Molecular marking method of soybean low phosphorus-resistant gene GmAPt
A molecular marker, low-phosphorus-resistant technology, applied in the field of molecular genetics, can solve problems such as low accuracy, achieve the effect of large workload, long cycle, and broaden genetic background
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0021] (1) Test materials
[0022] The test materials included 14 materials (Table 1, all provided by the National Soybean Improvement Center) from soybean low-phosphorus-tolerant germplasm reported in different literatures (Liu Ying et al., 2007; Cui Shiyou et al., 2007; Ding Yuchuan et al., 2006) , including 6 low-phosphorus-tolerant germplasms, 7 low-phosphorus-intolerant germplasms and 1 medium-tolerant germplasm.
[0023] Table 1 14 different materials related to low phosphorus resistance
[0024]
[0025] (2) Acquisition of Indel S170 mark
[0026] According to a 10bp deletion site inside the GmAPt gene sequence, a pair of Indel marker primers were designed, the upstream primer was 5'-CTTGAGATCACTACAACACACC-3', and the downstream primer was 5'-GATAGAAGAAC AACACAAAAAC-3'
[0027] (3) Extraction of DNA
[0028] Using fresh leaves at the seedling stage as materials, DNA was extracted by the CTAB method. The detailed steps are as follows:
[0029] 1) Take about 3-5 gr...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com