Molecular marking method of soybean low phosphorus-resistant gene GmAPt

A molecular marker, low-phosphorus-resistant technology, applied in the field of molecular genetics, can solve problems such as low accuracy, achieve the effect of large workload, long cycle, and broaden genetic background
CN101921758BInactive Publication Date: 2012-05-23NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2012-05-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a molecular marking method of a soybean low phosphorus-resistant gene GmAPt, belonging to the field of molecular genetics. A marker Indel_S170 is designed and synthesized by utilizing 10bp deletion existing in the first introns of the soybean low phosphorus-resistant gene GmAPt in a soybean low phosphorus-resistant material and a low phosphorus-nonresistant material, and because the marker is the self marker of the gene, the marker expresses coseparation with the low phosphorus-resistant gene GmAPt and allelic genes thereof. By utilizing the marker, the low-phosphorusresistance of a soybean germ-plasm resource can be accurately and rapidly identified, the selecting efficiency and the split-type identifying efficiency of the soybean low phosphorus-resistant material are greatly improved, and the auxiliary selecting and breeding progress of a soybean low phosphorus-resistant molecular marking is quickened.
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Description

1. Technical field

[0001] The invention provides a molecular marker method of soybean low phosphorus tolerance gene GmAPt, which belongs to the field of molecular genetics and can be used for soybean low phosphorus tolerance molecular marker assisted selection breeding and germplasm typing identification. 2. Background technology

[0002] Soybean is an important food crop and oil crop in my country. However, phosphorus deficiency in two-thirds of the arable land is the main factor limiting soybean production in my country. Therefore, exploring the potential of soybean itself and efficiently utilizing the potential phosphorus resources in the soil is an effective way to solve the phosphorus deficiency of soybean.

[0003] Although some progress has been made in the study of soybean phosphorus efficiency, there are still great difficulties in the screening of phosphorus efficient genotypes, mainly because of the difficulty in determining the screening index and screening peri...

Claims

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