Extraction method of vegetable protein

An extraction method and plant protein technology, which is applied in the field of plant protein extraction, can solve the problems of low protein yield, low protein purity, and damage to the spatial structure, and achieve the goals of reducing NaCl crystals, high protein purity, and high extraction rate Effect

Active Publication Date: 2020-11-27
NINGBO UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, during the extraction process of the plant protein involved in the above-mentioned patent, the alkali in the alkali-dissolving and acid-precipitating method will destroy the structure of the amino acid, and even generate toxic substances, and a large amount of salt residue is introduced in the process, and the purity of the obtained protein is not high. , the yield of protein is not high; the high cost of enzymatic method and the problems of mostly small molecular polypeptides limit its application; high pulse electric field can induce the polarization of protein molecules and destroy its spatial structure, thus affecting its functionality

Method used

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  • Extraction method of vegetable protein
  • Extraction method of vegetable protein
  • Extraction method of vegetable protein

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Effect test

Embodiment 1

[0031] Use a high-speed grinder to crush the hemp seeds, pass through a 40-mesh sieve, use n-hexane as a solvent, add n-hexane at a material-to-liquid ratio of 1:4, mechanically stir for 30 minutes, then centrifuge at 5600xg for 10 minutes, and continue to operate in this way for insoluble matter After degreasing for 3 times, dry it, take 100g of degreased hemp seed powder and add deionized water according to the ratio of material to liquid 1:20, use NaOH solution to adjust the pH to 10.0, stir mechanically for 2 hours, during which the pH of the solution is detected every 15 minutes and ensure Its value is 10.0, after centrifugation at 5600xg for 10 minutes, the supernatant is placed in an electric field, graphite is used as the anode plate, titanium alloy is used as the cathode plate, the power supply voltage is adjusted to 15V, and the distance between the plates is 10cm. After 60 minutes, the power was turned off, and the collected flocs were vacuum freeze-dried at -48°C fo...

Embodiment 2

[0034] Use a high-speed grinder to crush the hemp seeds, pass through a 60-mesh sieve, use petroleum ether as a solvent, add petroleum ether according to the ratio of material to liquid 1:3, mechanically stir for 30 minutes, then centrifuge at 5600xg for 10 minutes, and continue to operate in this way for insoluble matter After degreasing for 3 times, dry it, take 100g of degreased hemp seed powder and add deionized water according to the ratio of material to liquid 1:15, use NaOH solution to adjust the pH to 10.5, stir mechanically for 2 hours, during which the pH of the solution is detected every 15min and ensure Its value is 10.0, after centrifugation at 5600xg for 10 minutes, the supernatant is placed in an electric field, graphene is used as the anode plate, titanium alloy is used as the cathode plate, the power supply voltage is adjusted to 30V, and the plate spacing is 8cm, and the cathode plate and its vicinity are collected every 10 minutes. After 10 minutes, the power...

Embodiment 3

[0036] Use a high-speed pulverizer to crush the quinoa seeds, pass through a 40-mesh sieve, use n-hexane as a solvent, add n-hexane at a material-to-liquid ratio of 1:2, mechanically stir for 30 minutes, then centrifuge at 5600xg for 10 minutes, and continue to operate in this way for insoluble matter After degreasing for 3 times, dry it, take 100g of degreased quinoa powder and add deionized water according to the ratio of material to liquid 1:15, use NaOH solution to adjust the pH to 9.0, stir mechanically for 2 hours, during which the pH of the solution is detected every 15min and ensure its The value is 9.0, then centrifuged at 5600xg for 10 minutes, the supernatant is placed in an electric field, graphite is used as the anode plate, and copper alloy is used as the cathode plate. After 40 minutes, the power was turned off, and the collected flocs were vacuum freeze-dried at -48°C for 12 hours to obtain quinoa seed protein isolate.

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Abstract

The invention discloses an extraction method of vegetable protein. The extraction method is characterized by comprising the following steps: (1) crushing plant raw materials into powder; (2) mixing the powder obtained in the step (1) with an organic solvent, performing uniform stirring and centrifuging, collecting insoluble substances, and then air-drying the insoluble substances to obtain degreased powder; (3) mixing the degreased powder obtained in the step (2) with deionized water, regulating the pH value to 8.5-11.0 by using alkali liquor, then performing centrifuging, and retaining supernatant; and (4) placing the supernatant obtained in the step (3) in an electric field with the electric field intensity of 0.42-3.75V / cm, collecting flocculate on an electrode plate, and finally freeze-drying the flocculate to obtain the required vegetable protein. Compared with the prior art, the preparation method disclosed by the invention can be used for quickly and efficiently obtaining the vegetable protein with high purity and high integrity.

Description

technical field [0001] The invention relates to the field of protein extraction, in particular to a plant protein extraction method. Background technique [0002] Protein is an indispensable and important material basis in life activities, and it is the main form that human beings rely on for survival. At present, the main sources of protein for human beings are animal protein and plant protein. Because the types and proportions of amino acids contained in animal protein are more in line with the needs of the human body, human amino acid intake has been mainly from animal protein for a long time. Western European countries mostly rely on animal-based diets, and traditional animal-based protein foods contain a lot of fat, cholesterol and hormones. Long-term excessive consumption can easily lead to obesity, high blood pressure, high blood fat and various cardiovascular and cerebrovascular diseases. In order to meet people's ever-improving living standards, it has become an ur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/24
CPCC07K1/145C07K1/24
Inventor 王亚娟仇丹杨人元李淳雄李亚姚利辉王灵辉
Owner NINGBO UNIVERSITY OF TECHNOLOGY
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