Method for getting excised embryo by virtue of intergeneric distant hybridization between radish and kohlrabi
A technology of distant hybridization and kohlrabi, which is applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problem of no distant hybridization between the two, and achieve the effect of improving compatibility.
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[0022] 1 Materials and methods
[0023] 1.1 Test materials
[0024] The radish male sterile line DH303A was used as the female parent (2n=18), and kohlrabi was used as the male parent (2n=18). The experimental materials were commercial products purchased from the Vegetable Science and Technology Research Center of Hubei Academy of Agricultural Sciences.
[0025] 1.2 Test method
[0026] This experiment was carried out at the Vegetable Science and Technology Center of Hubei Academy of Agricultural Sciences.
[0027] 1.2.1 Seeding
[0028] The kohlrabi and radish seeds were sown in the experimental greenhouse, and they were subjected to conventional field management.
[0029] 1.2.2 Bagging and tagging
[0030] The radish and kohlrabi are bagged after bolting. If there are flowers that have opened before bagging, the flowers should be removed. Each individual plant of the male sterile line of radish is bagged separately and marked with a serial number. There are 10 individua...
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