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A gene mutation site for weeding out low fecundity Hu sheep and its application

A technique for fecundity, Hu sheep, applied in the field of molecular biology

Active Publication Date: 2021-12-10
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although scientists have discovered a number of major genes (such as BMP15, BMPR1B, GDF9, Woodlands and B4GALNT2 genes) that control sheep reproductive traits (mainly including lamb size and ovulation number) in foreign sheep breeds, a total of 15 genes have been identified. SNPs loci, but except the FecB locus of BMPR-IB gene exists in Hu sheep, other loci are not related to the high fecundity traits of Hu sheep

Method used

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  • A gene mutation site for weeding out low fecundity Hu sheep and its application
  • A gene mutation site for weeding out low fecundity Hu sheep and its application
  • A gene mutation site for weeding out low fecundity Hu sheep and its application

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Embodiment 1

[0022] 1. Experimental materials

[0023] Choose healthy Hu sheep ewes with no disease and good body condition, and record the number of lambs born. Ear tissue samples were collected with ear size forceps and stored at -20°C for extraction of genomic DNA.

[0024] 2. Genomic DNA extraction

[0025] Genomic DNA was extracted from ear tissues of Hu sheep by conventional phenol / chloroform extraction method and stored at -20°C.

[0026] 3. Primer design

[0027] According to the sheep genome sequence (GenBank ID: NC_019463.2), use Primer5.0 software to design a pair of primers, the forward primer sequence is: 5'-TGT GTA GGA AGT TGA ACC CCT-3' (SEQ ID NO.1), The reverse primer sequence is: 5'-GAA TAA GGT TGA GAG GGA TGT GT-3' (SEQ ID NO.2). Primers were synthesized by Nanjing Qingke Biotechnology Co., Ltd.

[0028] 4. PCR amplification

[0029] The PCR amplification system is 10 μL, mainly including: 0.5 μL Hu sheep genomic DNA, 0.5 μM forward primer, 0.5 μM reverse primer, 5...

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Abstract

The invention discloses a gene mutation site for eliminating low fecundity Hu sheep and its application. The mutation site is located at the 1354th nucleotide of the 3'-UTR of Hu sheep BMPR1B gene, and the site is G>A The average number of lambs per litter in GA-type multiparous Hu sheep ewes was 0.54 lower than that of GG-type individuals, and the transcriptional activity of BMPR1B gene 3'‑UTR of A-type was only half that of G-type. The reproductive trait of livestock is a low heritability trait, and the genetic progress of conventional breeding technology is relatively slow. Because the mutation site causes the reproductive performance of multi-bred Hu sheep ewes to be significantly reduced, simple genotyping technology is used to detect this site. The individuals with low fertility genotypes can be eliminated, and the individuals with high fertility genotypes can be selected, which has important economic value in accelerating the progress of breeding of high fertility Hu sheep populations or strains.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a gene mutation site for eliminating low fecundity Hu sheep and its application. Background technique [0002] Hu sheep is a famous sheep breed in my country. It is one of the first 138 national livestock and poultry genetic resource protection breeds by the Ministry of Agriculture, and one of the 42 local sheep breeds or resources included in the Chinese sheep breed catalog. Hu sheep are mainly distributed in the Jiangsu, Zhejiang and Shanghai regions in the Taihu Lake Basin. The central production areas are located in Suzhou City in Jiangsu Province, Huzhou City and Jiaxing City in Zhejiang Province. Hu sheep is also the only sheep breed in southern my country. It has excellent germplasm characteristics such as high-quality lamb skin, estrus in four seasons, early sexual maturity, high multiple births, resistance to humidity and heat, fast early growth, tender and delicious meat, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李齐发杜星姚一龙曹秋瑜
Owner NANJING AGRICULTURAL UNIVERSITY
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