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Mutation site associated with total litter size character of sow and application of mutation site

A technology of mutation sites and total litter size, applied in the field of molecular biology, to speed up breeding and improve the core competitiveness of the seed industry

Active Publication Date: 2022-08-09
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no mutation sites associated with reproductive traits in sows have been found on the miR-23a gene

Method used

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  • Mutation site associated with total litter size character of sow and application of mutation site
  • Mutation site associated with total litter size character of sow and application of mutation site
  • Mutation site associated with total litter size character of sow and application of mutation site

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Experimental program
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Effect test

Embodiment 1

[0023] 1. Experimental materials

[0024] Ear samples were randomly collected from large white sows (n=346) in a certain pig farm with ear size forceps, and 1076 records of total litter size were collected.

[0025] 2. Genomic DNA extraction

[0026] DNA was extracted by phenol / chloroform extraction and diluted to 50ng / μL as a PCR amplification template.

[0027] 3. Primer design

[0028] According to the obtained porcine miR-23a gene core promoter (SEQ ID NO.1), use Primer Primer5.0 software to design a pair of primers, and the forward primer sequence is: 5'-CTG CCC TTC AGG CTT CTA GG-3' ( SEQ ID NO.2), the reverse primer sequence is: 5'-TCC CTT CCC CAC CAC CAC T-3' (SEQ ID NO.3). Primers were synthesized by Nanjing Qingke Biotechnology Co., Ltd.

[0029] 4. PCR amplification

[0030] The PCR amplification system is 10 μL, mainly including: 0.5 μL DNA template, 0.5 μM forward primer, 0.5 μM reverse primer, 5 μL 2×Premix rTaq DNA polymerase (TaKaRa company), and make up t...

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Abstract

The invention discloses a pig miR-23a gene core promoter sequence and a mutation site obviously associated with the total litter size character of a sow, the site is located at the 261 basic group position of the pig miR-23a gene core promoter (namely the 65307715 nucleotide position of a second chromosome reference genome), and the site is Cgt; the total litter size of the TT type multiparous sow is higher than that of CC type and CT type sows. The total litter size character is a low-heritability breeding character, the genetic progress is slow by adopting a conventional breeding technology, and as the mutation site is obviously associated with the total litter size character, the high-yield sows can be selected and reserved by detecting the site by adopting a simple and convenient genetic typing technology; the method has important values for accelerating the breeding of high-yield female parent new strains, early completing the localized breeding goal of large white pigs, improving the core competitiveness of the live pig breeding industry in China and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a porcine miR-23a gene core promoter sequence, a mutation site significantly correlated with the traits of the total litter size of multiparous sows, and applications thereof. Background technique [0002] The litter size is a reproductive trait with low heritability and an important economic trait. The litter size is one of the key factors affecting the economic benefits of pig breeding. It is well known that for low-heritability traits, conventional breeding techniques are used for selection, and genetic progress is relatively slow, while molecular breeding techniques based on genetic markers (such as marker-assisted selection breeding, genome selection breeding, and gene editing breeding) are the solution to this problem. A feasible approach to the problem. However, the prerequisite for molecular breeding is to have a large number of reliable genetic markers. Therefore, the ide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12Q1/6888C12N15/11
CPCC12N15/113C12Q1/6888C12N2310/141C12Q2600/156C12Q2600/124Y02P60/87
Inventor 李齐发王思琪杜星吴望军增强
Owner NANJING AGRICULTURAL UNIVERSITY
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