Method for establishing seroglycoid N-glycome atlas model of chronic hepatitis liver injury

Abstract

The invention discloses a method for establishing a seroglycoid N-glycome atlas model of chronic hepatitis liver injury. The method comprises the following steps: detecting a seroglycoid N-glycome atlas by using a G-Test detection method, establishing an N-glycome atlas model with significant difference between liver injury patients and normal control personnel, and screening NG1A2F with significant expression difference between a liver injury group and a normal control group. Based on the N-glycome atlas model established by the method disclosed by the invention, the detection sensitivity andspecificity of liver injury respectively reach 80.0% and 74.4%. In subsequent applications, the degree of liver injury of personnel to be detected can be detected by comparing the peak values of single peak NG1A2F in the N-glycome atlas of the serum of the personnel to be detected and in the atlas model established by the method. Based on the method disclosed by the invention, a plurality of patients with chronic hepatitis liver injury can be subjected to routine and non-invasive detection to help doctors and the patients to monitor the occurrence and progression of the chronic hepatitis liver injury in time, and the method is expected to be promoted and used in clinical practice.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a method for establishing a serum glycoprotein N-glycogroup map model of chronic hepatitis liver injury. Background technique [0002] Chronic hepatitis (Chronic Hepatitis) is a general term for chronic liver inflammation. It is caused by a variety of pathogenic factors, such as viruses, chemical poisons, drugs, alcohol, autoimmune factors, etc., which damage the liver cells, resulting in damage to the liver function and causing body inflammation. A series of uncomfortable symptoms, as well as abnormalities in liver function indicators. Chronic hepatitis B (CHB) is caused by prolonged infection with hepatitis B virus (HBV). There are more than 400 million people infected with hepatitis B virus (hepatitis B virus, HBV) in the world, and nearly 200 million people in China are chronically infected with hepatitis B virus. Most infected people are asymptomatic and do not know they ...

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Problems solved by technology

[0007] Aiming at the problems that in the existing detection of chronic hepatitis liver injury, the specificity and accuracy of blood detection and imaging detection are not high, and the pathological examination is invasive, sampling error, and poor patient dependence, the present invention provides a chronic hepatitis liver injury detection method. The establishment method of the serum glycoprotein N-glycan map model, through the establishment of the model, screen out NG1A2F (galactose single deletion core fucose (α1, 6Fuc) two antennae (single agalacto, core-α-1, 6-fucosylatedbiantennary)) as a specific marker, can be used for the diagnosis of chronic hepatitis liver injury

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