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A method for rapid and precise breeding of three-line rice sterile lines using rice genomics technology

A genomics and rice technology, applied in the field of rapid and accurate selection of three-line rice sterile lines using rice genomics technology, can solve the problems of unclean gene elimination, long life, and insufficient aggregation of comprehensive good traits, etc., to reduce blindness Sex, the effect of speeding up breeding

Active Publication Date: 2021-08-10
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art, and provides a method for rapidly and accurately breeding three-line rice sterile lines using rice genomics technology, aiming at innovating breeding methods and means, and solving the problems of existing three-line rice breeding. The number of years of breeding lines is long, the genetic distance is relatively close, the recovery genes are not cleanly eliminated, and the aggregation of comprehensive good traits is not enough, etc.

Method used

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  • A method for rapid and precise breeding of three-line rice sterile lines using rice genomics technology
  • A method for rapid and precise breeding of three-line rice sterile lines using rice genomics technology

Examples

Experimental program
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Embodiment 1

[0024] This example provides a method for improving "Zhong 9A", specifically using rice genomics technology to quickly and accurately select a three-line rice sterile line, including the following steps:

[0025] (1) hybridization

[0026] The maintainer line "Zhong 9B" corresponding to "Zhong 9A" with a high outcrossing rate was used as the female parent 1, and another restorer line "YR0822" with excellent agronomic traits, containing the blast resistance Pi2 gene, and good quality was used as the male parent 1. After conventional sexual crossing, backcross with male parent 1 once, and the obtained BC1F1 generation is self-crossed again to obtain BC1F2 generation segregation population.

[0027] (2) Screening of field agronomic traits

[0028] The BC1F2 generation segregation population was screened for agronomic traits in the field, and 139 excellent individual plants with better plant and leaf morphology were selected, and these 139 excellent individual plants were continu...

Embodiment 2

[0051] This example provides a method for improving the red lotus-type CMS line "Yuetai A", which specifically uses rice genomics technology to quickly and accurately select a three-line rice CMS line, including the following steps:

[0052] (1) hybridization

[0053] The "Yuetai B" maintainer line corresponding to "Yuetai A" was used as the female parent 1, and another restorer line "Wushan Simiao" with excellent agronomic characteristics, containing the rice blast resistance Pi2 gene, and high quality was used as the male parent 1. The parent 1 was backcrossed with the male parent 1 after crossing the male parent 1, and the F2 generation segregation population was obtained, a total of 1297 strains;

[0054] (2) Screening of field agronomic traits

[0055] The field agronomic traits screening was carried out on the F2 generation segregation population, and 131 excellent individual plants with better plant and leaf morphology were selected, and the screening was continued amo...

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Abstract

The invention discloses a method for quickly and accurately selecting a three-line rice sterile line by using rice genomics technology. The method combines conventional breeding with molecular marker-assisted screening to effectively eliminate fertility restoration genes, and at the same time utilizes the method based on the whole genome of rice Genetic background screening by sequencing genotyping technology can screen and obtain sterile lines and corresponding maintainers whose genetic background is closer to the target parent. Compared with the existing technology, this method greatly shortens the improved generation of the maintainer line and the backcross generation of the sterile line, and can accurately breed a three-line rice sterile line with excellent comprehensive characteristics in only 4-5 years.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for rapidly and accurately selecting a three-line rice sterile line by using rice genomics technology. Background technique [0002] At present, the practice of hybrid rice breeding has proved that rapid and precise breeding of male sterile lines with high combining ability, high rice quality, multi-resistance and good outcrossing habits is the key to breeding strong and superior combinations. Over the years, China's three-line hybrid rice has been widely used in China and the world because of its high and stable yield and relatively safe seed production. It plays a vital role in solving food security problems. However, the breeding of three-line rice male sterile lines is limited by the hereditary traits of the male sterile line being plasmogenic-nuclear interactive sterile, so it is difficult to breed a new quality-sourced maintainer line. Although great progres...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 韩斌张从合黄学辉王慧陈金节蒋家月冯旗严志方玉王阿红黄艳玲
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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