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Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization

A distant hybridization, radish technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problem of lack of systematic research on embryo rescue, and achieve the effect of improving compatibility

Inactive Publication Date: 2011-10-12
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For the distant hybridization of radish and turnip, especially when embryo abortion occurs during the hybrid embryo development stage, there is no systematic research at home and abroad on the impact of the best time, best location, and culture conditions for embryo rescue on embryo rescue.

Method used

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  • Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization
  • Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization
  • Method for achieving isolated embryos through radish and turnip intergeneric distant hybridization

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Embodiment Construction

[0024] The present invention will be described in detail below:

[0025] 1. Materials and methods

[0026] 1.1 Test materials

[0027] The radish male sterile line DH303A was used as the female parent, and the turnip was used as the male parent. Both parents were provided by the Vegetable Science and Technology Center of Hubei Academy of Agricultural Sciences.

[0028] 1.2 Test method

[0029] This experiment was conducted at the Vegetable Science and Technology Center of the Economic Crop Research Institute of Hubei Academy of Agricultural Sciences.

[0030] 1.2.1 Embryo rescue after pod setting

[0031] Pick the pods after 5, 6, and 7 days after pollination, sterilize with 70% ethanol for 0.5 min, then soak in 0.1% HgCl for 8-10 minutes, rinse with sterile water 3 times, soak in sterile water for 3 minutes each time. Before inoculation, dry the sterile water on the surface of the pod with sterile filter paper, peel it off carefully to obtain hybrid embryos, and place hybrid ovules into...

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Abstract

The invention relates to a method for achieving isolated embryos through radish and turnip intergeneric distant hybridization. The method is characterized by sequentially comprising the following steps of: (1) preparing materials; (2) sowing; (3) bagging and hanging plates; (4) performing pollination; and (5) saving embryos. By the method, the radish and turnip intergeneric distant hybridization isolated embryos are obtained successfully; hybrid embryo seedlings are cultivated; and a new breeding resource is obtained.

Description

Technical field [0001] The invention belongs to the field of rapid propagation of plant tissue culture, and specifically relates to a method for obtaining in vitro embryos by distant hybridization between radish and turnip. Background technique [0002] Radish (Raphanus sativus L.) is one of the main vegetable crops cultivated in our country, cruciferous, Radish genus, one or two-year-old herb. It is native to my country and is cultivated everywhere. There are many varieties, such as white radish, red radish and green radish. The root can be eaten fresh or raw. The seeds contain 42% oil and can be used to make soap or lubricating oil. Seeds, fresh roots, and leaves can all be used as medicine, which can eliminate qi under function. Raw radish contains amylase, which can help digestion. Rutabaga (Brassica juncea Coss.var.napiformmis Palet.Bols) is a variety of mustard, which is used for roots. It is a biennial herb of the Cruciferae and Brassica genus. The product organ is fles...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
Inventor 梅时勇甘彩霞崔磊何云启黄来春王晴芳丁自立张兴中梅雪飞高翔
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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