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Gene capable of improving quality of plant fruit amylopectin, encoding product and application of gene

A fruit and gene technology, which is applied to the gene for improving the quality of plant fruit amylopectin and its encoded product and application field, can solve problems such as no related reports, and achieves increased expression, improved starch quality, and improved fruit quality and quality. Effect

Active Publication Date: 2017-01-18
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although SS has been studied in many plants, there are no related reports on fresh starch-converted fruits, such as banana, kiwi, mango, etc.

Method used

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  • Gene capable of improving quality of plant fruit amylopectin, encoding product and application of gene
  • Gene capable of improving quality of plant fruit amylopectin, encoding product and application of gene
  • Gene capable of improving quality of plant fruit amylopectin, encoding product and application of gene

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Embodiment Construction

[0018] The present invention will be further described below in conjunction with specific examples, so as to better understand the present invention.

[0019] 1. Gene acquisition

[0020] Using the cDNA of banana fruit as a template, the

[0021] 5'-CCCATGGGATGTTCCGTGTTTCA-3'

[0022] 5'-GACTAGTCTTATGATACGTGCCTG-3'

[0023] As a primer, a base sequence obtained by PCR amplification is 2397bp ( figure 1 ), its sequence is shown in SEQ ID No.1; the amino acid sequence of the gene encoding MaSSIII-1 is shown in SEQ ID No.2.

[0024] 2. Construction of expression vector

[0025] The nucleotide sequence of the above-mentioned banana fruit soluble starch synthase gene MaSSIII-1 was double-digested with Nco I and Spe I two restriction endonucleases respectively to the target fragment and the pCAMBIA-1302 vector plasmid, and the digested The target fragment and the plant expression vector pCAMBIA-1302 fragment were recovered, connected, transformed and sequenced to verify that th...

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Abstract

The invention relates to a banana fruit soluble starch synthetase gene MaSSIII-1 which has the nucleotide sequence as shown in SEQ ID NO:1, and protein which is encoded by the banana fruit soluble starch synthetase gene MaSSIII-1 has the nucleotide sequence as shown in SEQ ID NO:2. The invention also relates to application of the banana fruit soluble starch synthetase gene MaSSIII-1. The gene MaSSIII-1 provided by the invention can improve the quality of fruit amylopectin and improve the quality of the fruit. For example, the gene is introduced into a tomato, two independent strains of 35S promoter driven MaSSIII-1 transgenic tomatoes are obtained, the shapes of starch granules (obvious cross cracks occur on the surfaces of the starch granules) are changed through overexpression of the MaSSIII-1A, the expression quantity of the MaSSIII-1 is increased, the enzymatic activity of SS (soluble starch synthetase) is improved and the amylopectin content is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to a gene and its application, and also relates to the protein encoded by the gene, in particular to a gene for improving the quality of plant fruit amylopectin, its encoded product and its application. Background technique [0002] The starch metabolism of banana fruit directly affects the yield and quality of bananas in my country and the world. The study of its key enzyme genes is the basis for increasing yield, improving quality, and meeting the diversified needs of starch quality. Soluble starch synthase (SS) is mainly involved in the metabolism of amylopectin. It adds glucose residues in ADPG to the non-reducing end of the side chain through an α-1,4-D-glycosidic bond, and its activity is significantly positively correlated with the accumulation of amylopectin. So far, four SS isoenzymes have been reported, namely SSI, SSII, SSIII, and SSIV (Ball and Morell, 2003). SSI extends the shor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/10C12N15/82C12N15/11A01H5/00
CPCC12N9/1051C12N15/8245C12Y204/01021
Inventor 苗红霞孙佩光徐碧玉金志强张凯星刘菊华张建斌贾彩虹王静毅王卓
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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