64 results about "Starch degradation" patented technology

The invention relates to a compound microbial agent which comprises compound bacteria for decomposing kitchen wastes and auxiliary materials for solid fermentation, wherein the compound bacteria comprise 10-15 parts of cellulose degradation bacteria, 15-30 parts of starch degradation bacteria, 15-20 parts of protein degradation bacteria, 15-20 parts of grease degradation bacteria, 10-15 parts of bacillus subtilis and 5-15 parts of pseudomonas aeruginosa; the auxiliary materials comprise 50-60 kg of wheat bran, 15-20 kg of bean pulp, 0.4-0.6 kg of magnesium sulfate, 0.4-0.6 kg of potassium dihydrogen phosphate, 0.1-0.2 kg of disodium hydrogen phosphate and 8-10 kg of glucose. The inoculation amount of the compound bacteria in the auxiliary materials is 0.8%-1%, and the compound bacteria is subjected to solid fermentation to obtain the compound microbial agent. The compound microbial agent has a good decomposing effect on greases, proteins and fiber substances and can be tolerant to salt with a certain concentration; optimally, the degradation rate can reach more than 90%.

Methods and compositions for increasing the starch content in green tissues of a plant are provided. The method comprises down-regulating me activity of starch degradation enzymes in a plant. The resulting transgenic plants of the invention have increased starch content in green tissues and exhibit a starch excess phenotype. In one embodiment the method involves manipulating a monocot plant to down-regulate the activity of a starch degradation enzyme. The plants are useful for improving the yield of free sugars from plant biomass and increase dried green tissue storage stability.

An additive of flue-cured tobacco is prepared from the protein degradation enzyme, starch degradation enzyme, pectin degradation enzyme, poly-phenol oxidase, cellulose, VC and potassium tartrate through adding them to water, stirring for dissolving and filtering to obtain the liquid product. It can be sprayed onto the leaves of tobacco to decreasing the content of organic high-molecular substances in tobacco leaf and improving its quality.

The invention relates to a novel process for producing distilled spirit by pretreating distilled spirit raw and auxiliary materials by means of an extrusion technology and carrying out liquid-state method fermentation. The raw and auxiliary materials for distilled spirit production are pretreated by using the extrusion technology, and reasonable parameters of a distilled spirit raw material extrusion technology and reasonable parameters for liquid-state method distilled spirit production by using the raw materials puffed by extrusion are provided. According to the distilled spirit raw materials pretreated by using the extrusion technology, the compounding effect among materials is better, starch degradation and albuminous degeneration can be performed more thoroughly, so that the utilization rate of the raw materials is increased, and the distilled spirit fermentation cycle is shortened compared with that of the traditional process; and in addition, the flavor, the liquor yield and other multiple qualities of the finished-product distilled spirit are greatly promoted. According to the novel method for brewing by pretreating the distilled spirit raw materials by extrusion, the energy consumption can be saved, the cost is reduced, the raw material utilization rate and the distilled spirit yield are increased, and the fermentation cycle is shortened; and meanwhile, due to multiaspect comprehensive effects, such as sufficient heat effect, mechanical effect and chemical structure modification effect, the yield and quality of the finished-product distilled spirit are greatly promoted.

The invention belongs to the technical field of microorganisms and particularly relates to a bacillussubtilis strain xp producing amylase. A classification designation of the bacillussubtilis strain xp is Bacillussubtilis. The collection number of the strain in China Center for Type Culture Collection (CCTCC) is CCTCC NO: M2011452. The invention further discloses a bacterium-enzyme combined preparation prepared by the strain xp, and an application of the bacterium-enzyme combined preparation in accelerating starch degradation in a tobacco sheet.

A biological additives to expand tobacco and its producing method and applications are disclosed whose compositions and contents are tobacco leaf protein degradation enzyme of 0.1-10%, starch degradation enzyme of 1.0-10%, pectin degradation enuzyme of 0.2-10%, polyose degradation enzyme of 0.5-20%, fiber modifier of 0.5-20%, polyphenol oxidation degradution enzyme of 0.1-5%, carbon chain enzyme of 0.1-10%, vitamin C of 0.1-5% and water of 100%. The invented additives can be added into tabocco after the tabocco is remoistened and cut into pieces but before the tabocco is expanded with adding quantity as 0.2-5% of tobacco pieces weight at the temperature of 30 degree c to 50 degree C. The present invention can not bring negative influence to tobacco but can decrease the composition contents which is harmuful in smoking.

