11 results about "Genotyping Techniques" patented technology

This application provides a method, apparatus, and electronic device for evaluating the effectiveness of SNP genotyping based on silhouette coefficients, relating to the field of SNP genotyping technology, and alleviating the technical problems in evaluating the effectiveness of SNP genotyping. The method includes: acquiring endpoint fluorescence intensity data for a target SNP locus; performing clustering based on the endpoint fluorescence intensity data using a clustering algorithm to obtain a first clustering result; filtering samples in the first clustering result using individual silhouette coefficients and a preset silhouette coefficient threshold to obtain a sample filtering result; and clustering the sample filtering result using the clustering algorithm to obtain a second clustering result.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to feed intake of Ningxiang pigs and application of the SNP molecular marker. The molecular marker comprises an SNP1 and an SNP2; the SNP1 molecular marker takes a Sus Scrofa Build11.1 genome as a reference genome and is located at the 206264506th site of a No.1 chromosome, the polymorphism of the SNP1 molecular marker is G / A, and the serial number of the SNP1 molecular marker is rs333936191; the SNP2 molecular marker takes a Sus Scrofa Build11.1 genome as a reference genome and is located at the 20668623rd site of a No.1 chromosome, the polymorphism of the SNP2 molecular marker is C / T, and the serial number of the SNP2 molecular marker is rs326767841. According to the invention, a genetic typing technical system suitable for high-throughput KASP detection is constructed, rapid and accurate identification and large-scale screening of the Ningxiang pig FI character can be realized, and the breeding efficiency of individuals with excellent ingestion characters is remarkably improved. And a technical support is provided for genetic resource protection and sustainable utilization of local pig breeds.

The invention belongs to the technical field of genotyping, and particularly relates to a Tan sheep 50K liquid phase chip based on targeted capture sequencing and application thereof, the Tan sheep 50K liquid phase chip is composed of independently packaged probe mixed liquid and a hybridization capture reagent, the independently packaged probe mixed liquid comprises a Tan sheep 50K probe, and site information of the Tan sheep 50K probe relates to 50000 SNP sites. The prepared Tan sheep 50K liquid chip is mixed with a Tan sheep high-throughput sequencing library, a target site is captured and amplified and purified, and after a product is subjected to high-throughput sequencing, a sequencing result is compared with a sheep reference genome, so that the genetic typing of the Tan sheep to be detected is obtained. The method is applicable to resource evaluation, genetic identification and genome selection of the Tan sheep variety, can break through key technical evaluation restricting genome selection breeding, lays a foundation for building a Tan sheep genome selection sharing platform, and accelerates the realization of the Tan sheep from phenotype selection to genome selection.

The present application relates to a method and system for breeding of Marsupenaeus japonicus based on selection of uniformity of growth traits, belonging to the technical field of genetic breeding of Marsupenaeus japonicus. A plurality of breeding base parents of Marsupenaeus japonicus are obtained, a plurality of the breeding base parents are identified and evaluated by genotyping technology, and a plurality of the genomic combination information is introduced into a blank pairing table to obtain a uniformity pairing table of growth traits of Marsupenaeus japonicus. The coefficient of variation of the simulated growth record information is calculated based on the pairing table, the abnormality determination result of the growth traits is generated, the correlation degree of the operating mechanism and the environmental data is calculated based on the abnormality determination result of the growth traits to generate a correlation degree ranking table, the regulation scheme of the breeding equipment is formulated according to the correlation degree ranking table, the influence degree of the seedlings after regulation is calculated by establishing a linear regression model, and the final breeding scheme is obtained. The present application can solve the problem of genetic gene mutation caused by environmental differences in the breeding process of Marsupenaeus japonicus and improve the uniformity of growth traits.

Methods and compositions for nucleic acid amplification, detection, and genotyping techniques are disclosed. In one embodiment, a nucleic acid molecule having a target-specific primer sequence; an anti-tag sequence 5′ of the target-specific primer sequence; a tag sequence 5′ of the anti-tag sequence; and a blocker between the anti-tag sequence and the tag sequence is disclosed. Compositions containing such a nucleic acid molecule and methods of using such a nucleic acid molecule are also disclosed.

The present application relates to the technical field of molecular diagnosis and genotyping, and particularly relates to a VTE high-risk individual identification multiplex isothermal amplification detection method and a VTE high-risk individual identification multiplex isothermal amplification detection kit, a 4-7 SNP site detection panel containing SERPINC1 antithrombin deficiency variation, PROC protein C defect hotspot (p.Arg189Trp) and at least one SNP site in MTHFR, PAI-1, FGG and ABO is constructed; an allele-specific forward primer pair with a 3' end plus the second or third artificial mismatch double-mismatch anchoring structure, a universal reverse primer and a tetrahydrofuran site probe are designed for each SNP site; a risk stratification result is output by using an OR value weighted scoring model of a Chinese population cohort, the detection is rapid, single-tube multiplex, sample consumption is low, zero-instrument optional, and is suitable for rapid screening of genetic risk of venous thromboembolism in primary medical institutions of Chinese population.

