14 results about "Genomic technology" patented technology

The invention relates to the technical field of animal genetic breeding and genomics, and discloses a reference genome of a chromosome level of a Zhaotong Wujin pig and application of the reference genome. The high-quality chromosome level reference genome of the Zhaotong Wujin pig is constructed for the first time. The total length of the genome is about 2.66 Gb, the chromosome anchoring rate is more than 98%, Contig N50 is about 102Mb, and the integrity evaluation of the genome is more than 98%; 21507 protein coding genes are annotated in total. Compared with the existing reference sequence, the genome disclosed by the invention contains specific sequence variation and gene family information of local pig breeds, and provides a more accurate and comprehensive genetic blueprint. The genome resource can be widely applied to molecular mechanism research related to traits such as meat quality, reproductive capacity, growth speed, disease resistance and the like, molecular marker development, breeding chip design, quantitative trait locus (QTL) positioning, genetic evaluation, gene editing, transgenic breeding, germplasm resource protection and the like. The method has important application value in genetic improvement of Chinese local pig breeds.

The invention discloses salt-resistant and alkali-resistant xylanase and application thereof, and relates to the technical field of biology. The amino acid sequence of the salt-resistant and alkali-resistant xylanase is as shown in SEQ ID NO: 2. According to the invention, a novel salt-resistant and alkali-resistant gene cwp6-xyn10 is obtained from the Qaihu saline-alkali soil by utilizing a metagenome technology. Then, the gene is subjected to heterologous expression in E.coli, and enzymatic properties are analyzed. Enzymatic characterization shows that the CWP6-Xyn10 is a salt-resistant, alkali-resistant and high-activity xylanase, and has good salt resistance and pH stability.

The invention discloses thermoacidophilic beta-glucosidase and application thereof, and relates to the technical field of biological enzymes. The thermoacidophilic beta-glucosidase is BGL74, and the amino acid sequence of the thermoacidophilic beta-glucosidase is as shown in SEQ ID NO: 4. According to the invention, the GH1 family beta-glucosidase gene bgl74 is screened and cloned by adopting a metagenome technology from the bottom mud of a hot spring of Longwang temple from Dali Pu'er source in Yunnan province, heterologous expression is realized in escherichia coli, and the recombinase BGL-74 is purified. Research results show that the BGL-74 has the characteristics of heat resistance, acid resistance, glucose resistance and the like, has good affinity and catalytic efficiency on soybean isoflavone glycoside, and verifies the feasibility and application prospect of obtaining high-performance beta-glucosidase through hot spring metagenome mining to promote soybean isoflavone aglycone. And a high-quality biological catalysis tool is provided for functional food development and biological manufacturing.

The application provides a chitosanase and application thereof, and relates to the technical field of enzyme engineering. The amino acid sequence of the chitosanase is shown as SEQ ID NO. 1. The chitosanase prepared by the application can efficiently and controllably catalyze chitosan hydrolysis to generate chitooligosaccharide with a specific polymerization degree. The enzyme is obtained by screening from an extreme environment through metagenomic technology, has a novel sequence, unreported catalytic characteristics and can precisely regulate product composition, and meets the preparation requirements of specific high-purity chitooligosaccharide. These characteristics make the enzyme have important application value in the large-scale production of chitooligosaccharide, provide an effective way for solving the problems of single product and low purity in the traditional method, and lay a technical foundation for the development in the fields of medicine, food and agriculture.

The invention provides novel chitosanase and application thereof, and relates to the technical field of enzyme engineering. The amino acid sequence of the chitosanase is as shown in SEQ ID NO. 1. The novel chitosanase prepared by the invention can efficiently and controllably catalyze chitosan hydrolysis to generate chitosan oligosaccharide with a specific polymerization degree. The enzyme is obtained by screening from an extreme environment through a metagenome technology, is novel in sequence, has unreported catalytic characteristics, can accurately regulate and control product composition, and meets the preparation requirements of specific high-purity chitosan oligosaccharide. Due to the characteristics, the enzyme has important application value in large-scale production of chitosan oligosaccharide, an effective way is provided for solving the problems of single product and low purity in the traditional method, and a technical foundation is laid for development of the fields of medicine, food and agriculture.

