Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Medium and method for rapid propagation of offspring rescued by medicinal wild rice embryos

A technology for medicinal wild rice and rooting medium, which is applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of poor stress resistance and low seedling rate of tissue culture seedlings, and achieves consistent heredity and proliferation. The effect of fast speed and short production cycle

Active Publication Date: 2018-06-05
NINGBO ACAD OF AGRI SCI +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problems of poor stress resistance and low seedling growth rate of tissue cultured plantlets in the process of rapid propagation of medicinal wild rice embryo rescued offspring caused by the composition of the medium in the above-mentioned prior art, the present invention provides an improved medicinal wild rice Culture medium and method for rapid propagation of rice embryo rescued offspring to achieve the purpose of healthy and rapid growth of tissue culture seedlings in the process of rapid propagation of medicinal wild rice embryo rescued offspring

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medium and method for rapid propagation of offspring rescued by medicinal wild rice embryos

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1) Pretreatment of medicinal wild rice embryo rescued offspring seeds: put the medicinal wild rice embryo rescued offspring seeds into sterilized fine sand and heat-treat them at 37±2°C for 6-12 weeks until the seedlings; take 1-2cm of young shoots, After being sterilized with 70% alcohol for 30 seconds, adding one drop of 0.1% mercuric chloride, shaking for 8 minutes and washing with sterile water for 3-5 times, it was used as an explant for later use;

[0042] 2) Induction culture: inoculate the seeds on the induction medium, the formula of the induction medium is MS+2.4D2.0mg / l+6BA0.3mg / l; the culture conditions are at 25±2°C, light intensity 1000lux, Light for 16 hours / day, increase to 2000lux after four weeks, and increase to 4000lux after six weeks, culture for 1.5-2 months until embryogenic cells are induced;

[0043]3) Differentiation culture: Inoculate embryogenic cells on the differentiation medium, the formula of the differentiation medium is MS+IAA 1.8mg / l+6...

Embodiment 2

[0046] 1) Pretreatment of medicinal wild rice embryo rescued offspring seeds: put the medicinal wild rice embryo rescued offspring seeds into sterilized fine sand and heat-treat them at 37±2°C for 6-12 weeks until the seedlings; take 1-2cm of young shoots, After being sterilized with 70% alcohol for 30 seconds, adding one drop of 0.1% mercuric chloride, shaking for 8 minutes and washing with sterile water for 3-5 times, it was used as an explant for later use;

[0047] 2) Induction culture: inoculate the seeds on the induction medium, the formula of the induction medium is S+2.4D 1.0mg / l+6BA 0.1mg / l; the culture conditions are at 25±2°C, light intensity 1000lux, Light for 16 hours / day, increase to 2000lux after four weeks, and increase to 4000lux after six weeks, culture for 1.5-2 months until embryogenic cells are induced;

[0048] 3) Differentiation culture: Inoculate embryogenic cells on the differentiation medium, the formula of the differentiation medium is MS+IAA 1.0mg / l...

Embodiment 3

[0051] 1) Pretreatment of medicinal wild rice embryo rescued offspring seeds: put the medicinal wild rice embryo rescued offspring seeds into sterilized fine sand and heat-treat them at 37±2°C for 6-12 weeks until the seedlings; take 1-2cm of young shoots, After being sterilized with 70% alcohol for 30 seconds, adding one drop of 0.1% mercuric chloride, shaking for 8 minutes and washing with sterile water for 3-5 times, it was used as an explant for later use;

[0052] 2) Induction culture: inoculate the seeds on the induction medium, the formula of the induction medium is MS+2.4D3.0mg / l+6BA0.5mg / l; the culture conditions are at 25±2°C, light intensity 1000lux, Light for 16 hours / day, increase to 2000lux after four weeks, and increase to 4000lux after six weeks, culture for 1.5-2 months until embryogenic cells are induced;

[0053] 3) Differentiation culture: Inoculate embryogenic cells on the differentiation medium, the formula of the differentiation medium is MS+IAA3.0mg / l+6...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a medicinal wild rice embryo rescue rapid progeny propagation culture medium. The medicinal wild rice embryo rescue rapid progeny propagation culture medium comprises an induction culture medium, a differentiation culture medium and a rooting culture medium, wherein the formula of the induction culture medium is MS+2.4D1.0-3.0mg / l+6BA0.1-0.5mg / l; the formula of the differentiation culture medium is MS+IAA1.0-3.0mg / l+6BA2.0-4.0mg / l+KT1.0-2.0mg / l; and the formula of the rooting culture medium is 1 / 2MS+IAA0.02-0.5mg / l. The invention also provides a medicinal wild rice embryo rescue rapid progeny propagation method. According to the medicinal wild rice embryo rescue rapid progeny propagation culture medium and method provided by the invention, healthy and rapid growth of tissue culture seedlings can be realized a short period of time.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation, in particular to a culture medium and method for rapid propagation of medicinal wild rice embryo rescued offspring. Background technique [0002] Wild rice is a valuable germplasm for rice breeding. Breeding studies at home and abroad have shown that wild rice with AA genome has played a huge role in rice breeding. Breeders recognize that to obtain breakthrough breeds, new parentages must be found. It can be seen that it is imperative to use wild rice as parents. But because cultivated rice is the rice seed with AA type chromosome set and common wild rice hybridization is easy to succeed, and the rice seed hybridization with non-AA type chromosome set, often leads to failure with general conventional sexual hybridization method. In wild rice, the wild rice with non-AA chromosome group often has excellent characters that are rare in cultivated and common wild rice...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 严成其陈剑平王芳鲍根良杨勇余初浪王栩鸣周洁黄坚沈岚朱宏芬张国芳刘健程晔颜秋生
Owner NINGBO ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com