The application provides a SNP molecular marker for detecting cucumber fruit top shape, a KASP primer and application thereof. The SNP molecular marker is closely linked to the cucumber fruit top shape, is located at the position of 25221828 of a Chinese Long v3 genome Chr01 chromosome, and is a C to G SNP mutation, and is related to the phenotype of the cucumber fruit top shape. A KASP primer group is designed through the SNP marker, and the KASP primer group comprises CsFAS-FAM, CsFAS-HEX and CsFAS-COMMON, and the cucumber fruit top shape can be accurately identified through PCR amplification and genotyping technology. The molecular marker can be widely applied to molecular assisted selection of the fruit top shape in cucumber breeding, and has high accuracy and efficiency. In the cucumber breeding, the technology can be screened through genotyping at an early stage, avoids uncertainty and time cost problems existing in traditional phenotype identification, and provides a simple and efficient tool for the cucumber breeding. The technical method of the application is simple and rapid, can be popularized and applied in the cucumber breeding, and has important economic value and social value.

The application provides a CAPS-153 marker and a detection method related to wheat starch content and grain weight, and belongs to the technical field of genotyping. The application provides a sequence of a SNP site closely linked to the thousand-grain weight trait of wheat grains, and provides three SNPs sites on a precursor sequence of tae-miR1121-2A, and five SNPs sites in a promoter region, and the eight SNPs can be closely linked to form two haplotypes Hap1 and Hap2 related to the thousand-grain weight. The application develops a CAPS functional marker to distinguish the wheat haplotypes Hap1 and Hap2, and Hap2 is an excellent haplotype. Therefore, the CAPS functional marker can distinguish the two haplotype wheat varieties of tae-miR1121-2A, and can be applied to molecular marker assisted selection breeding of new wheat varieties with high starch content in the future.

The invention is applicable to the technical field of molecular biology, and provides an InDel marker and a primer group for identifying species of sika deer and red deer and application of the InDel marker and the primer group, the InDel marker is located at a 6116106 site of a fifth chromosome NC057819.1 of the red deer, 120bp of a DNA sequence shown in a sequence table SEQ ID NO: 1 is deleted at the site, and the DNA sequence exists in the sika deer. The invention also provides a primer group for identifying species of sika deer and red deer. The primer group comprises an upstream primer and a downstream primer which are designed aiming at the InDel marker. Based on high-throughput whole genome data of the sika deer and the red deer, the interspecific InDel marker is screened, a corresponding genetic typing technology is established, a technical system which is efficient in detection, low in cost and suitable for large-scale sample screening is constructed, accurate identification of the sika deer, the red deer and interspecific hybrid individuals can be achieved, and better application value is achieved.

The invention provides a CAPS-153 marker related to wheat starch content and grain weight and a detection method, and belongs to the technical field of genetic typing. The invention provides a sequence containing SNP (Single Nucleotide Polymorphism) sites closely linked with the thousand seed weight character of wheat grains, and provides that three SNPs sites exist on a precursor sequence of tae-miR111-2A, five SNPs sites exist in a promoter region, and the eight SNPs can be closely linked to form two haplotypes Hap1 and Hap2 related to the thousand seed weight. The invention develops a CAPS functional marker for distinguishing wheat haplotypes Hap1 and Hap2, and the Hap2 is an excellent haplotype. Therefore, the CAPS functional marker disclosed by the invention can be used for distinguishing two haplotype wheat varieties of tae-miR11121-2A, and can be subsequently applied to molecular marker-assisted selective breeding of new wheat varieties with high starch content.

The application discloses a SNP molecular marker related to a Ningxiang pig canal and application thereof. The molecular marker comprises SNP1, SNP2 and SNP3; the SNP1 molecular marker is located at the 30133410th position of chromosome 7 with the Sus Scrofa Build11.1 genome as a reference genome, the polymorphism is A / G, and the SNP1 molecular marker is numbered as rs327727530; the SNP2 molecular marker is located at the 30263336th position of chromosome 7 with the Sus Scrofa Build11.1 genome as a reference genome, the polymorphism is G / A, and the SNP2 molecular marker is numbered as rs319926630; and the SNP3 molecular marker is located at the 30292654th position of chromosome 7 with the Sus Scrofa Build11.1 genome as a reference genome, the polymorphism is G / A, and the SNP3 molecular marker is numbered as rs341689410. The application identifies the SNP molecular marker significantly related to the Ningxiang pig canal, constructs a genotyping technology system suitable for high-throughput KASP detection, can realize rapid and accurate identification and large-scale screening of the Ningxiang pig PC traits, and significantly improves the breeding efficiency of excellent foraging trait individuals.