16 results about "Starch degradation" patented technology

The banana transcription factor MabZIP2 disclosed by the invention is combined with a banana fruit MaLSF1 gene promoter to regulate up-regulated expression of the banana fruit MaLSF1 gene. The MabZIP2 expression is instantly silenced, the expression quantity of the MaLSF1 is reduced, the degradation of banana pulp starch is inhibited, and the content of total starch, amylose and amylopectin is increased, so that the sweet taste of banana fruits is favorably improved, and the quality of bananas is improved; the hardness of the banana pulp is improved, the softening of the banana after picking can be delayed, the ripening of the banana fruit after picking is delayed, and the storage period of the banana fruit is prolonged. The banana transcription factor MabZIP2 provides an important gene resource for improvement of banana fruit quality, provides a theoretical basis for research and development of a banana fresh-keeping technology and cultivation of new storage-resistant varieties, and also provides a new way for research and development of a fresh-keeping technology of starch storage type fruits such as bananas, molecular design breeding and cultivation of new storage-resistant banana varieties.

The invention belongs to the field of biomass-based functional materials, relates to a difunctional coating based on enzymolysis modified cassava starch as well as a preparation method and application of the difunctional coating, and innovatively solves the technical problem that a single coating gives consideration to oil prevention and water absorption through a synergistic technical path of pretreatment and composite enzymolysis. The core mechanism is as follows: the starch particle structure is destroyed through pretreatment, and the degradation degree of starch is accurately controlled through the synergistic effect of alpha-amylase and saccharifying enzyme, so that an unexpected technical effect is achieved, and an enzymolysis product with a film-forming skeleton (medium molecular weight dextrin) and a hydrophilic unit (small molecular saccharide) is formed; meanwhile, a compact and continuous network structure can be formed by a film-forming framework, and grease permeation is blocked; and the hydrophilic unit endows the coating with a rapid capillary water absorption effect.

A method for preparing a roasted food product, suitable for preparing a coffee-like 5 beverage product, starting from one or more pulses, comprising one or more pretreatment steps followed by one or more roasting steps, wherein preferably the roasting temperature is in the range of 75 °C to 250 °C, more preferably in the range of 125 °C to 225 °C, even more preferably in the range of 150 °C to 200 °C, wherein the pretreatment comprises the following steps: ▪ at least one polysaccharide degradation step, preferably for starch degradation; ▪ at least one acidification step, preferably by fermentation, to obtain a pH preferably below 6 of the roasted food product, more preferably below 5.5, most preferably below 5; ▪ at least one drying step, wherein the water content of the pulses obtained by the polysaccharide degradation and acidification steps is adjusted to preferably 14 weight% or less, more preferably to 12 weight%, most preferably to 10 weight% as compared to the total weight of the pretreated pulses.

The application belongs to the field of biology and particularly relates to a low-temperature response transcription factor and application thereof in regulating starch degradation of annona squamosa fruits. The application provides a transcription factor AaHATL which is cloned from annona squamosa for the first time. Research shows that the transcription factor gene is located in the nucleus and is induced by low temperature in transcription and protein level. Overexpression of the gene in tomato can significantly increase the starch content of fruits, which is beneficial to increase the soluble solid content of mature tomato fruits and improve the quality of tomatoes. Transient expression of the gene in annona squamosa can significantly increase the starch content in annona squamosa fruits, delay the postharvest softening of annona squamosa, and prolong the shelf life of annona squamosa. The application provides a new candidate gene for the improvement of tomatoes and annona squamosa and the research of prolonging the shelf life and shelf period of starch storage type fruits.

The application discloses a Paenibacillus sp. NGMCC 1.200843 derived from rhinoceros feces, belongs to a new discovery in the technical field of microorganisms, and is identified as a new species of Paenibacillus, numbered as NGMCC 1.200843=CGMCC 1.64763T, and has been registered and preserved in the China General Microbiological Culture Collection Center. The strain can form a larger transparent circle on a specific culture medium, indicating that the strain has strong phosphorus and starch degradation capacity. Under the current situation of soil fertility decline and fertilizer abuse, the strain provides a high-quality strain resource for the development of microbial fertilizers with organic phosphorus degradation function, and helps to promote the development of microbial fertilizers. The hydrolytic enzymes produced by the strain have wide application potential in the industrial fields of food, fermentation and pharmaceuticals. In particular, amylase can hydrolyze starch to produce various products, and has important significance for the biotechnology industry, and the application range covers multiple industries such as food, fermentation, textile and papermaking. In a word, the application provides a new resource for the development of microbial fertilizers and industrial enzyme preparations.

