52 results about "Genomic technology" patented technology

Methods and compositions for determining a methylated cytosine profile of a target nucleic acid sequence are provided.

Embodiments relate to genomic technologies using adaptive spline analysis that predict responses of cancer cells. For example, responses of cancer cells to specific medications and / or treatments may be predicted based on adaptive linear spline analyses.

The invention discloses a method for rapid and accurate selective breeding of three-line rice sterile lines by a rice genomics technology. By assisted screening combination of conventional breeding with molecular markers, the method can effectively eliminate fertility restoring genes, and genetic background screening is performed by a genotyping technique based on rice whole genome sequencing, andsterile lines and corresponding maintainer lines having genetic background closer to target parents can be screened. Compared with the prior art, the method greatly shortens improved generations of the maintainer lines and backcross generations of the sterile lines, and the three-line rice sterile lines with excellent comprehensive characters can be accurately selectively bred within only 4-5 years.

The invention discloses a method for quickly and accurately selecting a three-line rice sterile line by using rice genomics technology. The method combines conventional breeding with molecular marker-assisted screening to effectively eliminate fertility restoration genes, and at the same time utilizes the method based on the whole genome of rice Genetic background screening by sequencing genotyping technology can screen and obtain sterile lines and corresponding maintainers whose genetic background is closer to the target parent. Compared with the existing technology, this method greatly shortens the improved generation of the maintainer line and the backcross generation of the sterile line, and can accurately breed a three-line rice sterile line with excellent comprehensive characteristics in only 4-5 years.

The invention discloses a method for eliminating three-dimensional genomics technology noise by utilizing exonuclease combination. The method comprises the step of adding exonuclease combination to eliminate adjacently connected linear DNA when performing the adjacent connection at the key step of the three-dimensional genomics technology, thereby improving the cyclic effective interaction proportion. The excellent performance of the exonuclease combination for eliminating the linear DNA provides an effective measure for eliminating the noise through the enzyme digestion of the exonuclease combination after the C-technology connection; the method for eliminating the three-dimensional genomics technology noise by utilizing exonuclease combination has the advantages of being convenient, fastand efficient, the possibility of producing the false-positive product or signal can be reduced, the effective interaction cyclic DNA proportion is enhanced, the terminal effective interaction data output ratio is greatly improved, the sequencing space is saved, the sequencing cost is reduced, and the operation for eliminating the resource consumed by ineffective information in the big data by using the complex biological information at later is simplified.

An agriculture management and analysis system for analysis, interpretation, and visualization of genetic data to improve production, performance, and management of agriculture, such as livestock.

The invention discloses a method for selecting and breeding a three-line rice restoring line by a rice genomic technology rapidly and accurately. According to the method, the conventional breeding iscombined with molecular marker auxiliary screening, genetic background screening is conducted by a genotyping technology based on rice whole genome sequencing, and the restoring line which contains ahomozygous sterility restoring gene and has the genetic background closer to a target parent can be screened and obtained. Compared with the prior art, the method has the following advantages: the improved generation of the restoring line is greatly shortened, and the three-line rice restoring line with excellent comprehensive characters can be selected and bred accurately for only 3 to 4 years.

The invention relates to novel metal ion tolerance keratinase and application thereof, and provides keratinase based on metagenome technology excavation and an application method of the keratinase, belonging to the field of industrial biotechnology. The overall length of the keratinase is 1,149bp, and 382 amino acids are encoded; take B.subtilis WB600 as an example, the heterologous expression ofa keratinase gene is successfully realized. The method for obtaining the keratinase gene is convenient and feasible, the gene is subjected to recombined expression, a constructed recombination straincan realize secretory expression of recombined keratinase, the recombined keratinase has relatively good tolerance to metal ions and a surface active agent, and the stability of enzyme is relatively good. Recombinant bacteria are short in fermentation period and suitable for industrial production in large scale. In addition, the enzyme has good reducing capacity and can be applied to research in which a biological method is used for preparing nano-silver particles, and the prepared nano-silver particles have good forms and relatively good bacteriostatic activity.

