Method for selecting and breeding three-line rice restoring line by rice genomic technology rapidly and accurately
A technology of genomics and restorer lines, applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problems of long-term breeding of three-line restorer lines, insufficient aggregation of comprehensive good traits, etc., to reduce blindness, The effect of shortening the improved generation and speeding up the breeding speed
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Embodiment 1
[0023] This example provides a rapid and accurate breeding of rice with more excellent traits by using the whole genome technology of rice, using "Huanghuazhan" with better plant type, fertilizer tolerance, lodging resistance and high seed setting rate as the parent to be improved The method of restoring new varieties, its technical roadmap is as follows: figure 1 shown, including the following steps:
[0024] (1) hybridization
[0025] "Huanghuazhan" was used as the female parent, and the restorer line "Huazhan" with excellent agronomic traits, early maturation, rice blast resistance Pi2 gene, and good quality was used as the male parent. The male parent was backcrossed once, and the obtained BC1F1 generation was selfed again to obtain 1200 BC1F2 segregation populations.
[0026] (2) Screening of field agronomic traits
[0027] The BC1F2 generation segregation population of step (1) was screened for field agronomic traits, and 126 excellent individual plants with better pl...
Embodiment 2
[0049] This example provides a rapid and accurate selection of rice with more excellent traits by using the rice genome-wide technology and using "9311" with better plant type, fertilizer tolerance, lodging resistance and high seed setting rate as the parent to be improved The method of restoring new varieties, its technical roadmap is as follows: figure 1 shown, including the following steps:
[0050] (1) hybridization
[0051] "9311" was used as the female parent, and the restorer line "YR0822" with excellent artistic traits, precocity, rice blast resistance Pi2 gene, and good quality was used as the male parent. This backcross was once, and the BC1F1 generation obtained was self-crossed again, and 1100 BC1F2 isolated populations were obtained.
[0052] (2) Screening of field agronomic traits
[0053] The BC1F2 segregation population of step (1) was screened for agronomic traits in the field, and 119 excellent individual plants with better plant and leaf morphology were s...
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