verruciformis. The effect of fast proliferation and short production cycle

Active Publication Date: 2019-02-19
NINGBO ACAD OF AGRI SCI +1
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] In order to solve the technical problems of poor stress resistance and low seedling rate of tissue cultured plantlets in the rapid propagation process of the somatic hybrid offspring of wild rice verruca due to the composition of the medium in the above-mentioned prior art, the present invention provides an improved verruca Medium and method for rapid propagation of wild rice somatic hybrid progeny, in order to achieve healthy growth and high seedling rate of tissue cultured seedlings in the process of rapid propagation of wild rice somatic hybrid progeny

Method used

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  • Culture medium and method for rapid propagation of somatic hybrid offspring of Oryza verruca

Examples

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Effect test

Embodiment 1

[0041]1) Pretreatment of seeds of somatic hybrid progeny of O. verruca: Disinfect the seeds of somatic hybrid progeny of O. verruca with 70% alcohol for 30 seconds, add one drop of 0.1% mercury chloride, shake for 8 minutes and wash with sterile water for 3-5 After the second time, it was used as an explant for later use;

[0042] 2) Induction culture: inoculate the seeds on the induction medium, the formula of the induction medium is MS+2,4-D 1.8mg / l+6-BA 0.2mg / l; the culture condition is at 25±2°C , light intensity 1000lux, light 16 hours / day, increase to 2000lux after four weeks, increase to 4000lux after six weeks, culture for 1.5-2 months until embryogenic cells are induced;

[0043] 3) Differentiation culture: inoculate embryogenic cells on the differentiation medium, the formula of the differentiation medium is MS+NAA1.8mg / l+6-BA0.2mg / l+KT 1mg / l; the culture condition is at 25 ±2°C, light intensity 2000-3000lux, light 16 hours / day, 1 month to grow into 8-10cm long seed...

Embodiment 2

[0046] 1) Pretreatment of seeds of somatic hybrid progeny of O. verruca: Disinfect the seeds of somatic hybrid progeny of O. verruca with 70% alcohol for 30 seconds, add one drop of 0.1% mercury chloride, shake for 8 minutes and wash with sterile water for 3-5 After the second time, it was used as an explant for later use;

[0047] 2) Induction culture: inoculate the seeds on the induction medium, the formula of the induction medium is MS+2,4-D 1.5mg / l+6-BA 0.1mg / l; the culture condition is at 25±2°C , light intensity 1000lux, light 16 hours / day, increase to 2000lux after four weeks, increase to 4000lux after six weeks, culture for 1.5-2 months until embryogenic cells are induced;

[0048] 3) Differentiation culture: inoculate embryogenic cells on the differentiation medium, the formula of the differentiation medium is MS+NAA1.5mg / l+6-BA0.1mg / l+KT0.5mg / l; the culture condition is at 25±2℃, light intensity 2000-3000lux, light 16 hours / day, 1 month to grow into 8-10cm long seed...

Embodiment 3

[0051] 1) Pretreatment of seeds of somatic hybrid progeny of O. verruca: Disinfect the seeds of somatic hybrid progeny of O. verruca with 70% alcohol for 30 seconds, add one drop of 0.1% mercury chloride, shake for 8 minutes and wash with sterile water for 3-5 After the second time, it was used as an explant for later use;

[0052] 2) Induction culture: inoculate the seeds on the induction medium, the formula of the induction medium is MS+2,4-D 2.2mg / l+6-BA 0.3mg / l; the culture condition is at 25±2°C , light intensity 1000lux, light 16 hours / day, increase to 2000lux after four weeks, increase to 4000lux after six weeks, culture for 1.5-2 months until embryogenic cells are induced;

[0053] 3) Differentiation culture: Inoculate embryogenic cells on the differentiation medium, the formula of the differentiation medium is MS+NAA2.5mg / l+6-BA 0.3mg / l+KT 3mg / l; the culture condition is at 25 ±2°C, light intensity 2000-3000lux, light 16 hours / day, 1 month to grow into 8-10cm long se...

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Abstract

The invention provides a medium for rapid breeding of Oryza granulate somatic hybridization generation. The medium comprises an induction medium, a differentiation medium and a rooting medium. The induction medium comprises 1.5-2.2mg / L of MS+2.4D and 0.1-0.3mg / L of 6BA. The differentiation medium comprises 1.5-2.5mg / L of MS+NAA, 0.1-0.3mg / L of 6BA and 0.5-3mg / L of KT. The rooting medium comprises 0.01-0.3mg / L of 1 / 2MS+NAA. The invention also provides a method for rapid breeding of Oryza granulate somatic hybridization generation. The medium and method realize high efficiency, healthy and fast growth of a tissue culture seedling.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture and rapid propagation, in particular to a culture medium and a method for rapid propagation of somatic cell hybrid offspring of O. verruca. Background technique [0002] Wild rice is a valuable germplasm for rice breeding. Breeding studies at home and abroad have shown that wild rice with AA genome has played a huge role in rice breeding. Breeders recognize that to obtain breakthrough breeds, new parentages must be found. It can be seen that it is imperative to use wild rice as parents. But because cultivated rice is the rice seed with AA type chromosome set and common wild rice hybridization is easy to succeed, and the rice seed hybridization with non-AA type chromosome set, often leads to failure with general conventional sexual hybridization method. In wild rice, the wild rice with non-AA chromosome group often has excellent characters that are rare in cultivated and common wild...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 严成其陈剑平王芳鲍根良杨勇余初浪王栩鸣周洁黄坚沈岚朱宏芬张国芳刘健程晔颜秋生
Owner NINGBO ACAD OF AGRI SCI
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