The invention discloses a susceptible gene for acute exacerbation of chronic obstructive pulmonary disease and application of the susceptible gene in prediction of susceptible acute exacerbation of chronic obstructive pulmonary disease. Clinical specimens such as blood of a patient in the chronic obstructive pulmonary disease acute exacerbation stage are collected, blood circulation DNA whole exons of the COPD patient are detected through a genomics technology, and 20 AECOPD specific susceptibility genes are obtained by comparing the gene difference of the whole exons of the chronic obstructive pulmonary disease patient in the stable stage and the acute exacerbation stage; through gene detection of a verification queue, a highly specific gene for predicting acute exacerbation of chronic obstructive pulmonary disease is identified in the 20 genes, and can be used for screening susceptible patients with acute exacerbation of chronic obstructive pulmonary disease, so that a basis is provided for researching and verifying prognosis and recurrence markers of susceptible acute exacerbation of chronic obstructive pulmonary disease in a larger clinical sample in the future; wide application prospects are realized.

The application belongs to the field of plant genome technology, and in particular relates to the vinyl protein receptor Ab143539 in the Agaricus bisporus genome. There are five transmembrane regions in its structure, and a cysteine ​​residue is contained in the third transmembrane region. The specific base sequence is shown in SEQ ID NO.1. Based on the results of structural comparison, the inventor speculated that Ab143539 is the ethylene receptor protein (AbEBD2) in Agaricus bisporus, which can bind ethylene. After further practical verification, it was found that the protein has ethylene binding ability, but its binding ability is significantly weaker than that of the Arabidopsis ethylene receptor ETR1. However, based on this research result, through genetic engineering technology, it can still lay a certain technical foundation for the cultivation of new varieties of Agaricus bisporus, or technical improvements such as Agaricus bisporus preservation.

The invention belongs to the field of water ecological safety, and particularly relates to a method for identifying pathogenic bacteria carrying toxic factors in a water body based on a metagenome technology. According to the method, through a metagenome sequencing technology and a gene assembling method, the types and the number of the toxic factors in a water body are determined on the gene level, and pathogen host information of the toxic factors is identified, so that pathogenic bacteria carrying the toxic factors in the water body and potential health risks of the pathogenic bacteria aredetermined. The method has the characteristics of high flux, quickness, simplicity and convenience, and is suitable for large-scale sampling investigation and research.

The invention provides SSR (simple sequence repeat) molecular marker primers and method for identifying scutellaria baicalensis in the northeast region, and the method comprises the following steps: taking total DNA (deoxyribonucleic acid) of a scutellaria baicalensis sample to be identified as a template, and performing PCR (polymerase chain reaction) amplification by using the SSR molecular marker primers to obtain an amplification product; performing capillary electrophoresis sequencing typing on the amplification product to obtain a typing result of the amplification product; carrying out peak map interpretation on the typing result of the amplification product by using GeneMarker 4.0 to obtain an allele matrix; according to the allele matrix, carrying out GenALEx calculation to obtain genetic diversity data of the scutellaria baicalensis; and on the basis of genetic diversity data obtained by GenALEx calculation, carrying out UPGMA clustering analysis through MEGA to construct a clustering tree. A genome technology and an SSR molecular marker technology are applied, the SSR primers are designed by utilizing the scutellaria baicalensis genome, the SSR primers are screened through scutellaria baicalensis samples in Hebei, Shanxi, Shandong, Shaanxi, Inner Mongolia, Liaoning, Jilin and the like, and the primers are used for specifically identifying scutellaria baicalensis samples in the northeast region, are high in accuracy and stability and are easy to popularize and apply in a large scale.

The invention discloses genes and proteins for prosthetic group-free chloroperoxidase CPO822 with multiple functions, particularly capability of degrading benzopyrene and having activities of peroxidase, catalase, halogenase and esterase, and application of the genes and proteins. The invention has the beneficial effects that by virtue of a metagenome technology, a novel DNA sequence without functional study is obtained from soil metagenome; study results of the structures and functions of proteins coded by the DNA sequence show that a recombinant protein expressed by the DNA sequence has polyfunctionality, has a wider catalytic reaction range than existing separated peroxidase, can serve as a novel enzyme source to be applied to the fields such as washing, wastewater treatment, disinfection, lignin removal, production of epoxy derivatives, degradation of benzopyrene and the like, and has wide industrial application prospects. In addition, the study discovers that the enzyme is stableunder a wide-range pH value, is high in stability at a high temperature, and has great significances on wide popularization and application.