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Process for preparing fucoidan by enzymatic hydrolysis of brown algae

A fucoidan sulfate, brown algae technology, applied in biochemical equipment and methods, enzymes, enzymes and other directions, can solve the problems of acid hydrolysis method, such as large ring damage, serious environmental pollution, poor purpose, etc. High rate and purposeful effect

Inactive Publication Date: 2006-04-26
DALIAN FISHERIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The acid hydrolysis method is highly destructive, the environmental pollution is serious, the purpose is poor, and the extraction rate is low; although the cooking method overcomes the shortcomings of the acid hydrolysis method such as high destructiveness and serious pollution, its extraction rate is lower

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A. get 0.46g cellulase and 0.22g pectinase, mix and prepare 0.68g compound cellulase;

[0015] b. claim 200g dry sea-tangle as raw material, add the water of 18 times (3600g) of raw material weight in dry sea-tangle, and add the compound cellulase (accounting for 0.34% of raw material weight) prepared, enzyme under the condition of 8.0 in pH solution for 50 minutes, then heated in a 94°C water bath for 4 hours, lowered to room temperature, and centrifuged to obtain the supernatant;

[0016] c. Add ethanol to the supernatant to produce a precipitate, and centrifuge to obtain the supernatant;

[0017] d. Add ethanol to the supernatant obtained in step c until a precipitate occurs, and collect the precipitate;

[0018] e. The precipitate was washed with ether, and vacuum freeze-dried to obtain a white powder.

[0019] The extracted white powder is fucoidan sulfate, the molecular weight is 10-25 daltons, and the weight is 1.2% of the raw material weight.

Embodiment 2

[0021] A. get 0.44g cellulase and 0.08g pectinase, mix and prepare 0.52 compound cellulase;

[0022] b. claim 200g dry sea-tangle as raw material, add the water of 13 times (2600g) of raw material weight in dry sea-tangle, and add the compound cellulase (accounting for 0.26% of raw material weight) prepared, enzyme under the condition of 6.0 in pH solution for 80 minutes, then heated in a 100°C water bath for 3.5 hours, cooled to room temperature, and centrifuged to obtain the supernatant;

[0023] c. Add 95% ethanol to the supernatant to produce a precipitate, and centrifuge to obtain the supernatant;

[0024] d. Add 95% ethanol to the supernatant obtained in step c until a precipitate occurs, and collect the precipitate;

[0025] e. The precipitate was washed with ether, and vacuum freeze-dried to obtain a white powder.

[0026] The extracted white powder is fucoidan sulfate, the molecular weight is 10-25 daltons, and the weight is 1.2% of the raw material weight.

Embodiment 3

[0028] A. get 0.48g cellulase and 0.16g pectinase, mix and prepare 0.64g compound cellulase;

[0029] B. claim 200g dry sea-tangle as raw material, add the water of 20 times (4000g) of raw material weight in dry sea-tangle, and add the compound cellulase (accounting for 0.32% of raw material weight) prepared, enzyme under the condition of 7.0 in pH solution for 60 minutes, then heated in a 96°C water bath for 3.5 hours, cooled to room temperature, and centrifuged to obtain the supernatant;

[0030] c. Add 95% ethanol to the supernatant to produce a precipitate, and centrifuge to obtain the supernatant;

[0031] d. Add 95% ethanol to the supernatant obtained in step c until a precipitate occurs, and collect the precipitate;

[0032] e. The precipitate was washed with ether, and vacuum freeze-dried to obtain a white powder.

[0033] The extracted white powder is fucoidan sulfate, the molecular weight is 10-25 daltons, and the weight is 1.3% of the raw material weight.

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Abstract

The enzymically hydrolysis process of preparing fucan sulfate with brown algae includes the following steps: compounding composite cellulase with cellulase and pectase; enzymolyzing brown algae material via adding the composite water and cellulase at pH 6.0-8.0 for 50-80 min; heating at 90-100 deg.c in water bath for 3-4 hr, cooling to room temperature and centrifuging to obtain supernatant; adding alcohol to the supernatant to produce precipitate and centrifuging to obtain the second supernatant; adding alcohol to the second supernatant to produce precipitate and collecting the precipitate; washing the precipitate and vacuum freeze drying to obtain white powder. The said process has high extraction rate, low product cost, and less environmental pollution.

Description

Technical field: [0001] The invention relates to a method for preparing fucoidan sulfate, in particular to a method for preparing fucoidan sulfate by enzymatically hydrolyzing brown algae with high extraction rate. Background technique: [0002] Fucoidan sulfate (fucoidan) is a special sulfated polysaccharide that exists in brown algae. Domestic and foreign studies have shown that it has various biological activities such as anticoagulation, lowering blood lipids, antitumor, antivirus, and enhancing the body's immune function. . Because kelp is widely cultivated in my country and its output is huge, accounting for about 89% of the world's output, kelp has become one of the main raw materials for the production of fucoidan sulfate, and the current production methods include acidolysis and cooking. The acid hydrolysis method is highly destructive, causing serious environmental pollution, poor purpose, and low extraction rate; although the cooking method overcomes the shortcom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00C12N9/42
Inventor 汪秋宽何云海陈勇张泽宇康平谢智芬
Owner DALIAN FISHERIES UNIVERSITY
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