The invention provides a local livestock and poultry traceability method based on an SNP marker, and relates to the field of variety traceability, and the method comprises the steps of traceability gene bank construction, genetic relationship determination, traceability identification based on genome data, and traceability identification process and result determination. Wherein the traceability gene pool construction comprises source gene pool sample collection, sample collection and information recording, gene analysis and data generation, and traceability gene pool establishment; the genetic relationship judgment comprises repeated individual identification and paternity test; the traceability identification based on the genome data comprises repeated individual identification based on genome information and parent-child relationship identification based on the genome information; the traceability identification process and result judgment comprises sample receiving and quality control, data comparison and analysis and hierarchical result judgment. According to the method, SNP marker information is obtained through a genomics technology, accurate source tracing of livestock and poultry meat products is achieved through genetic relationship analysis, and the counterfeit and shoddy phenomena are effectively eradicated.

The invention discloses an efficient corn drought-resistant breeding method integrating molecular marker development and a whole genome prediction model. The efficient corn drought-resistant breeding method comprises the steps of hybridization matching and DH line induction, multi-environment phenotype and genotype analysis, three-level marker set screening, optimal prediction system construction, excellent selfing line screening and the like. The method has the remarkable effects that a whole genome selection technology is applied to identification and screening of the saline-alkali-tolerant and drought-tolerant corn materials, a whole genome breeding process for selecting the saline-alkali-tolerant and drought-tolerant materials is established, and generation intervals are shortened through early selection, so that the breeding value estimation accuracy is improved, the genetic progress is accelerated, and the breeding efficiency is improved. And the method has a good prediction effect on low-heritability and difficult-to-measure complex characters, and truly realizes the breeding practice guided by a genome technology.

The application provides a chitosanase and application thereof, and relates to the technical field of enzyme engineering. The amino acid sequence of the chitosanase is shown as SEQ ID NO. 1. The novel chitosanase prepared by the application can efficiently and controllably catalyze chitosan hydrolysis to generate chitooligosaccharide with a specific polymerization degree. The enzyme is obtained by screening from an extreme environment through metagenomic technology, has a novel sequence, unreported catalytic characteristics and can precisely regulate product composition, and meets the preparation requirements of specific high-purity chitooligosaccharide. These characteristics make the enzyme have important application value in the large-scale production of chitooligosaccharide, provide an effective way for solving the problems of single product and low purity in the traditional method, and lay a technical foundation for the development in the fields of medicine, food and agriculture.

The invention discloses a method for constructing a chromosome three-dimensional structure based on fusion gene data and application of the method, and belongs to the technical field of genomes. According to the method, internal interaction of fusion genes and interaction between chromosomes are analyzed, hierarchical structures of second, third and fourth stages of the chromosomes are determined by calculating the frequency of the interaction, and a three-dimensional structure is constructed according to the hierarchical structures of the second, third and fourth stages of the chromosomes; the method is of great significance in exploring gene interaction and determining the relation between the chromosome spatial position and the disease.

This invention discloses a multi-omics joint detection method in the field of microbiology and fermentation technology for Baijiu brewing to analyze regional heterogeneity of high-temperature Daqu (fermentation starter). Taking high-temperature Daqu from different production areas and strata as examples, it integrates physicochemical enzyme activity assays, electronic sensory analysis, volatileomics, microbial amplicon sequencing, metagenomics, and metagenomic assembly technologies to systematically characterize the differences in physicochemical properties, sensory flavors, microbial communities, and metabolic functions of samples. It establishes a correlation model of "phenotype, flavor, microbial community, function, and strain," clarifying the impact of production area and stratum on Daqu quality and elucidating the mechanism of regional heterogeneity formation in high-temperature Daqu. This invention elevates the analytical depth from the genus level to the species and genome level, accurately locating core functional strains. It solves the problems of traditional methods having single detection dimensions, shallow analytical depth, and unclear functional attribution, providing scientific methods and theoretical support for improving the quality of high-temperature Daqu in Maotai-flavor Baijiu and enhancing the flavor of non-core production areas.

