Method for realizing distant hybridization of adzuki beans and rice beans by utilizing young embryo rescue

A technology of immature embryo rescue and distant hybridization, which is applied in the fields of botany equipment and methods, horticultural methods, applications, etc., can solve the problems of backward breeding and genetic research, lack of research reports on distant hybrid breeding, and the absence of adzuki bean and rice bean. In order to achieve the effects of high transplanting survival rate, ensure the rate of hybridization and pod formation, and avoid abortion

Active Publication Date: 2014-12-24
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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Problems solved by technology

However, adzuki bean is a small crop in my country, and its breeding and genetic research is relatively backward. The current main breeding methods are still based on natural variation selectio

Method used

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  • Method for realizing distant hybridization of adzuki beans and rice beans by utilizing young embryo rescue
  • Method for realizing distant hybridization of adzuki beans and rice beans by utilizing young embryo rescue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Obtaining of Example 1 Adzuki Bean and Rice Bean Distant Hybrid Seedlings

[0027] 1. Test materials

[0028] The early-maturing rice bean parent material F021 collected from Baoding, Hebei Province and the adzuki bean variety Baihong 2 with better comprehensive traits in Jilin Province were selected as hybrid parents.

[0029] Wherein, the earlier-maturing rice bean parent material F021 in Baoding, Hebei Province is preserved and provided by the Grain and Oil Crops Research Institute of Hebei Academy of Agriculture and Forestry Sciences.

[0030] Baihong 2 is an adzuki bean variety bred in Jilin Province, which can be applied for or purchased from Jilin Baicheng Academy of Agricultural Sciences.

[0031] 2. Test method

[0032] Material planting: sow the parent materials in flower pots, among which the female parent material Fandou F021 was planted in 5 pots, and 3 seedlings were reserved in each pot. The male parent material Baihong 2 was sown in two batches. The f...

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Abstract

The invention discloses a method for realizing distant hybridization of adzuki beans and rice beans by utilizing young embryo rescue. The method comprises the steps of (1) preparation of materials, namely, selecting more early-matured rice bean material as female parent, selecting adzuki bean variety with excellent comprehensive characters as male parent, and selecting disease-free full grains for planting; (2) material planting; (3) cross pollination; (4) young embryo rescue; (5) hybrid seedling transplanting. According to the method, the more early-matured rice bean material is taken as the female parent, and the adzuki bean variety with excellent comprehensive characters is taken as the male parent, so that the obtained hybrid young embryos can directly grow into seedlings, the seedlings are good in growth and development and high in survival rate of transplanting, distant hybridization F1 plants can normally bloom and bear fruit at the adult-plant stage, and seeds of F2 can be harvested. After the method is used, a great deal of distant hybridization embryos can continue to develop into normal seeds, abortion of distant hybrid embryos can be effectively avoided, a great deal of distant hybridization filial generations can be obtained, and interspecific distant hybridization of the adzuki beans and the rice beans can be finally realized.

Description

technical field [0001] The invention relates to a method for distant hybridization of adzuki bean and rice bean, in particular to a method for realizing distant hybridization of adzuki bean and rice bean by using immature embryo rescue, and the invention belongs to the field of biotechnology breeding. Background technique [0002] Adzuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] is native to China, and is planted all over my country except for a few alpine regions. The main production areas are in North China, Northeast China and the Jianghuai region. my country is the world's largest producer of small soybeans, and its total output and export volume both rank first in the world. Bean weevil is a kind of storage pest in the production process of adzuki bean. The weight of adzuki bean harmed by bean weevil is reduced, the nutrition is lost, and the quality is seriously reduced. The main method for preventing and controlling soybean weeds in production is aluminum phosphi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘长友田静范保杰曹志敏苏秋竹张志肖王彦
Owner INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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