Nucleic acid sequence for coding human choline acetyl transferase or fusion protein of human choline acetyl transferase and application of nucleic acid sequence

A technology of nucleic acid sequence and coding sequence, which is applied in the field of nucleic acid sequence encoding human choline acetyltransferase or its fusion protein, and preparation of transduction peptide-human choline acetyltransferase fusion protein, which can solve the problem of easy formation of inclusion bodies The expression level and the difficulty in expressing human choline acetyltransferase have achieved the effects of safe clinical medication, high enzyme activity and high protein purity

Active Publication Date: 2018-03-06
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, at present, the expression of human choline acetyltransferase or its fusion protein is difficult, easy to form inclusion bodies or the expression level is extremely low

Method used

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  • Nucleic acid sequence for coding human choline acetyl transferase or fusion protein of human choline acetyl transferase and application of nucleic acid sequence
  • Nucleic acid sequence for coding human choline acetyl transferase or fusion protein of human choline acetyl transferase and application of nucleic acid sequence
  • Nucleic acid sequence for coding human choline acetyl transferase or fusion protein of human choline acetyl transferase and application of nucleic acid sequence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1: Construction of the recombinant expression vector of the transduction peptide-ChAT fusion protein

[0079] According to ChAT nucleic acid sequence (SEQ ID NO:1) and transduction peptide nucleic acid sequence (SEQ ID NO:3) and Linker nucleic acid sequence (SEQ ID NO:5), the whole gene (SEQ ID NO:7) was synthesized, and its two ends It contains Nco I and BamH I restriction sites, and is inserted into the fusion protein expression vector pET15b vector (Novagen Company). The resulting recombinant vector was named pET15b-PTD-ChAT. The gene sequencing analysis was correct. (Shanghai Shengong Biological Engineering Company).

Embodiment 2

[0080] Example 2: Expression and purification of transduction peptide-ChAT fusion protein

[0081] The recombinant expression vector pET15b-PTD-ChAT obtained in Example 1 was transformed into Escherichia coli strain Ecoli.BL21(DE3), and the recombinant strain was obtained by screening, which was named E.coli BL21(DE3) / pET15b-PTD-ChAT. for protein expression and purification. Can be stored frozen.

[0082] After resuscitating and activating the recombinant strains obtained above or freezing the recombinant strains, spread them evenly on the LB solid medium plate, and put them into a constant temperature incubator at 37°C for overnight cultivation. A single colony was picked from the plate and inoculated into LB liquid medium (containing Amp+, 100 μg / ml), and cultured overnight at 37°C and 220 r / min as the primary seed. The first-grade seeds were inoculated into 5 kinds of medium (including Amp+) with a volume fraction of 5%, and continued to culture to a certain cell concen...

Embodiment 3

[0132] Example 3: Optimization of Transduction Peptide-ChAT Formulations

[0133] The prepared transduction peptide-ChAT stock solution was made into freeze-dried powder after adding different concentrations of trehalose, mannitol, PEG4000, Tween 80, sorbitol or other protective agents (sucrose, PVP, BSA, etc.). Place it in a 37-degree incubator for 1 week, detect the activity of the transduction peptide-ChAT with reference to the method in Example 2 above, calculate the relative activity, further design the composition of the formula, and determine the formulation of the transduction peptide-ChAT, Determine the best storage method. Three samples were taken in each group, and the average value was calculated. The experimental setup and results are shown in Table 3 below and attached image 3 .

[0134] Table 3: Effects of different concentrations of protective agents on transduction peptide-ChAT

[0135]

[0136] Wherein, relative activity=(enzyme activity after test / ...

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Abstract

The invention belongs to the field of biological medicines, and relates to a nucleic acid sequence for coding human choline acetyl transferase or fusion protein of the human choline acetyl transferaseand application of the nucleic acid sequence. The invention further relates to a recombinant vector containing the nucleic acid sequence, a recombinant host cell containing the recombinant vector anda method for preparing transduction peptide-human choline acetyl transferase fusion protein. The nucleic acid sequence can efficiently express the human choline acetyl transferase or the fusion protein of the human choline acetyl transferase, is high in protein activity and capable of being applied to preparing medicines for treating myasthenia, improving the anti-fatigue capability or enhancingthe exercise ability.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a nucleic acid sequence encoding human choline acetyltransferase or its fusion protein and its application. The present invention also relates to a recombinant vector containing the nucleic acid sequence, a recombinant host cell containing the recombinant vector and a method for preparing transduction peptide-human choline acetyltransferase fusion protein. Background technique [0002] Choline Acetyltransferase (Choline Acetyltransferase, ChAT, EC 2.3.1.6) was discovered in 1943, which is the biosynthetic enzyme of neurotransmitter Acetylcholine (Acetylcholine, ACh) (Nachmansohn D, MachadoAL. The formation of acetylcholine. A new enzyme: Choline acetylase.JNeurophysiol.1943; 6:397-403), specifically reflects the functional state of cholinergic neurons, and is also a marker enzyme of the cholinergic nervous system. [0003] ACh is the first identified neurotransmitter (Loewi O. Pflugers ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/62A61K38/45A61K47/42A61P21/04A61P21/00
CPCA61K38/00C07K2319/10C12N9/1029C12Y203/01006
Inventor 李前孙曼霁任汝通李梦李国兴赵宝全
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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