Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of molecular marker indel6 related to oil content and its application

A molecular labeling and labeling technology, which is applied in the direction of biochemical equipment and methods, DNA/RNA fragments, microbial determination/inspection, etc., can solve the problem of low accuracy, achieve heavy workload, overcome time constraints, and long cycle Effect

Active Publication Date: 2020-01-07
HENAN AGRICULTURAL UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, with the development of molecular marker technology, many researchers have used markers linked to oil-related QTLs to perform assisted selection on soybean germplasm. This method improves the speed of selection to a certain extent and is easy to operate, but These markers are linkage markers of soybean oil gene, so the accuracy is generally low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of molecular marker indel6 related to oil content and its application
  • A kind of molecular marker indel6 related to oil content and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Test materials

[0026] Using 15 soybean core germplasms with different oil content provided by the National Soybean Improvement Center, including 6 high-oil germplasms, 7 low-oil germplasms and 2 medium oil content germplasms, as shown in Table 1:

[0027] Table 1. 15 soybean germplasms with different oil content

[0028] Numbering Material Oil content (%) Oil content 1 Nannong 1138-2 17.52 Low / L 2 Zhou Bean 29 17.25 Low / L 3 Soviet Union 1 17.18 Low / L 4 Mid Bean 14 16.91 Low / L 5 Nannong 94-156 17.83 Low / L 6 Yudou 16 16.79 Low / L 7 wave height 18.52 Medium / M 8 spring beans 18.04 Medium / M 9 Shangqiu 73 17.19 Low / L 10 Kefeng 1 20.25 High / H 11 Zhou Dou 94010 19.95 High / H 12 Jianli Beef Hair Yellow 21.96 High / H 13 Millstone Soy 22.12 High / H 14 Anyi Black Bean 20.66 High / H 15 Xiping brown noodles 21.3 High / H

[0029] Th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a functional molecular marker Indel6 for oil related characters and application of the molecular marker, and relates to the technical field of molecular markers. In the functional molecular marker Indel6 for the oil related characteristics, the oil related gene GmOLEO1 is located at the 20th chromosome of soybean, and the CDS sequence of the oil related gene GmOLEO1 is shownin the SEQ ID NO.1. The marker Indel6 can be used for parting and identifying a new oil related material, and used for verifying soybean germplasms which are obtained through a common chemical methodin the earlier stage and are different in oil content, and the accuracy reaches 85% or above. Compared with a common screening method, time and labor are saved, the cost is reduced, the method is very high in reliability, and a new material and technology are provided for further oil related gene type germplasm verification and assistant selection of the molecular marker for soybean variety breeding.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to an oil-related gene molecular marker Indel6 and its application. Background technique [0002] Soybean is an important food crop and oil crop in my country. But the current dependence on foreign countries has reached over 85%. Therefore, exploring the genetic potential of soybean itself and cultivating new high-oil soybean varieties through molecular breeding and other technical means is an effective way to solve the above problems. [0003] Although a lot of progress has been made in soybean oil-related research, there are still some difficulties in the screening of different oil genotypes, mainly because the oil content must be measured with soybean grain samples, which are seasonal and oil content The content will undergo changes such as degradation when the grain sample is not stored properly, so the determination of large groups is not only time-consu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 张丹董中东刘伟马兴立李忠锋
Owner HENAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products