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Method of constructing clone groups of lily hybridization generation

A clone and lily technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the constraints of the scale and industrialization of the lily flower breeding industry, the demand for new varieties of lily that are difficult to self-fertilize, and the start of breeding To solve the late problem, achieve the effect of speeding up lily breeding, good consistency of traits, and simple process

Inactive Publication Date: 2013-02-06
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, almost all the cultivated lily varieties in my country come from the Netherlands. Although the domestic lily breeding industry has made great progress in recent years, the number of lily varieties cultivated is still not comparable to that of foreign powers.
Due to the late start of lily breeding in my country, and the cultivation cycle of lily hybrid offspring bulbs is long, the cycle from hybrid seeds to flowering is as long as 4 to 5 years, and it takes at least 11 years to breed a variety. Such a long breeding cycle Seriously restricting the large-scale and industrialized development of the lily flower breeding industry, it is difficult to meet the market demand for new varieties of self-cultivating lilies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A, the style of the female parent oriental lily 'Sorbonne' is cut off the part connecting the stigma at the upper part with a blade, and the part of 0.5cm above the ovary is reserved, and the fresh pollen of the male parent 'Lanzhou lily' is applied to the cut for pollination, and then used Wrap the cut with tinfoil;

[0020] B. Within 90 days after the pollination in step A, when the fruit development stops and begins to shrink, the lily capsules developed from the ovary are picked;

[0021] C, be that the volume concentration of the lily capsule obtained in step B is 75% alcohol to wipe the fruit surface 3 times, then soak for 1 minute with the volume concentration of 75% alcohol, and then treat it in a mercuric chloride solution of 0.1% in mass concentration for 15 minutes , and finally washed twice with sterile water;

[0022] D. Cut open the lily capsules treated in step C, pick out the seeds with embryos and tear off the seed coat with the tip of tweezers under a...

Embodiment 2

[0027] A. The style of the female Oriental lily 'Casa Blanca' is cut off with a blade to connect the upper part of the stigma, and the part 0.8 cm above the ovary is reserved, and the fresh pollen of the male Asian lily 'Brunello' is applied to the incision for pollination. Cover the incision with tin foil;

[0028] B. Within 57 days after the pollination of step A, when the fruit develops slowly and starts to turn yellow, adopt the lily capsules developed from the ovary;

[0029] C. Wipe the fruit surface with 72% alcohol to wipe the fruit surface 3 times with the lily capsule obtained in step B, then soak it in 72% alcohol for 1 minute, and then treat it in 0.1% mercuric chloride solution for 15 minutes with a mass concentration , and finally washed twice with sterile water;

[0030] D. Cut open the lily capsules treated in step C, pick out the seeds with embryos and tear off the seed coat with the tip of tweezers under a dissecting microscope, scratch lightly on the endosp...

Embodiment 3

[0035] A, the style of the female parent OT lily 'Manissa' is cut off the part connecting the stigma at the upper part with a blade, and the part 1 cm above the ovary is reserved, and the fresh pollen of the male parent Oriental lily 'Bernini' is applied to the cut for pollination, and then used Wrap the cut with tinfoil;

[0036] B, within 30 days after the pollination of step A, when the fruit development is slow, adopt the lily capsule developed by the ovary;

[0037] C. Wipe the fruit surface with 70% alcohol to wipe the fruit surface 3 times with the lily capsule obtained in step B, then soak it in 70% alcohol for 1 minute, and then treat it in 0.1% mercuric chloride solution for 15 minutes , and finally washed twice with sterile water;

[0038] D. Cut open the lily capsules treated in step C, pick out the seeds with embryos and tear off the seed coat with the tip of tweezers under a dissecting microscope, scratch lightly on the endosperm, extrude white translucent immat...

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Abstract

The invention provides a method of constructing clone groups of lily hybridization generation. The method comprises the following steps of cutting stigmas of screened lily target parents, hybridizing and collecting; performing embryo rescue such as germinating, multiplying and heading; expanding propagation from one plant to hundreds of plants; vernalizing after bulblets grow; cultivating the bulblets to soil of a greenhouse after treatment; overwintering twice; digging the bulblets out before the third winter; and thus obtaining hundreds of the individual flowering clone groups. Due to the adoption of the method, the period is greatly shortened from eleven years to five years, so that the breeding speed of lily is increased, and the efficiency of breeding new varieties of lily is improved. The method is simple in process, has obvious effects of obtaining lily distant hybridization generation of the lily and quickly constructing hybrid clone groups, and the traits of all plants in the groups are good consistently.

Description

technical field [0001] The invention relates to a method for constructing a hybrid progeny clone population, in particular to a method for constructing a lily hybrid progeny clone population, and belongs to the technical field of plant breeding. Background technique [0002] In the early 20th century, European and American countries carried out a lot of research on the breeding technology of new lily varieties. By 2008, the number of officially registered lily varieties in the world had reached 9,465. The Netherlands, a European lily breeding powerhouse, can release nearly 200 new lily varieties every year. At present, almost all of my country's cultivated lily varieties come from the Netherlands. Although the domestic lily breeding industry has made great progress in recent years, the number of cultivated lily varieties still cannot be compared with foreign powers. Due to the late start of lily breeding in my country, and the cultivation cycle of lily hybrid progeny bulbs ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
Inventor 崔光芬吴丽芳王祥宁王继华贾文杰段青唐开学马璐琳张艺萍
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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