Method for Determining Sequencing Digestion Combination in Sequencing Genotyping Technology

A genotyping and genome technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of affecting the efficiency of enzyme digestion, reducing the density of SNP mining, increasing the cost of experiments, etc.
CN105368930BActive Publication Date: 2018-11-20CHINA AGRI UNIV
4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2018-11-20

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a determining method for sequencing enzyme digestion combination in a sequencing genotyping technology. The determining method comprises the following steps that 1, restriction enzyme digestion site predicting is performed on a target genome, and the number of enzyme digestion segments obtained through different enzyme digestion modes is counted; 2, a joint sequence and a PCR amplification primer sequence at the two ends of each enzyme digestion segment are designed according to the predicted enzyme digestion segments in the various enzyme digestion modes in the step 1; 3, sequencing libraries are constructed through a GBS technology for the different enzyme digestion modes; 4, sequencing is performed through the sequencing libraries constructed in the step 3; 5, SNP marker sites are obtained according to sequencing results; 6, the specific enzyme digestion combination for the target genome is determined according to the number of the SNP marker sites and the enzyme digestion segment sizes which are obtained through different enzyme digestion combinations.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for determining a combination of sequencing restriction enzymes in sequencing genotyping technology. Background technique

[0002] Genetic molecular markers (measurable and inheritable polymorphisms among different individuals within one or more populations) firmly occupy the core position of modern genetics and are also important for disciplines such as population genetics, ecology and developmental biology. research direction. The current mainstream genetic markers have been developed to the third generation, namely single nucleotide polymorphisms (Single Nucleotide Polymorphisms, SNP) molecular markers. This kind of genetic marker is characterized by the substitution of a single base, and generally consists of only two bases. It is a dimorphic marker, and the difference in length between the first-generation RFLP and the second-generation STR is used as a genetic marker....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More