Technology for extracting bran dietary fibers by combining ultrasonic-assisted enzymolysis and microbial fermentation
A technology of microbial fermentation and assisted enzymolysis, applied in the field of food processing, can solve the problems of reducing the water holding capacity and swelling property of fiber products, less development and utilization, poor taste, etc., and achieves low production cost, improved nutritional value and low cost. Effect
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Embodiment 1
[0024] The process for extracting bran dietary fiber by combining ultrasonic-assisted enzymolysis and microbial fermentation is characterized in that it specifically includes the following steps:
[0025] (1) Bran pretreatment: pulverize the bran, pass through a 100-mesh sieve, add distilled water at a mass ratio of material to liquid of 1:8, heat to inactivate the enzyme, and obtain a bran suspension;
[0026] (2) Water washing: adjust the pH of the suspension to 5.0-6.0 with citric acid, stir at 45-60°C for 3 hours, decompose phytic acid and extract water-soluble dietary fiber, filter to obtain supernatant a and bran, and then wash the bran until Neutral, adjust the weight ratio of bran and water to be 1:9;
[0027] (3) Ultrasonic-assisted enzymatic hydrolysis to remove starch: adjust the pH of the bran suspension to 5.5-7.5, add α-amylase at a ratio of 4% of the bran mass, and enzymatically hydrolyze under the action of ultrasonic waves, and the enzymatic hydrolysis tempera...
Embodiment 2
[0037] A process for extracting bran dietary fiber by combining ultrasonic-assisted enzymolysis and microbial fermentation, specifically including the following steps:
[0038] (1) Bran pretreatment: pulverize the bran, pass through a 100-mesh sieve, add distilled water at a material-to-liquid mass ratio of 1:10, heat to inactivate the enzyme, and obtain a bran suspension;
[0039] (2) Water washing: adjust the pH of the suspension to 5.0-6.0 with citric acid, stir at 45-60°C for 2 hours, decompose phytic acid and extract water-soluble dietary fiber, filter to obtain supernatant a and bran, and then wash the bran until Neutral, adjust the weight ratio of bran and water to be 1:10;
[0040] (3) Ultrasonic-assisted enzymatic hydrolysis to remove starch: adjust the pH of the bran suspension to 5.5-7.5, add α-amylase at a ratio of 5% of the bran mass, and enzymatically hydrolyze under the action of ultrasonic waves, and the enzymatic hydrolysis temperature is 70-70 80°C, ultrason...
Embodiment 3
[0050] The process for extracting bran dietary fiber by combining ultrasonic-assisted enzymolysis and microbial fermentation is characterized in that it specifically includes the following steps:
[0051] (1) Bran pretreatment: pulverize the bran, pass through a 100-mesh sieve, add distilled water at a material-to-liquid mass ratio of 1:5, heat to inactivate the enzyme, and obtain a bran suspension;
[0052] (2) Water washing: adjust the pH of the suspension to 5.0-6.0 with citric acid, stir at 45-60°C for 4 hours, decompose phytic acid and extract water-soluble dietary fiber, filter to obtain supernatant a and bran, and then wash the bran until Neutral, adjust the weight ratio of bran and water to be 1:8;
[0053] (3) Ultrasonic-assisted enzymatic hydrolysis to remove starch: adjust the pH of the bran suspension to 5.5-7.5, add α-amylase at a ratio of 3% of the bran mass, and enzymatically hydrolyze under the action of ultrasonic waves, and the enzymatic hydrolysis temperatur...
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