The application discloses a method for matching a metagenome queue based on a microbial metabolic background and relates to the technical field of metagenomics.S1: metagenome sequencing data processing; standardizing processing of data from whole metagenomics and manually input meta data;S2: extraction of a main metabolic background of microorganisms;S3: matching of the metabolic background of microorganisms; firstly, a nearest neighbor matching algorithm is used to screen matched samples in a control group without missing any main metabolic components;S4: matching effect checking; balance test is conducted on the mean of covariants of the matched disease group and the control group;S5: difference analysis based on the matched queue; if the data of the matched disease group and the control group meet normal distribution, paired sample t test is conducted for difference analysis, otherwise, group wilcoxon test is used for difference analysis.Through the technical method, a matched queue of case and control samples in microbial research is constructed, and the causal relationship identification capability of metagenomics research is strengthened.

The invention discloses a method for detecting rhizosphere and non-rhizosphere microbial communities of bluegrass based on a metagenome technology, and relates to the technical field of molecular biology, and the method comprises the following steps: (1) sample collection, (2) soil DNA extraction: circularly grinding a sample through freezing-pyrolysis, cracking with a CTAB buffer solution at 65 DEG C, carrying out trichloromethane extraction, removing RNA from RNase, and carrying out ethanol precipitation and crude extraction; (3) DNA purification: treating with GDP, purifying with an adsorption column, and eluting with a preheated elution buffer solution; and (4) metagenome sequencing and community analysis are carried out, so that the DNA extraction problem caused by high content of inhibitors such as humic acid and polysaccharide in the soil in the alpine region and special microbial cell wall structures is effectively solved, and the DNA yield, purity and integrity are improved through an optimized splitting decomposition and purification strategy; and technical support is provided for extraction of metagenomes and analysis of microbial community structures in extreme environments such as alpine and the like.

The application provides a local livestock and poultry traceability method based on SNP markers, relates to the field of breed traceability, and comprises traceability gene library construction, kinship determination, traceability identification based on genomic data, and traceability identification process and result determination; wherein, the traceability gene library construction comprises source gene library sample collection, sample collection and information recording, gene analysis and data generation, and traceability gene library establishment; the kinship determination comprises repeated individual identification and parent-offspring identification; the traceability identification based on genomic data comprises repeated individual identification based on genomic information and parent-offspring relationship identification based on genomic information; the traceability identification process and result determination comprises sample receiving and quality control, data alignment analysis, and hierarchical result determination. The method realizes accurate traceability of livestock and poultry meat products by obtaining SNP marker information through genomics technology and realizing accurate traceability of livestock and poultry meat products by using kinship analysis, and effectively prevents false and inferior phenomena.

This invention discloses a method for preparing a 10K SNP liquid-phase microarray for commercial pig breeds and its application, belonging to the field of molecular breeding and genomics technology. Based on representative samples from the core breeding population of Chinese commercial pigs, this invention employs liquid-phase probe capture technology to develop a high-performance pig genome SNP genotyping microarray containing 10,055 rigorously screened high-quality SNP loci. The probes are 110 bp in length, with a GC content of 30%–70% and ≤5 homologous regions. Each locus is captured using two probes with 60%–70% overlap for dual targeting, significantly improving hybridization efficiency and anti-interference ability, and enabling stable detection of low-quality DNA samples. Simultaneously, this invention sets dual quality control standards to ensure genotyping accuracy. Verification shows that the detection rate of this microarray ranges from 98.17% to 99.602%, with an average detection rate of 99.161%. This invention has significant value for improving the breeding efficiency of commercial pigs in my country.