The invention belongs to the field of biology, and particularly relates to a low-temperature response transcription factor and application of the low-temperature response transcription factor to regulation and control of degradation of annona squamosa fruit starch. The transcription factor AaHATL is cloned from annona squamosa for the first time, and research shows that the transcription factor gene is positioned in a cell nucleus and is induced at low temperature in transcription and protein levels. Overexpression of the gene in tomatoes can significantly improve the starch content of fruits, facilitates improvement of the soluble solid content in mature tomato fruits, and facilitates improvement of the quality of tomatoes. The gene is transiently expressed in annona squamosa, so that the starch content in annona squamosa fruits can be remarkably increased, the after-ripening softening of annona squamosa is delayed, and the storage life of annona squamosa is The invention provides a new candidate gene for research on improvement of varieties such as tomato and annona squamosa, delay of shelf life of starch storage type fruits and the like.

The present invention relates to a method for obtaining a plant-based food ingredient for a dairy alternative food product, where plant material is hydrolyzed at a temperature between 25°-60° C. in the presence of a raw starch degrading alpha-amylase and optionally additional enzymes to obtain hydrolyzed plant material for use as a plant-based food ingredient for a dairy alternative food product.

This invention discloses a dual-layer gradient degradation type fully biodegradable mulch film and its preparation method. The mulch film includes a functional slow-release layer and an induced degradation layer, with a total thickness of 20-50 μm. The functional slow-release layer contains functional nutrient starch, which is based on starch phosphate and compounded with oxalamide, potassium humate, and potassium sulfate. The nutrient release rate is coupled with the starch degradation rate, achieving a slow and controllable release of nitrogen, phosphorus, potassium, and organic matter. The induced degradation layer contains nano-biochar, which can actively enrich soil microorganisms and induce degradation. The preparation method uses a dual-layer co-extrusion blown film process, with the extrusion temperature zones of the two layers set independently and integrally formed. This invention solves the problems of poor controllability of nutrient burst release and degradation initiation in existing mulch films, as well as their large thickness and high cost.

The present application relates to the technical field of strengthening retention aid, and discloses a preparation method of high-efficiency strengthening retention aid containing nanocellulose and application thereof in papermaking, which comprises the following steps: step one: nanocellulose raw materials are added into water for pretreatment, then a modification reagent is added for first modification treatment, so that a modified nanocellulose solution is obtained; step two: starch raw materials and a starch degradation reagent are added into the modified nanocellulose solution for second modification treatment, so that a modified nanocellulose starch solution is obtained; step three: a cationic reagent and a catalyst are added into the modified nanocellulose starch solution for third modification treatment, so that a cationic modification solution is obtained; and step four: a neutralizing reagent, a strengthening agent and a bactericide are added into the cationic modification solution, so that the strengthening retention aid is obtained. Through cationization of nanocellulose and starch, the present application effectively improves the reaction efficiency of cations, and obtains a more stable liquid product, so that the application effect is improved.

The invention discloses bacillus toonensis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is February 2, 2024, and the preservation number is CGMCC No.29852. The bacillus toonensis belongs to the eubacterium field, the posterior mycophylum, the bacilli, the bacillus family and the bacillus genus. The bacterial suspension of the bacillus orientalis can be used for degrading cellulose, starch and straw.

The invention discloses a high-level starch synthesis microorganism, relates to genetic modification for improving the starch synthesis capacity of the microorganism and a genetic engineering bacterium for efficiently synthesizing starch, and belongs to the technical field of biotechnology and genetic engineering. The method comprises the following steps: by taking microorganisms into which exogenous glucose-1-phosphoadenosine transferase and starch synthetase genes are introduced as initial strains, optimizing a starch synthesis route, strengthening starch synthesis precursor supply, weakening a starch degradation process, finely blending a carbon metabolic flow and transforming the form and cell size of the strains; the starch synthesis and accumulation capability of the strain is improved. The genetic modification disclosed by the invention can greatly improve the yield of starch synthesized by microbial cells, the carbon source conversion rate and the production intensity, produces the genetically engineered bacterium which is high in industrial attribute and is capable of efficiently synthesizing starch, and can be applied to industrial biological manufacturing of starch.

