Method for rapid and accurate selective breeding of three-line rice sterile lines by rice genomics technology
A genomics and sterile line technology, which is applied in the field of rapid and accurate breeding of three-line rice sterile lines using rice genomics technology, can solve the problems of long age, unclean recovery gene deletion, insufficient aggregation of comprehensive excellent traits, etc. Speed up breeding and reduce blindness effects
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Embodiment 1
[0024] This example provides a method for improving "Zhong 9A", specifically using rice genomics technology to quickly and accurately select a three-line rice sterile line, including the following steps:
[0025] (1) hybridization
[0026] The maintainer line "Zhong 9B" corresponding to "Zhong 9A" with a high outcrossing rate was used as the female parent 1, and another restorer line "YR0822" with excellent agronomic traits, containing the blast resistance Pi2 gene, and good quality was used as the male parent 1. After conventional sexual crossing, backcross with male parent 1 once, and the obtained BC1F1 generation is self-crossed again to obtain BC1F2 generation segregation population.
[0027] (2) Screening of field agronomic traits
[0028] The BC1F2 generation segregation population was screened for agronomic traits in the field, and 139 excellent individual plants with better plant and leaf morphology were selected, and these 139 excellent individual plants were continu...
Embodiment 2
[0051] This example provides a method for improving the red lotus-type CMS line "Yuetai A", which specifically uses rice genomics technology to quickly and accurately select a three-line rice CMS line, including the following steps:
[0052] (1) hybridization
[0053] The "Yuetai B" maintainer line corresponding to "Yuetai A" was used as the female parent 1, and another restorer line "Wushan Simiao" with excellent agronomic characteristics, containing the rice blast resistance Pi2 gene, and high quality was used as the male parent 1. The parent 1 was backcrossed with the male parent 1 after crossing the male parent 1, and the F2 generation segregation population was obtained, a total of 1297 strains;
[0054] (2) Screening of field agronomic traits
[0055] The field agronomic traits screening was carried out on the F2 generation segregation population, and 131 excellent individual plants with better plant and leaf morphology were selected, and the screening was continued amo...
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