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A method for rapid and precise breeding of three-line rice restorer lines using rice genomics technology

A genomics and restorer line technology, applied in the fields of botanical equipment and methods, plant gene improvement, and application, can solve the problems of insufficient aggregation of comprehensive excellent traits, long years of breeding three-line restorer lines, etc., to reduce blindness, Shorten the improvement generation and expand the effect of the genetic background

Active Publication Date: 2021-03-02
WIN ALL HI TECH SEED CO LTD +3
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art, and to provide a method for quickly and accurately breeding three-line restorer lines using rice genomics technology, aiming to solve the problems of long years and comprehensive Problems such as insufficient aggregation of excellent properties

Method used

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  • A method for rapid and precise breeding of three-line rice restorer lines using rice genomics technology
  • A method for rapid and precise breeding of three-line rice restorer lines using rice genomics technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] This example provides a rapid and accurate breeding of rice with more excellent traits by using the whole genome technology of rice, using "Huanghuazhan" with better plant type, fertilizer tolerance, lodging resistance and high seed setting rate as the parent to be improved The method of restoring new varieties, its technical roadmap is as follows: figure 1 shown, including the following steps:

[0024] (1) hybridization

[0025] "Huanghuazhan" was used as the female parent, and the restorer line "Huazhan" with excellent agronomic traits, early maturation, rice blast resistance Pi2 gene, and good quality was used as the male parent. The male parent was backcrossed once, and the obtained BC1F1 generation was selfed again to obtain 1200 BC1F2 segregation populations.

[0026] (2) Screening of field agronomic traits

[0027] Screen the BC1F2 generation segregation population in step (1) for field agronomic traits, select 126 excellent individual plants with better plant...

Embodiment 2

[0049] This example provides a rapid and accurate selection of rice with more excellent traits by using the rice genome-wide technology and using "9311" with better plant type, fertilizer tolerance, lodging resistance and high seed setting rate as the parent to be improved The method of restoring new varieties, its technical roadmap is as follows: figure 1 shown, including the following steps:

[0050] (1) hybridization

[0051] "9311" was used as the female parent, and the restorer line "YR0822" with excellent agronomic traits, early maturation, rice blast resistance Pi2 gene, and good quality was used as the male parent. This backcross was once, and the BC1F1 generation obtained was self-crossed again, and 1100 BC1F2 isolated populations were obtained.

[0052] (2) Screening of field agronomic traits

[0053] Screen the BC1F2 segregation population in step (1) for field agronomic traits, select 119 excellent individual plants with better plant and leaf morphology, continu...

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Abstract

The invention discloses a method for selecting and breeding a three-line rice restoring line by a rice genomic technology rapidly and accurately. According to the method, the conventional breeding iscombined with molecular marker auxiliary screening, genetic background screening is conducted by a genotyping technology based on rice whole genome sequencing, and the restoring line which contains ahomozygous sterility restoring gene and has the genetic background closer to a target parent can be screened and obtained. Compared with the prior art, the method has the following advantages: the improved generation of the restoring line is greatly shortened, and the three-line rice restoring line with excellent comprehensive characters can be selected and bred accurately for only 3 to 4 years.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for rapidly and accurately selecting a three-line rice restorer line by using rice genomics technology. Background technique [0002] With the continuous improvement of people's living standards, the demand for high-quality rice with high yield, good appearance and palatability is increasing. It is particularly important to improve the quality of hybrid rice, and breeding high-quality restorer lines with high combining ability is an effective way to improve the quality of hybrid rice. In order to improve the overall level of rice restorer lines and achieve major breakthroughs in high quality, high yield, stable yield, lodging resistance, disease resistance, high combining ability, and wide adaptability of the selected combinations, it is necessary to further improve the restoration line selection. Advantages of force and its optional combination. The current prac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04
CPCA01H1/04
Inventor 张从合韩斌黄学辉王慧陈金节蒋家月冯旗严志方玉王阿红黄艳玲
Owner WIN ALL HI TECH SEED CO LTD
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