A method for starch degradation includes the following steps. Acetylated adipic acid distarch, acid and polar solvent are mixed to form a starch suspension. The temperature of the starch suspension is adjusted to -23℃ to 47℃, and a degradation reaction is performed to form a first degraded starch solution. A base is added to raise the pH value of the first degraded starch solution to obtain a second degraded starch solution. The method for starch degradation has the advantage of simple process. A method for making an oil-proof composite layer includes the following steps. Acetylated adipic acid distarch with a weight average molecular weight of 3.00×10 6 to 5.50×10 7 and a polar solvent are mixed to obtain a starch solution. The starch solution is gelatinized to form a gelatinized starch solution. The gelatinized starch solution is attached to a substrate and a drying process is performed to form an oil-proof composite layer. The oil-proof composite layer has good oil-proof degree and can be applied to various packaging containers that require oil-proof isolation, especially food packaging containers, which has great industrial applicability.

The invention belongs to the technical field of biology, and particularly relates to application of a MaLSF1 gene in regulation and control of banana starch degradation and improvement of fruit quality. According to the banana fruit MaLSF1 gene provided by the invention, the content of total starch and amylopectin in banana fruits overexpressed with the MaLSF1 gene is remarkably reduced, and the change of the amylose content is not influenced. Expression of the MaLSF1 gene is inhibited in banana fruits and fruit slices, the content of total starch and amylopectin is remarkably increased, banana peel presents a stay green phenotype, and the storage period is obviously prolonged by about 2.5 days. The invention provides an important gene resource for improving the flavor quality and appearance quality of bananas, provides a theoretical basis for research and development of a banana fresh-keeping technology and cultivation of new storage-resistant varieties, and also provides a target gene for research and development of a fresh-keeping technology of fruits such as bananas and cultivation of new storage-resistant varieties of bananas.

The invention belongs to the technical field of microorganisms, and discloses an Aspergillus niger strain for efficiently degrading corn starch and cassava starch, a method and application thereof, the strain is constructed by taking Aspergillus niger LH-1 as a starting strain, overexpressing a transcription factor amyR, and overexpressing an amyA gene on the basis to obtain an Aspergillus niger strain CB04; the nucleotide sequence of amyR is shown as SEQ ID NO. 4, and the nucleotide sequence of amyA is shown as SEQ ID NO. 1. The strain disclosed by the invention can efficiently and directly degrade and utilize starch, and not only can reduce the fermentation cost, but also can expand the application range of aspergillus niger in biological manufacturing. The constructed strain can directly take starch as a carbon source to efficiently synthesize citric acid and other compounds, so that the sustainability and economical efficiency of industrial production are improved, the starch degradation efficiency of the constructed strain is improved by 120% or above compared with that of an original strain, and starch in a culture medium can be completely degraded within 5 days.

The invention provides a kelp green rot pathogenic bacterium, the kelp green rot pathogenic bacterium is pseudoalteromonas HKCH18 (pseudoalteromonas sp.), the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is September 4, 2023, and the preservation number is CGMCC NO.28348. The invention further provides a preparation method of the kelp green rot pathogenic bacterium. According to the invention, a strain of pseudoalteromonas HKCH18 is successfully screened, and the pseudoalteromonas HKCH18 has good degradation activity on sodium alginate, agar, sodium carboxymethyl cellulose and starch. When a healthy kelp infection experiment is carried out, healthy kelp can generate green rot diseases, and the green rot diseases are pathogenic bacteria of kelp green rot diseases. The strain can be used for screening kelp strains capable of resisting the kelp green rot disease, and the pathogenesis of the kelp green rot disease can be deeply studied.

Provided is a technology capable of modifying cooked rice cooked using defective rice.　The present technology provides a modifier for defective rice cooking, the modifier comprising a starch degrading enzyme and 4-α-glucanotransferase. The present technology also provides: a method for producing cooked rice, the method involving a rice cooking step for cooking defective rice to which a starch degrading enzyme and 4-α-glucanotransferase have been added; and a method for modifying cooked rice. The present technology further provides cooked rice containing defective rice, wherein the modifier for defective rice cooking according to the present technology is used.