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Method for performing isolated culture on radish and kohlrabi intergeneric hybrid embryos

A technology of in vitro culture and hybrid embryos, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve problems such as different flavors, and achieve the effect of facilitating innovation

Inactive Publication Date: 2011-10-12
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both radish and kohlrabi are root vegetables, but their flavors are different. So far, there has been no report on the study of distant hybridization between the two

Method used

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  • Method for performing isolated culture on radish and kohlrabi intergeneric hybrid embryos
  • Method for performing isolated culture on radish and kohlrabi intergeneric hybrid embryos
  • Method for performing isolated culture on radish and kohlrabi intergeneric hybrid embryos

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Experimental program
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Effect test

Embodiment

[0026] 1 Materials and methods

[0027] 1.1 Materials

[0028] Seven plants of radish DH303A male sterile line (2n=18) were used as female parents, which were listed as A, B, C, D, E, F, G plants respectively, and kohlrabi was used as male parents. The experiment was completed at the vegetable science and technology research center base of Hubei Academy of Agricultural Sciences.

[0029] 1.2 Method

[0030] 1.2.1 Hybridization method

[0031] After the female parent bolts and before flowering, isolate a single plant to prevent pollination with other cruciferous plants; after flowering, take the pollinated flowers of the male parent to repeatedly pollinate the female parent, and mark the hybrid combination, number of flowers and pollination time. After pollination, the growth and development of the pods were followed and observed, and the length and transverse diameter of the hybrid pods were measured.

[0032] 1.2.2 Rescue of hybrid embryos

[0033] Because in distant hyb...

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PUM

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Abstract

The invention relates to a method for performing isolated culture on radish and kohlrabi intergeneric hybrid embryos. The method is characterized by sequentially comprising the following steps of: (1) preparing materials; (2) sowing; (3) hybridizing; and (4) saving embryos. By the method, the radish and kohlrabi intergeneric hybrid embryos are obtained successfully; hybrid embryo seedlings are cultivated; and a new breeding resource is obtained.

Description

technical field [0001] The invention relates to the field of plant tissue culture rapid propagation, in particular to a method for in vitro culture of hybrid embryos between radish and kohlrabi. Background technique [0002] Radish (Raphanus sativus L.) is one of the main vegetable crops cultivated in our country. It is native to my country and is cultivated in various places. There are many varieties, such as white-skinned radish, red-skinned radish, and green-skinned radish. The roots are edible, either fresh or raw, and the seeds contain 42% oil, which can be used to make soap or lubricating oil. Seeds, fresh roots, and leaves can be used as medicine, which can lower Qi and eliminate accumulation. Raw radish contains amylase, which can help digestion. Kohlrabi (Brassica oleracea var. carlorapa D.C.) is also known as kohlrabi, and its common names are Bolan and Yumanqing. Brassicaceae, Brassica, one or two annual herbaceous plants. Its edible part is a fleshy corm, whi...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
Inventor 梅时勇甘彩霞崔磊何云启黄来春王晴芳丁自立张兴中梅雪飞高翔
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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