The invention provides SSR (simple
sequence repeat)
molecular marker primers and method for identifying
scutellaria baicalensis in the northeast region, and the method comprises the following steps: taking total
DNA (deoxyribonucleic acid) of a
scutellaria baicalensis sample to be identified as a template, and performing PCR (
polymerase chain reaction) amplification by using the SSR
molecular marker primers to obtain an amplification product; performing
capillary electrophoresis sequencing
typing on the amplification product to obtain a
typing result of the amplification product; carrying out peak map interpretation on the
typing result of the amplification product by using GeneMarker 4.0 to obtain an
allele matrix; according to the
allele matrix, carrying out GenALEx calculation to obtain
genetic diversity data of the
scutellaria baicalensis; and on the basis of
genetic diversity data obtained by GenALEx calculation, carrying out UPGMA clustering analysis through
MEGA to construct a clustering tree. A
genome technology and an SSR
molecular marker technology are applied, the SSR primers are designed by utilizing the
scutellaria baicalensis genome, the SSR primers are screened through
scutellaria baicalensis samples in Hebei, Shanxi, Shandong, Shaanxi, Inner Mongolia, Liaoning, Jilin and the like, and the primers are used for specifically identifying
scutellaria baicalensis samples in the northeast region, are high in accuracy and stability and are easy to popularize and apply in a large scale.