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Method for improving domestication survival rate of distant-hybridization tissue cultured seedlings of cucumbers

A technology of distant hybridization and tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of waste of rare materials, high survival rate, difficult to achieve, etc., to improve the germination rate, speed up reproduction, The effect of reducing the degree of callusization and vitrification

Inactive Publication Date: 2013-12-11
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0008] In the above-mentioned reports, people mainly conducted research on plant regeneration conditions from the perspective of transgenics, but these systems are not completely suitable for cucumber distant hybrid materials, especially in the stage of transplanting and domestication, and it is difficult to achieve a high survival rate. , resulting in a waste of precious materials

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  • Method for improving domestication survival rate of distant-hybridization tissue cultured seedlings of cucumbers
  • Method for improving domestication survival rate of distant-hybridization tissue cultured seedlings of cucumbers
  • Method for improving domestication survival rate of distant-hybridization tissue cultured seedlings of cucumbers

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Embodiment Construction

[0024] 1. Embryo culture: wash a hybrid fruit with the cultivated cucumber 'Baisitiao' as the female parent and the wild cucumber related wild species C. hystrix as the male parent, and then dip it in 75% ethanol with absorbent cotton After surface disinfection, spread high-temperature sterilized filter paper on the ultra-clean workbench, use tweezers to clamp out the unfulfilled seeds in the fruit, remove the capsule, cut longitudinally to remove 1 / 2 of the testa, and insert the embryo into MS+0.2mg / L6-BA+60mg / L sucrose medium for embryo culture, temperature 25 ℃, light 2500lx. After 6 days of cultivation, the germinated embryos were transferred to fresh MS basic medium, and other conditions remained unchanged, and sterile seedlings could be obtained one week later.

[0025] 2. Induction of clustered adventitious buds: Carefully cut off the terminal buds or axillary buds (about 0.5-1.5 cm) of the above-mentioned sterile seedlings on an ultra-clean workbench covered with ster...

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Abstract

The invention relates to a method for improving the domestication survival rate of distant-hybridization tissue cultured seedlings of cucumbers, belonging to the field of biotechnology. The method is mainly used for propagation and preservation and domestication seedling of hybridization materials of cucumber species and provides technical guarantee for application of a distant hybridization technology to cucumber breeding. The method mainly includes the steps of performing embryo rescue to obtain aseptic seedlings, carrying out induced differentiation on adventitious buds, carrying out regeneration bud extension and rooting culture and domestication transplanting, and regulating and controlling a specially prepared culture medium and external environment conditions. The method is simple in operation, has low requirements on raw materials and laboratory facilities, and has extremely good effects. According to the method, the obtained distant-hybridization tissue cultured seedlings have strong functional roots, the domestication period is shortened, and the survival rate of transplanting is improved. The method can provide a stable and high-efficiency regeneration seedling domestication system for hybridization of two individuals from different cucumber species and hybridization of two individuals from the same cucumis genus, and can be used for preservation and propagation and preservation of treasured cucumis wild species and transplanting seedling of common cucumber tissue cultured seedlings.

Description

technical field [0001] The invention relates to a method for improving the domesticated survival rate of cucumber distant hybrid tissue cultured seedlings, which is particularly suitable for the domesticated seedlings of cucumber interspecies hybrid materials and belongs to the field of biotechnology. Background technique [0002] Cucumber is an important worldwide vegetable, with many germplasm resources, but limited intraspecific variation, which seriously hinders the breeding practice of cucumber. The wild and related germplasm resources of Melon have a variety of disease resistance and stress resistance genes, which are important exogenous gene banks for cucumber variety improvement. Distant hybridization is an important way to transduce and utilize exogenous useful traits. Chen Jinfeng took the lead in successfully obtaining hybrids between cucumber and the wild species C. hystrix through distant hybridization, and then obtained a series of interspecific heterochromati...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈劲枫贾利娄群峰钱春桃李季
Owner NANJING AGRICULTURAL UNIVERSITY
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