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Preparation method of tubular starch derivative

A starch derivative and starch technology, which is applied in the field of modified starch processing, can solve the problems of low efficiency of debranching enzymes in debranching starch, wide range of tubular starch derivative polymers, and reduced yield of tubular starch derivatives, etc., so as to improve the yield. , The effect of strong embedding ability and narrow distribution range

Active Publication Date: 2013-12-11
JIANGNAN UNIV
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  • Application Information

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Problems solved by technology

However, there are two problems with this method: Amylopectin has a cluster structure, which leads to low debranching efficiency of debranching enzyme, so that the yield of tubular starch derivatives is low; Debranched products have a wide range of polymerization degrees, which is not conducive to the industrial application of tubular starch derivative products
[0003] For the first problem, the existing technology in the starch industry is to use α-amylase to decluster starch, but α-amylase usually hydrolyzes starch into oligosaccharides, which leads to a decrease in the yield of tubular starch derivatives; Solution to wide range of tubular starch derivative polymers

Method used

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  • Preparation method of tubular starch derivative
  • Preparation method of tubular starch derivative

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Embodiment 1

[0021] Disperse 5g of glutinous rice starch in 100mL of distilled water, stir, put in a boiling water bath until the starch is completely gelatinized, and cool to room temperature; in the JY92-2D ultrasonic cell breaker, immerse the ultrasonic probe in the starch paste, and at an ultrasonic frequency of 20kHz, ultrasonic The power is 200W, and the ultrasonic treatment time is 15min in the way of ultrasonication for 3s and pause for 3s; cool to 37°C, adjust the pH to 3.5 with 0.1mol / L acetic acid-sodium acetate buffer solution, add 5mL isoamylase (enzyme activity 5U / mL ) for 12 hours, heated in a boiling water bath for 5 minutes to stop the reaction, and cooled to room temperature.

[0022] Take 10mL of starch debranching solution, under stirring at a rate of 50rpm, add 10mL of ethanol at a rate of 0.16mL / min through a peristaltic pump to make the volume ratio of aqueous solution to ethanol 1:1, continue to stir for 10min, stand still at 4°C for 12h, and Centrifuge at 10,000×g ...

Embodiment 2

[0024] The starch derivatives prepared in Example 1 were dissolved in distilled water and analyzed by high performance anion chromatography. Analysis conditions: Dionex ICS-500 chromatographic system is adopted, the column model is DionexCarboPAC PA200 (250*4mm I.D.); the eluent is: 100mM sodium hydroxide solution (solution A), 100mM sodium hydroxide solution containing 600mM sodium acetate (solution B); Gradient elution: 20% eluent B at 0 min, 100% eluent B at 60 min; the flow rate is 1 mL / min, the injection volume is 25 μL, see figure 2 , figure 2 It shows that the degree of polymerization of the obtained tubular starch derivative is 20-32.

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Abstract

The invention discloses a preparation method of tubular starch derivative, and belongs to the technical field of modified starch processing. The invention relates to tubular starch derivative, which has a narrow polymerization degree range and is prepared through combination of ultrasonic de-cluster starch, isoamylase debranching, and alcohol step precipitation fractionation technologies. The method comprises the main steps that waxy rice starch is selected as a raw material, and then tubular starch derivative products, with the polymerization degree (DP) being 20 to 32, are obtained through gelatinization, ultrasonic de-cluster (ultrasonic processing is performed for 3s, and then ultrasonic processing is stopped for 3s, the total time is 15 to 20min, the frequency of ultrasonic wave used is 20kHz, and the power is 200W), isoamylase debranching (the reaction pH is 3.5 to 5.0, the reaction temperature is 37 DEG C, and the reaction time is 12 to 16h), alcohol step precipitation fractionation and drying. The method has the advantages of simple processes, low cost, narrow polymerization degree range of the tubular starch derivative products obtained, good embedment characteristic, stable quality and high solubility.

Description

technical field [0001] The invention discloses a method for preparing tubular starch derivatives, which involves the preparation of tubular starch derivatives with a narrow range of polymerization degree by combining ultrasonic clustering starch, isoamylase debranching and ethanol step-by-step precipitation and classification technology, and belongs to the technical field of modified starch processing. Background technique [0002] Tubular starch derivatives are an important class of starch derivatives obtained in recent years. They are in the shape of spiral tubes and have embedding ability. They can replace cyclodextrin as the embedding host molecule and improve the properties of guest stability and water solubility. At present, it is mainly obtained by catalyzing the debranching of amylopectin by debranching enzyme. However, there are two problems with this method: Amylopectin has a cluster structure, which leads to low efficiency of debranching enzyme debranching starc...

Claims

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Application Information

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IPC IPC(8): C12P19/16C12P19/04
Inventor 金征宇田耀旗胡秀婷魏本喜章宝周星徐学明焦爱权
Owner JIANGNAN UNIV
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