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Sulphating grifola frondosa insoluble polysaccharide and application

A technology of water-insoluble polysaccharides and Grifola frondosa polysaccharides, which can be used in allergic diseases, drug combinations, etc., can solve problems such as patent publications that have not yet been found, and achieve the effects of enhancing immunity, convenient post-processing, and increasing production volume

Active Publication Date: 2016-03-16
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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  • Sulphating grifola frondosa insoluble polysaccharide and application
  • Sulphating grifola frondosa insoluble polysaccharide and application
  • Sulphating grifola frondosa insoluble polysaccharide and application

Examples

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Comparison scheme
Effect test

Embodiment 1

[0047] A kind of sulfated Grifola frondosa water-insoluble polysaccharide, the steps of preparation method are as follows:

[0048] ⑴ Using Grifola frondosa polysaccharide as raw material, swell the Grifola frondosa polysaccharide with N,N-dimethylformamide, the ratio mg:mL of the Grifola frondosa polysaccharide to N,N-dimethylformamide is 200- 300: 1-5mL, then add sulfur trioxide trimethylamine salt [(CH 3 ) 3 N·SO 3 ], the mass ratio of the Grifola frondosa polysaccharide to trimethylamine sulfur trioxide is 400-600:1-4, stirred and reacted in a water bath at 50-90°C for 5-7h to obtain a reaction product;

[0049] (2) Cool the reaction product to room temperature, centrifuge at 3000-5000r / min to discard the supernatant, add absolute ethanol to the precipitate, centrifuge at 3000-5000r / min for 8-15min, and wash the polysaccharide 3 times with ethanol until the sulfur trioxide trimethylamine salt is dissolved. The fishy smell is eliminated, and there are many sugar deposits...

Embodiment 2

[0072] A kind of sulfated Grifola frondosa water-insoluble polysaccharide, the steps of preparation method are as follows:

[0073] (1) Using Grifola frondosa polysaccharide as raw material, swell 400-600mg of Grifola frondosa polysaccharide with 2-10mL N,N-dimethylformamide, add 1-4g sulfur trioxide trimethylamine salt, and bathe in water at 50-90°C Stir the reaction for 5-7h;

[0074] (2) Cool the reaction product to room temperature, centrifuge at 3000-5000r / min to discard the supernatant, precipitate and add absolute ethanol, centrifuge at 3000-5000r / min for 8-15min, and wash the polysaccharide 3 times with ethanol until the fishy smell of trimethylamine odor elimination;

[0075] (3) Dissolve the obtained polysaccharide precipitate in 30-70ml distilled water, adjust the pH to 7 with NaOH solution, and centrifuge at 4000r / min for 20min;

[0076](4) Pack the supernatant obtained after centrifugation into a dialysis bag, run water dialysis for 1-3d, distilled water dialysi...

Embodiment 3

[0089] A kind of sulfated Grifola frondosa water-insoluble polysaccharide, the steps of preparation method are as follows:

[0090] Swell 500mg of GFP (Grifola frondosa polysaccharide) with 6mL of DMF, add 2.5g of trimethylamine sulfur trioxide [(CH3)3N·SO3], and stir in a water bath at 80°C for 6h. Cool the reaction product to room temperature, discard the supernatant by centrifugation, add absolute ethanol to the precipitate, centrifuge at 4000r / min for 10min, and wash the polysaccharide with ethanol centrifugation repeatedly for 3 times until the fishy smell of trimethylamine is eliminated. The obtained polysaccharide precipitate was dissolved in 50 ml of distilled water, adjusted to pH 7 with NaOH solution, and centrifuged at 4000 r / min for 20 min. Put the supernatant obtained after centrifugation into a dialysis bag (COMW7000), dialyze with running water for 2 days, and dialyze with distilled water for 1 day. Add ethanol to the dialysate in the bag, and the ethanol conten...

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Abstract

The invention relates to sulphating grifola frondosa insoluble polysaccharide. A preparation method comprises the steps of taking grifola frondosa polysaccharide as a raw material, using N,N-dimethylformamide for swelling, adding sulfur trioxide trimethylamine, and obtaining sulphating grifola frondosa insoluble polysaccharide through water bath reaction, cooling, centrifugation, washing and dialysis. When the polysaccharide is at a certain concentration and time range, an S-GFP can remarkably improve proliferation and phagocytic activity of RAW264.7 cells and have dosage dependence, the optimal action time is 36 h, the optimal action dosage is 40 [mu]g / mL, and secretion and activity of cell acid phosphatase, lysozyme and superoxide dismutase are improved remarkably. The expression of RAW264.7 cell iNOS genes is induced and up-regulated at the mRNA transcriptional level, the generation amount of cell NO is increased, the secretion of cytokines such as IL-2, IL-12, IFN-gamma and IL-6 is promoted, and the sulphating grifola frondosa insoluble polysaccharide is high in immune enhancement activities and can be applied to preparation of medicines or health care products for enhancing immunity.

Description

technical field [0001] The invention belongs to the technical field of food, and relates to a polysaccharide and a preparation method thereof, in particular to a sulfated Grifola frondosa water-insoluble polysaccharide and its application. The sulfated Grifola frondosa water-insoluble polysaccharide can enhance immunity. Background technique [0002] Grifola frondosa (Grifola frondosa), also known as polypore bacterium, thousand Buddha bacteria, etc., is native to northeastern Japan, North America and other places, and is a precious medicinal and edible fungus. Due to the simple breeding method and large yield of Grifola frondosa, it is widely distributed around the world. Among the most important fungi used in the world, the yield of Grifola frondosa ranks second. [0003] Grifola frondosa extract D-fraction has obvious anti-tumor function. This achievement was first reported in the International Medicinal Chemistry Journal "Chemistry and Drug Bulletin" in 1987; The study ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/04
Inventor 程代王春玲侯丽华陈媛媛韩丽荣孟梦
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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