Method for Aspergillus oryzae to degrade straw to produce protein feed

A protein feed, Aspergillus oryzae technology, applied in the field of bioengineering, can solve the problems of unsatisfactory treatment effect, large space occupation, long treatment time, etc.

Active Publication Date: 2013-12-18
沂源翰林农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Steam explosion is to use high-pressure steam to infiltrate the interior of the fiber and release it from the closed space in the form of air flow. The fiber undergoes a certain mechanical fracture, and most of the hemicellulose and a small amount of lignin and cellulose in the raw material are degraded and dissolved. The composition of the method is relatively high, large-scale equipment is required, and lignin needs to be treated; the acid treatment method uses sulfuric acid to hydrolyze glycosidic bonds, ester bonds, etc., so that the network structure of corn stalk fibers is loose, ...

Method used

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  • Method for Aspergillus oryzae to degrade straw to produce protein feed
  • Method for Aspergillus oryzae to degrade straw to produce protein feed
  • Method for Aspergillus oryzae to degrade straw to produce protein feed

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1. Production of bacteria on test tube slant

[0036]Insert a ring of Aspergillus oryzae into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technology Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367), at 26°C, and cultivate for 50 hours ; Store at 4°C for later use.

[0037] 2. Production of cultured strains in liquid shake flasks

[0038] Accurately weigh 5 grams of glucose, 0.1 grams of ammonium tartrate, 0.2 grams of benzyl alcohol, 0.2 grams of magnesium sulfate, 0.5 grams of Tween 80, 2 grams of potassium dihydrogen phosphate, 3 grams of phthalic acid buffer, 1000 mL of water, pH 5 , divided into 250mL triangular flasks, 50 grams per bottle, a total of 20 bottles, sterilized at 120 ° C for 40 minutes; after cooling, each bottle was inserted into a ring of Aspergillus oryzae strains preserved at 4 ° C, at 25 ° C, 150 rpm minutes, cultivated for 72 hours;

[0039] 3. Preparation of first-level liquid strains

...

Embodiment 2

[0046] 1. Production of bacteria on test tube slant

[0047] Insert a ring of Aspergillus oryzae into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technology Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367), at 30°C, and cultivate for 100 hours ; Store at 7°C for later use.

[0048] 2. Production of cultured strains in liquid shake flasks

[0049] Accurately weigh 150 grams of glucose, 250 grams of ammonium tartrate, 15 grams of benzyl alcohol, 0.4 grams of magnesium sulfate, 4 grams of Tween 80, 5 grams of potassium dihydrogen phosphate, 20 grams of phthalic acid buffer, 10 L of water, pH 6, Divide into 250mL triangular flasks, 100 grams per bottle, 100 bottles in total, sterilize at 130°C for 30 minutes; after cooling, put a ring of Aspergillus oryzae strains stored at 7°C in each bottle, at 30°C, 150 rpm , cultivated for 50 hours;

[0050] 3. Preparation of first-level liquid strains

[0051] Accurately weigh 2000 gr...

Embodiment 3

[0057] 1. Production of bacteria on test tube slant

[0058] Insert a ring of Aspergillus oryzae into the newly configured potato sucrose medium ("Industrial Microbiology Experimental Technology Manual" edited by Zhuge Jian and Wang Zhengxiang, 1994, page 367), at 35°C, and cultivate for 144 hours ; Store at 10°C for later use.

[0059] 2. Production of cultured strains in liquid shake flasks

[0060] Accurately weigh 300 grams of glucose, 50 grams of ammonium tartrate, 30 grams of benzyl alcohol, 10 grams of magnesium sulfate, 100 grams of Tween 80, 90 grams of potassium dihydrogen phosphate, 180 grams of phthalic acid buffer, 10 L of water, pH7.5 , divided into 250mL triangular flasks, 120 mL per bottle, 80 bottles in total, sterilized at 140°C for 20 minutes; minutes, cultivated for 72 hours;

[0061] 3. Preparation of first-level liquid strains

[0062] Accurately weigh 1,500 grams of glucose, 250 grams of ammonium tartrate, 150 grams of benzyl alcohol, 50 grams of mag...

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Abstract

Relating to the field of biological engineering, the invention provides a method for Aspergillus oryzae to degrade straw to produce protein feed. The method uses straw as a main raw material to produce protein feed by Aspergillus oryzae fermentation. The production process includes: subjecting the straw to crushing, pre-wetting and other pre-treatment technologies, and subjecting the Aspergillus oryzae to liquid shaking culture, first class seed culture, and mixing with straw to undergo solid state fermentation, thus obtaining fermented straw protein feed. The fermentation process is simple, has no pollution to the environment, and also causes no resource waste. The feed prepared by the method has a protein content of 15-30%, a lignin degradation rate of 34-76%, and a feed yield of 45-60%. Because of the effects of Aspergillus oryzae, the feed can degrade lignin and cellulose, thus improving the utilization rate of straw.

Description

technical field [0001] The invention relates to the field of bioengineering, and in particular refers to a process for producing protein feed by degrading lignin and cellulose in the straw without high temperature and high pressure treatment by Aspergillus oryzae. Background technique [0002] my country is a large agricultural country, with an annual output of more than 600 million tons of crop straw, except for a small amount used in the textile and papermaking industries, but most of it is dumped into the environment in the form of accumulation and incineration, which wastes resources and causes great harm to the environment . [0003] Straw contains more than half of the energy accumulated by plant photosynthesis. It is rich in raw sugar, crude protein and other ingredients that are beneficial to livestock and poultry. As a non-competitive feed resource, as long as it can be processed and prepared reasonably, it can be used to feed livestock and poultry. Poultry, especi...

Claims

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Application Information

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IPC IPC(8): A23K1/14
CPCY02P60/87
Inventor 张志才陈钧任晓锋黄达明
Owner 沂源翰林农业发展有限公司
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