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Method for regulating starch contents of rice grains based on ZmMIKC2a gene

A starch content, rice grain technology, applied in the field of genetic engineering, can solve the problems of nutritional and health status effects, increased production costs, etc.

Active Publication Date: 2017-10-13
INST OF TOBACCO ANHUI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This meeting greatly increases the production cost
In addition, human nutrition and health are seriously affected by the consumption of unstructured starches

Method used

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  • Method for regulating starch contents of rice grains based on ZmMIKC2a gene
  • Method for regulating starch contents of rice grains based on ZmMIKC2a gene
  • Method for regulating starch contents of rice grains based on ZmMIKC2a gene

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Embodiment 1

[0024] 1. Materials

[0025] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0026] 2. Method

[0027] 2.1 Acquisition of ZmMIKC2a gene

[0028] Grain endosperm of corn B73 variety 16 days after pollination was selected, corn endosperm RNA was extracted and reverse-transcribed into cDNA, and corn endosperm cDNA was used as a template, based on the ZmMIKC2a gene sequence and the corn B73 genome database, combined with the design of multiple cloning sites for plant expression vectors Primers MIKC2a-F-1 and MIKC2a-R-1 were used for PCR amplification to obtain PCR amplification products.

[0029] The primer sequences are:

[0030] MIKC2a–F-1:5′-GG GGTACC ATGGGTAGGGGAAGGATTGA-3′

[0031] MIKC2a–R-1:5′-TCC CCCGGG TTAGAACTGATGATGAGGGTTAT-3′

[0032] The PCR reaction program was: 98°C, pre-denat...

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Abstract

The invention discloses a method for regulating starch contents of rice grains based on a ZmMIKC2a gene. The ZmMIKC2a gene is a DNA molecule, of which the nucleotide sequence is shown in SEQ ID NO.1. The method comprises the following steps: building an excessive expression carrier of the ZmMIKC2a gene, and importing the excessive expression carrier into a target paddy rice genome to obtain an excessive expression plant of the ZmMIKC2a gene of which straight-chain starch content and the proportion of the straight-chain starch to total starch in seed grains are both higher than that of the target paddy rice. According to the method, positive regulation effects of the ZmMIKC2a gene on a plant seed grain starch synthesis related gene is used, by adopting a genetic engineering method, the straight-chain starch content and the proportion of the straight-chain starch to the total starch in the paddy rice seed grains are changed, so that a new variety of genetically modified paddy rice with high straight-chain starch is obtained, and important practical meaning on development of the new variety of genetically modified paddy rice with a specific function by utilizing a genetic engineering technology is achieved.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for regulating rice grain starch content based on the ZmMIKC2a gene. Background technique [0002] Starch is the main component of plant seeds, and as the end product of photosynthesis, it is an inexhaustible natural material. Starch can be used not only as food and feed, but also as an important industrial raw material, widely used in food, chemical, energy, machinery, construction, pharmaceutical and other industries. In rice, starch is the main component of rice endosperm, and its content and quality directly affect the quality of rice seeds. [0003] Starch is divided into amylose and amylopectin according to different structures. The ratio of amylose and amylopectin in starch and the branch chain structure of amylopectin determine the quality of rice. At the same time, the relationship between amylose and amylose The ratio also determines the purpose o...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/10
CPCC07K14/415C12N15/8245
Inventor 董庆李廷春王芳王俊张玮陈洪俭李成
Owner INST OF TOBACCO ANHUI ACADEMY OF AGRI SCI
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