Novel method for obtaining artificially synthesized hexaploid wheat

An artificial synthesis and hexaploid technology, applied in the field of crop genetics and breeding, can solve the problems of high manpower, material resources, low survival rate, cumbersome steps, etc., and achieve strong hybrid affinity, expand genetic background, and enrich genetic diversity. Effect

Inactive Publication Date: 2013-03-20
HENAN UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, the limitation of this technology is that every time a tetraploid wheat-Jiejie wheat artificial synthetic species is obtained, the immature embryo rescue technology must be used to obtain the distant hybrid F1 and the colchicine artificial doubling method mus

Method used

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  • Novel method for obtaining artificially synthesized hexaploid wheat

Examples

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Effect test

Embodiment 1

[0020] A new method for obtaining artificially synthesized hexaploid wheat, comprising the following steps:

[0021] 1) Distant hybridization: in mid-to-late April, the female parent was the wheat japonica (collection place: Xinxiang, Henan, provided by the Plant Germplasm Resources and Genetic Engineering Laboratory of Henan University, No. T015) containing the gene for panicle germination resistance, and the common Wheat Zhoumai 16 was used as the male parent to prepare a hybrid combination, and 50 florets of better-growing spikes were selected and artificially detasselled. After emasculation, they were bagged and isolated. When the pistil is mature, the pollen of the mature wheat Zhoumai 16 is applied to the stigma of the pistil of Aesthenia japonicus for artificial pollination. After 14 days of pollination, the hybrid embryos are taken to rescue the young embryos, and the embryos are induced to germinate to obtain test-tube plantlets; Move them to flower pots in November. ...

Embodiment 2

[0024] Wild emmer KU-14493 was used to replace wild emmer KU-13441, and other references were made to Example 1 to obtain artificial hexaploid wheat S-T015-Zhoumai 16-KU-14493 derived from wild emmer.

Embodiment 3

[0026] Wild emmer KU-11557 was used to replace wild emmer KU-13441, and other references were made to Example 1 to obtain artificial hexaploid wheat S-T015-Zhoumai 16-KU-11557 derived from wild emmer.

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Abstract

The invention relates to a novel method for obtaining artificially synthesized hexaploid wheat. The novel method comprises the steps of distant hybridization and obtaining the artificially synthesized hexaploid wheat. The distant hybridization includes that the distant hybridization is carried out with aegilops tauschii as a female parent and common wheat as a male parent, young embryo rescue is carried out through a hybrid embryo, and a test-tube plantlet is obtained; and the test-tube plantlet grows over a summer, manual doubling germination is carried out in November by means of colchicines, normal cultivation management proceeds, and aegilops tauschii-wheat octaploid seeds are obtained the next year. The steps of obtaining the artificially synthesized hexaploid wheat include that hybrid is carried out by means of the obtained aegilops tauschii-wheat octaploid seeds and tetraploid wheat with various good genes, and the hexaploid wheat is directly manually obtained. The novel method is easy to carry out, and the hexaploid wheat germplasm with a rich genetic background can be conveniently and manually synthesized.

Description

technical field [0001] The invention belongs to the technical field of crop genetics and breeding, and specifically relates to a method for synthesizing artificial hexaploid wheat by distant hybridization between wheat and close relatives of wheat, which can more conveniently artificially synthesize hexaploid wheat species with rich genetic background quality. Background technique [0002] wheat( Triticum aestivum L. 2n=6x=42, AABBDD) is one of the main food crops in my country. For a long time, with the efforts of the majority of breeders, a large number of wheat varieties (lines) have been cultivated and promoted. These varieties (lines) have played an important role in the progress of agricultural science and technology and the growth of food in our country. However, the vast majority of these varieties are bred through hybridization between wheat varieties. Although the bred varieties have good comprehensive agronomic traits, due to long-term directional genetic impro...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 张大乐苏亚蕊李玉阁高安礼黄世全李锁平
Owner HENAN UNIVERSITY
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