The invention relates to the field of gene engineering, in particular to acid amylase AMYA4 and gene and application thereof. The amino acid sequence of the acid amylase AMYA4 is expressed as SEQ ID NO.1. The optimal pH value of the AmyA4 is 4.2, the optimal temperature is 75 DEG C, and the AmyA4 has high activity at the temperature of between 55 and 75 DEG C and has strong raw starch degrading capability at the same time. The zymology properties meet the process requirement for preparing sugar by a double-enzyme method, so the acid amylase has great application potential. In the process for preparing sugar by simulating the double-enzyme method, no matter paste starch or raw starch is used as a substrate, the starch can reach good hydrolysis rate under the action of matching commercial saccharifying enzyme, so the AmyA4 has huge application potential.

The invention discloses alpha-amylase. A gene sequence of the amylase is shown in SEQ ID NO:2; an amino acid sequence encoded by the gene sequence is shown in SEQ ID NO:1; the gene sequence SEQ ID NO:2 can successfully express the alpha-amylase in an inclusion body form in escherichia coli. By adopting the alpha-amylase obtained by the inclusion body renaturation operation disclosed by the invention, the protein purity can be up to over 85%, and the renaturation rate can be up to over 50%. The alpha-amylase disclosed by the invention can be applied to rapid degradation of raw starch, and is especially applicable to degradation of rice raw starch.

Methods and compositions for increasing the starch content in cob tissues of a plant are provided. The method comprises down-regulating the activity of starch degradation enzymes in a plant. The resulting transgenic plants of the invention have increased starch content in cob tissues. In one embodiment the method involves manipulating a monocot plant to down-regulate the activity of a starch degradation enzyme in cob tissues. The plants are useful for improving the yield of free sugars from plant biomass.

This invention discloses processing method, processing apparatus and saccharification method for beer adjunct added with enzyme preparation by squeezing and cooking. This invention provides squeezing and cooking apparatus for adding enzyme preparation to beer adjunct, reasonable parameters of squeezing and cooking system for adding enzyme preparation to beer adjunct, and saccharification method for beer adjunct added with enzyme preparation. During the saccharification process, the mash of beer adjunct added with enzyme preparation has such advantages as adequate starch degradation, short saccharification time, and high extract yield. Compared with the present double-mash saccharification method and enzyme addition saccharification method, the method in this invention omits beer adjunct cooking and pasting process and apparatus, and adds malt powder and beer adjunct powder or mince added with enzyme preparation into the water in the saccharification pot simultaneously for saccharification, which can save energy, lower cost, and increase beer productivity.

The invention discloses a composite microbial agent and a preparation method and application thereof. The composite microbial agent is prepared by uniformly mixing a cellulose decomposition strain, alignin decomposition strain, protein and a starch degradation strain, wherein the cellulose decomposition strain is one or more of ginseng bacillus, bacillus megatherium and bacillus aryabhattai, thelignin decomposition strain is Klebsiella, and the protein and starch degradation strains are one or more of bacillus thuringiensis, lysine borate bacillus, bacillus licheniformis and bacillus acidophilus. According to the invention, the microbial agent is selected to prevent and treat greenhouse vegetable continuous cropping obstacles, and related functional microorganisms are utilized to condition the nutritional status and microbial structure of the continuous cropping soil, so that the stress resistance of crops is improved, and the purpose of eliminating the continuous cropping obstaclescan be achieved.

The invention discloses a thermophilic acidic raw starch alpha-amylase mutant capable of improving the cornstarch degradation capability as well as a preparation method and application thereof and belongs to the field of genetic engineering and enzyme engineering. According to the thermophilic acidic raw starch alpha-amylase mutant, an SBD structure domain of raw starch alpha-amylase GTamy is subjected to cyclic rearrangement mutation; then SBD in the GTamy is replaced with an SBD cyclic rearrangement mutant, so as to construct a GTamy cyclic rearrangement mutant. The enzyme activity of the GTamy cyclic rearrangement mutant to the cornstarch is compared to screen a raw starch alpha-amylase mutant GTamy-S498 with the cornstarch degradation capability which is remarkably improved. The enzymeactivity of the raw starch alpha-amylase mutant GTamy-S498 provided by the invention on the cornstarch is improved to 114.77 U/mg from 21.08 U/mg of a control group (before mutation) and is improvedby 5.44 times. The cornstarch degradation capability of the raw starch alpha-amylase mutant GTamy-S498 is remarkably improved and the enzymatic property meets the requirements of a starch liquefactiontechnology and is more suitable for the starch liquefaction technology.

The invention discloses a starch-based carbon fiber and a preparation method thereof. The method comprises sequentially performing starch degradation, isomerization, dehydration, cross-linking polymerization, spinning, first pre-oxidation, second pre-oxidation and carbonization, not only Minimize the oxygen-containing groups in the starch molecules, and use the step-by-step pre-oxidation method to reduce the damage of the gas to the fiber structure and reduce the porosity in the fiber, so as to ensure that the prepared starch-based carbon fiber has tensile strength and good tensile modulus.